The DOI matches its Crossref record; 30 of the 30 references it cites were found in a registry or an index.
Magnetic particles for the separation and purification of nucleic acids
Open accessAbstract
Nucleic acid separation is an increasingly important tool for molecular biology. Before modern technologies could be used, nucleic acid separation had been a time- and work-consuming process based on several extraction and centrifugation steps, often limited by small yields and low purities of the separation products, and not suited for automation and up-scaling. During the last few years, specifically functionalised magnetic particles were developed. Together with an appropriate buffer system, they allow for the quick and efficient purification directly after their extraction from crude cell extracts. Centrifugation steps were avoided. In addition, the new approach provided for an easy automation of the entire process and the isolation of nucleic acids from larger sample volumes. This review describes traditional methods and methods based on magnetic particles for nucleic acid purification. The synthesis of a variety of magnetic particles is presented in more detail. Various suppliers of magnetic particles for nucleic acid separation as well as suppliers offering particle-based kits for a variety of different sample materials are listed. Furthermore, commercially available manual magnetic separators and automated systems for magnetic particle handling and liquid handling are mentioned.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
A rapid alkaline extraction procedure for screening recombinant plasmid DNA
Birnboim, H. C., & Doly, J. (1979) · Nucleic Acids Research · doi:10.1093/nar/7.6.1513 · Cited by 14,686
Rapid and simple method for purification of nucleic acids
Boom, R., Sol, C. J., Salimans, M. M., Jansen, C. L., Wertheim-van Dillen, P. M., & van der Noordaa, J. (1990) · Journal of Clinical Microbiology · doi:10.1128/jcm.28.3.495-503.1990 · Cited by 5,266
Selective extraction of polyoma DNA from infected mouse cell cultures
Hirt, B. (1967) · Journal of Molecular Biology · doi:10.1016/0022-2836(67)90307-5 · Cited by 5,146
A rapid boiling method for the preparation of bacterial plasmids
Holmes, D. S., & Quigley, M. (1981) · Analytical Biochemistry · doi:10.1016/0003-2697(81)90473-5 · Cited by 3,265
Preparative and analytical purification of DNA from agarose.
Vogelstein, B., & Gillespie, D. (1979) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.76.2.615 · Cited by 1,574
[17] A rapid alkaline extraction method for the isolation of plasmid DNA
Birnboim, H. C. (1983) · Methods in Enzymology · doi:10.1016/0076-6879(83)00059-2 · Cited by 964
Use of magnetic techniques for the isolation of cells
Šafařı́k, I., & Šafařı́ková, M. (1999) · Journal of Chromatography B: Biomedical Sciences and Applications · doi:10.1016/s0378-4347(98)00338-7 · Cited by 617
Magnetic separation techniques in diagnostic microbiology
Olsvik, O., Popovic, T., Skjerve, E., Cudjoe, K. S., Hornes, E., Ugelstad, J., & Uhlén, M. (1994) · Clinical Microbiology Reviews · doi:10.1128/cmr.7.1.43 · Cited by 504
Preparation and application of new monosized polymer particles
Ugelstad, J., Berge, A., Ellingsen, T., Schmid, R., Nilsen, T.-N., Mørk, P. C., Stenstad, P., Hornes, E., & Olsvik, Ø. (1992) · Progress in Polymer Science · doi:10.1016/0079-6700(92)90017-s · Cited by 459
Bionanotechnology based on silica nanoparticles
Tan, W., Wang, K., He, X., Zhao, X. J., Drake, T., Wang, L., & Bagwe, R. P. (2004) · Medicinal Research Reviews · doi:10.1002/med.20003 · Cited by 457
Cited by
Personalized RNA mutanome vaccines mobilize poly-specific therapeutic immunity against cancer
Sahin, U., Derhovanessian, E., Miller, M., Kloke, B.-P., Simon, P., Löwer, M., Bukur, V., Tadmor, A. D., Luxemburger, U., Schrörs, B., et al. (2017) · Nature · doi:10.1038/nature23003 · Cited by 2,488
BNT162b vaccines protect rhesus macaques from SARS-CoV-2
Vogel, A. B., Kanevsky, I., Che, Y., Swanson, K. A., Muik, A., Vormehr, M., Kranz, L. M., Walzer, K. C., Hein, S., Güler, A., et al. (2021) · Nature · doi:10.1038/s41586-021-03275-y · Cited by 694
Functionalized gold nanoparticles: Synthesis, structure and colloid stability
Zhou, J., Ralston, J., Sedev, R., & Beattie, D. A. (2009) · Journal of Colloid and Interface Science · doi:10.1016/j.jcis.2008.12.002 · Cited by 417
Current Nucleic Acid Extraction Methods and Their Implications to Point-of-Care Diagnostics
Ali, N., Rampazzo, R. D. C. P., Costa, A. D. T., & Krieger, M. A. (2017) · BioMed Research International · doi:10.1155/2017/9306564 · Cited by 359
A materials-science perspective on tackling COVID-19
Tang, Z., Kong, N., Zhang, X., Liu, Y., Hu, P., Mou, S., Liljeström, P., Shi, J., Tan, W., Kim, J. S., et al. (2020) · Nature Reviews Materials · doi:10.1038/s41578-020-00247-y · Cited by 303
Metadata
- Authors
- Berensmeier, S.
- Venue
- Applied Microbiology and Biotechnology
- Published
- 2006-10-25
- Volume
- 73
- Issue
- 3
- Pages
- 495-504
- ISSN
- 0175-7598 (ISSN-L)
- 1432-0614
- OpenAlex
- W2061240424
- Cited by
- 593
- References
- 30
- FWCI
- 2.82
- Type
- journal article
- Language
- en
- Open access
- bronze
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Magnetic particles for the separation and purification of nucleic acids. https://app.cochat.ai/papers/10.1007/s00253-006-0675-0
- The paper
- Berensmeier, S. (2006). Magnetic particles for the separation and purification of nucleic acids. Applied Microbiology and Biotechnology. https://doi.org/10.1007/s00253-006-0675-0
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
