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Hydrophilic interaction liquid chromatography (HILIC)—a powerful separation technique

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Buszewski, Bogusław, Noga, Sylwia·2012·Analytical and Bioanalytical Chemistry

Abstract

Hydrophilic interaction liquid chromatography (HILIC) provides an alternative approach to effectively separate small polar compounds on polar stationary phases. The purpose of this work was to review the options for the characterization of HILIC stationary phases and their applications for separations of polar compounds in complex matrices. The characteristics of the hydrophilic stationary phase may affect and in some cases limit the choices of mobile phase composition, ion strength or buffer pH value available, since mechanisms other than hydrophilic partitioning could potentially occur. Enhancing our understanding of retention behavior in HILIC increases the scope of possible applications of liquid chromatography. One interesting option may also be to use HILIC in orthogonal and/or two-dimensional separations. Bioapplications of HILIC systems are also presented.

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Metadata

Authors
Buszewski, B., & Noga, S.
Venue
Analytical and Bioanalytical Chemistry
Published
2011-08-31
Volume
402
Issue
1
Pages
231-247
ISSN
  • 1618-2642 (ISSN-L)
  • 1618-2650
OpenAlex
W2015466408
Cited by
1,235
References
1
FWCI
22.30
Type
journal article
Language
en
Open access
hybrid

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CoChat. (2026). Citation check: Hydrophilic interaction liquid chromatography (HILIC)—a powerful separation technique. https://app.cochat.ai/papers/10.1007/s00216-011-5308-5
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Buszewski, B., & Noga, S. (2012). Hydrophilic interaction liquid chromatography (HILIC)—a powerful separation technique. Analytical and Bioanalytical Chemistry. https://doi.org/10.1007/s00216-011-5308-5

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