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Cited by 233Bacterial Genetics and BiotechnologyIron Metabolism and DisordersDrug Transport and Resistance Mechanisms

The DOI matches its Crossref record; 62 of the 62 references it cites were found in a registry or an index.

FieF (YiiP) from Escherichia coli mediates decreased cellular accumulation of iron and relieves iron stress

Grass, Gregor, Otto, Markus, Fricke, Beate, Haney, Christopher J., Rensing, Christopher, Nies, Dietrich H., Munkelt, Doreen·2005·Archives of Microbiology
DOI 10.1007/s00203-004-0739-4
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Abstract

The Escherichia coli yiiP gene encodes an iron transporter, ferrous iron efflux (FieF), which belongs to the cation diffusion facilitator family (CDF). Transcription of fieF correlated with iron concentration; however, expression appeared to be independent of the ferrous iron uptake regulator Fur. Absence of FieF led to decreased growth of E. coli cells in complex growth medium but only if fur was additionally deleted. The presence of EDTA was partially able to relieve this growth deficiency. Expression of fieF in trans rendered the double deletion strain more tolerant to iron. Furthermore, E. coli cells exhibited reduced accumulation of (55)Fe when FieF was expressed in trans. FieF catalyzed active efflux of Zn(II) in antiport with protons energized by NADH via the transmembrane pH gradient in everted membrane vesicles. Using the iron-sensitive fluorescent indicator PhenGreen-SK encapsulated in proteoliposomes, transmembrane fluxes of iron cations were measured with purified and reconstituted FieF by fluorescence quenching. This suggests that FieF is an iron and zinc efflux system, which would be the first example of iron detoxification by efflux in any organism.

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Metadata

Authors
Grass, G., Otto, M., Fricke, B., Haney, C. J., Rensing, C., Nies, D. H., & Munkelt, D.
Affiliations
  • Martin Luther University Halle-Wittenberg (DE)
  • University of Arizona (US)
Corresponding author
Grass, Gregor
ORCID
Venue
Archives of Microbiology
Publisher
Springer Science and Business Media LLC
Published
2004-11-11
Volume
183
Issue
1
Pages
9-18
ISSN
  • 0003-9276 (ISSN-L)
  • 0302-8933
  • 1432-072X
DOI
10.1007/s00203-004-0739-4
OpenAlex
W2104808495
Cited by
233
References
62
Citations by year
2026: 5 · 2025: 13 · 2024: 12 · 2023: 9 · 2022: 12 · 2021: 6 · 2020: 14 · 2019: 10 · 2018: 13 · 2017: 9 · 2016: 8 · 2015: 11 · 2014: 17 · 2013: 11 · 2012: 12
FWCI
5.71
Type
journal article
Language
en
Open access
closed
Licence
www.springer.com/tdm (tdm)
Locations
  • Archives of Microbiology (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Deutsche Forschungsgemeinschaft
  • National Institute of Environmental Health Sciences (ES 04940, P42ES004940)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
  • Health Sciences › Medicine › Hematology › Iron Metabolism and Disorders
  • Health Sciences › Medicine › Oncology › Drug Transport and Resistance Mechanisms
Keywords
fiefs, cation diffusion facilitator, Escherichia coli, iron stress, iron accumulation, Fur regulator, zinc efflux, antiport, iron detoxification, transmembrane pH gradient, proteoliposomes
MeSH terms
Bacterial Proteins, Biological Transport, Active, Escherichia coli, Fluorescent Dyes, Genetic Complementation Test, Hydrogen-Ion Concentration, Iron, Organic Chemicals, Repressor Proteins, Zinc, Ion Transport, Gene Deletion, Membrane Transport Proteins, Escherichia coli Proteins

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