Influence of feeding conditions on clavulanic acid production in fed-batch cultivation with medium containing glycerol
Abstract
First, the effect of different levels of nitrogen source on clavulanic acid (CA) production was evaluated in batch cultivations utilizing complex culture medium containing glycerol and three different levels of soy protein isolate (SPI). Cellular growth, evaluated in terms of the rheological parameter K, was highest with a SPI concentration of 30 g.L(-1) (4.42 g.L(-1) N total). However, the highest production of CA (380 mg.L(-1)) was obtained when an intermediate concentration of 20 g.L(-1) of SPI (2.95 g.L(-1) total N) was used. To address this, the influences of volumetric flow rate (F) and glycerol concentration in the complex feed medium (Cs(F)) in fed-batch cultivations were investigated. The best experimental condition for CA production was F=0.01 L.h(-1) and Cs(F)=120 g.L(-1), and under these conditions maximum CA production was practically twice that obtained in the batch cultivation. A single empirical equation was proposed to relate maximum CA production with F and Cs(F) in fed-batch experiments.
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A pH of 6.8 is favorable for obtaining high titers of CA in submerged cultures.
“Previous studies have shown that CA stability increases at slightly acidic pH values [10,11]; several authors found a pH of 6.8 as favorable for obtaining high titers of CA in submerged cultures [5,7,9,12,13,14,15,16].”
From Degradation Kinetics of Clavulanic Acid in Fermentation Broths at Low Temperatures (2019) · cites this paper as [13]
Teodoro et al. (2006) observed different clavulanic acid concentrations (135-380 mg/L) in media containing varied concentrations of soy protein isolate.
“Similar findings were reported by Teodoro et al. (2006) as different clavulanic acid concentrations were observed (135–380 mg/L) in media containing varied concentrations of soy protein isolate.”
From Fermentation Conditions that Affect Clavulanic Acid Production in Streptomyces clavuligerus: A Systematic Review (2016) · cites this paper as Teodoro et al. (2006)
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Ser, H.-L., Law, J. W.-F., Chaiyakunapruk, N., Jacob, S. A., Palanisamy, U. D., Chan, K.-G., Goh, B.-H., & Lee, L.-H. (2016) · Frontiers in Microbiology · doi:10.3389/fmicb.2016.00522 · Cited by 90 · see citing works
Degradation Kinetics of Clavulanic Acid in Fermentation Broths at Low Temperatures
Gómez-Ríos, D., Ramírez-Malule, H., Neubauer, P., Junne, S., & Ríos-Estepa, R. (2019) · Antibiotics · doi:10.3390/antibiotics8010006 · Cited by 17 · see citing works
- Authors
- Teodoro, J. C., Baptista-Neto, A., Cruz-Hernández, I. L., Hokka, C. O., & Badino, A. C.
- Affiliations
- Universidade Federal de São Carlos (BR)
- Venue
- Applied Microbiology and Biotechnology
- Published
- 2006-01-05
- Volume
- 72
- Issue
- 3
- Pages
- 450-455
- ISSN
- 0175-7598 (ISSN-L)
- 1432-0614
- OpenAlex
- W2047633733
- Cited by
- 42
- References
- 0
- Citations by year
- 2024: 2 · 2022: 1 · 2021: 2 · 2019: 2 · 2018: 2 · 2017: 3 · 2016: 2 · 2015: 1 · 2014: 1 · 2013: 3 · 2012: 2
- FWCI
- 2.28
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Applied Microbiology and Biotechnology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Pharmacology › Microbial Natural Products and Biosynthesis
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Enzyme Production and Characterization
- Keywords
- clavulanic acid production, fed-batch fermentation, glycerol, soy protein isolate, nitrogen source, volumetric flow rate, feed concentration
- MeSH terms
- Culture Media, Fermentation, Glycerol, Industrial Microbiology, Streptomyces, Bioreactors, Clavulanic Acid, Soybean Proteins
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