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Cited by 192Environmental Toxicology and EcotoxicologyAquatic Ecosystems and Phytoplankton DynamicsHeavy Metal Pollution Remediation

The DOI matches its Crossref record; 53 of the 53 references it cites were found in a registry or an index.

The effect of pH on the uptake and toxicity of copper and zinc in a tropical freshwater alga (Chlorella sp.).

Wilde, Karyn L., Stauber, Jennifer L., Markich, Scott J., Franklin, Natasha M., Brown, Paul L.·2006·Archives of Environmental Contamination and Toxicology
DOI 10.1007/s00244-004-0256-0
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Abstract

Copper and zinc toxicity to the freshwater alga Chlorella sp. was determined at a range of pH values (5.5-8.0) in a synthetic softwater (hardness 40-48 mg CaCO(3)/L). The effects of the metals on algal growth (cell division) rate were determined after 48-h exposure at pH 5.5, 6.0, 6.5, 7.0, 7.5, and 8.0. The toxicity of both metals was pH dependent. As pH decreased from 8.0 to 5.5, the copper concentration required to inhibit the algal growth rate by 50% (IC50) increased from 1.0 to 19 microg/L. For zinc, the IC50 increased from 52 to 2,700 microg/L over the same pH range. Changes in solution speciation alone did not explain the increased toxicity observed as the pH increased. Modelled Cu(2+) and Zn(2+) concentrations decreased with increasing pH, whereas toxicity was observed to increase. Measurements of extracellular (cell-bound) metal concentrations support the biotic ligand model (BLM) theory of competition between protons (H(+)) and metals for binding sites at the algal cell surface. Higher extracellular metal concentrations were observed at high pH, indicating reduced competition. Independent of pH, both extracellular and intracellular copper were directly related to growth inhibition in Chlorella sp., whereas zinc toxicity was related to cell-bound zinc only. These findings suggest that the algal cell surface may be considered as the biotic ligand in further development of a chronic BLM with microalgae. Conditional binding constants (log K) were determined experimentally (using measured intracellular metal concentrations) and theoretically (using concentration-response curves) for copper and zinc for Chlorella sp. at selected pH values. Excellent agreement was found indicating the possibility of using concentration-response data to estimate conditional metal-cell binding constants.

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Crossref · 10.1007/s00244-004-0256-0

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53 of 53 references verified0 citation errors · 0 not checkable

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Citations

Cites

Research works printed but not linked: 3

  1. Brown PL, Haworth A, Sharland SM, Tweed CJ (1991) HARPHRQ: an extended version of the geochemical code PHREEQE. NSS/R188, UK Atomic Energy Authority, Oxford
  2. Markich SJ, Brown PL (1999) Thermochemical data (log K) for environmentally-relevant elements. 1. H, Na, K, Ca, Mg, Fe, Mn, U, Al, Pb, Cu and Cd with model fulvic acid (aspartate, citrate, malonate, salicylate and tricarballyate). ANSTO/E735, Australian Nuclear Science and Technology Organisation, Sydney, Australia
  3. Sprague JB, Fogels A (1977) Watch the Y in bioassay. In: Proceedings 3rd Aquatic Toxicity Workshop, 2–3 November 1976, Halifax, NS, Canada, 107–114

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Metadata

Authors
Wilde, K. L., Stauber, J. L., Markich, S. J., Franklin, N. M., & Brown, P. L.
Affiliations
  • Australian Nuclear Science and Technology Organisation (AU)
  • Commonwealth Scientific and Industrial Research Organisation (AU)
Corresponding author
Wilde, Karyn L.
ORCID
Venue
Archives of Environmental Contamination and Toxicology
Publisher
Springer Science and Business Media LLC
Published
2006-03-30
Volume
51
Issue
2
Pages
174-85
ISSN
  • 0090-4341 (ISSN-L)
  • 1432-0703
DOI
10.1007/s00244-004-0256-0
OpenAlex
W2168778829
Cited by
192
References
50
Citations by year
2026: 5 · 2025: 7 · 2024: 6 · 2023: 8 · 2022: 12 · 2021: 12 · 2020: 12 · 2019: 15 · 2018: 9 · 2017: 14 · 2016: 5 · 2015: 9 · 2014: 14 · 2013: 8 · 2012: 7
FWCI
6.76
Type
journal article
Language
en
Open access
closed
Licence
Locations
  • Archives of Environmental Contamination and Toxicology (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Australian Nuclear Science and Technology Organisation
  • Commonwealth Scientific and Industrial Research Organisation
Topics
  • Physical Sciences › Environmental Science › Health, Toxicology and Mutagenesis › Environmental Toxicology and Ecotoxicology
  • Physical Sciences › Environmental Science › Environmental Chemistry › Aquatic Ecosystems and Phytoplankton Dynamics
  • Physical Sciences › Environmental Science › Water Science and Technology › Heavy Metal Pollution Remediation
Keywords
copper toxicity, zinc toxicity, pH dependence, Chlorella, freshwater microalgae, biotic ligand model, metal uptake, metal speciation, conditional stability constants, algal growth inhibition, cell surface binding sites, tropical freshwater
MeSH terms
Chlorella, Copper, Fresh Water, Hydrogen-Ion Concentration, Water Pollutants, Chemical, Zinc

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