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Cited by 216Connexins and lens biologyIon channel regulation and functionNeuroscience and Neuropharmacology Research

Functional Expression of the Murine Connexin 36 Gene Coding for a Neuron-Specific Gap Junctional Protein

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Teubner, B., Degen, J., Söhl, G., Güldenagel, M., Bukauskas, F.F., Trexler, E.B., Verselis, V.K., De Zeeuw, C.I. +5 more·2000·The Journal of Membrane Biology
DOI 10.1007/s00232001094PMC PMC3659790
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Abstract

The mouse connexin 36 (Cx36) gene was mapped on chromosome 2 and an identical transcriptional start site was determined in brain and retina on exon I. Rabbit polyclonal antibodies to the presumptive cytoplasmic loop of the Cx36 protein recognized in immunohistochemical analyses Cx36 expression in the retina, olfactory bulb, hippocampus, inferior olive and cerebellum. In olivary neurons strong punctate labeling at dendritic cell contacts and weaker labeling in the cytoplasm of dendrites were shown by immuno electron microscopy. After expression of mouse Cx36 cDNA in human HeLa cells, neurobiotin transfer was increased 1.8-fold and electrical conductance at least 15-fold compared to untransfected HeLa cells. No Lucifer Yellow transfer was detected in either untransfected or Cx36 transfected HeLa cells. Single Cx36 channels in transfected HeLa cells showed a unitary conductance of 14.3 + or - 0. 8 pS. The sensitivity of Cx36 channels to transjunctional voltage was low in both HeLa-Cx36 cells and Xenopus oocytes expressing mouse Cx36. No increased transfer of neurobiotin was detected in heterotypic gap junctions formed by Cx36 and 9 other connexins expressed in HeLa cells. Our results suggest that Cx36 channels function as electrical synapses for transmission of electrical and metabolic signals between neurons in the central nervous system.

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Authors
Teubner, B., Degen, J., Söhl, G., Güldenagel, M., Bukauskas, F. F., Trexler, E. B., Verselis, V. K., De Zeeuw, C. I., Lee, C. G., Kozak, C. A., Petrasch-Parwez, E., Dermietzel, R., & Willecke, K.
Affiliations
  • University of Bonn (DE)
  • Albert Einstein College of Medicine (US)
  • National Institutes of Health (US)
  • Ruhr University Bochum (DE)
ORCID
Venue
The Journal of Membrane Biology
Publisher
Springer Science and Business Media LLC
Published
2000-08
Volume
176
Issue
3
Pages
249-262
ISSN
0022-2631 (ISSN-L)
DOI
10.1007/s00232001094
PMC
PMC3659790
OpenAlex
W2005655563
Cited by
216
References
0
Citations by year
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FWCI
3.97
Type
journal article
Language
en
Open access
Licence
www.springer.com/tdm (tdm)
Locations
  • The Journal of Membrane Biology (publishedVersion)
  • PubMed (publishedVersion)
  • EUR Research Repository (Erasmus University Rotterdam) (publishedVersion)
  • Europe PMC (PubMed Central) (acceptedVersion)
  • PubMed Central (acceptedVersion)
  • EUR Research Repository (Erasmus University Rotterdam) (submittedVersion)
Funders
  • Deutsche Forschungsgemeinschaft (SFB400)
  • Nederlandse Organisatie voor Wetenschappelijk Onderzoek
  • National Institutes of Health
  • National Institute of Neurological Disorders and Stroke (NS367060, R01NS036706)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Connexins and lens biology
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ion channel regulation and function
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuroscience and Neuropharmacology Research
Keywords
connexin 36, gap junctions, electrical synapses, HeLa cells, Xenopus oocytes, heterotypic gap junctions, inferior olive, retina, olfactory bulb, hippocampus, cerebellum, chromosome 2
MeSH terms
Gap Junction delta-2 Protein, Animals, Antibody Specificity, Base Sequence, Biotin, Brain, Carbon Dioxide, Chromosome Mapping, Crosses, Genetic, Electrophysiology, Eye Proteins, Fluorescent Dyes, Genes, HeLa Cells, Humans, Isoquinolines, Membrane Potentials, Mice, Inbred C57BL, Molecular Sequence Data, Muridae, Nerve Tissue Proteins, Neurons, Oocytes, Rabbits, Recombinant Fusion Proteins, Regulatory Sequences, Nucleic Acid, Retina, Transcription, Genetic, Transfection, Xenopus laevis, Ion Channel Gating, Arachidonic Acid, Gap Junctions, Connexins, Mice

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