The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
Engineering the isobutanol biosynthetic pathway in Escherichia coli by comparison of three aldehyde reductase/alcohol dehydrogenase genes
Open accessAbstract
Biofuels synthesized from renewable resources are of increasing interest because of global energy and environmental problems. We have previously demonstrated production of higher alcohols from Escherichia coli using a 2-keto acid-based pathway. Here, we have compared the effect of various alcohol dehydrogenases (ADH) for the last step of the isobutanol production. E. coli has the yqhD gene which encodes a broad-range ADH. Isobutanol production significantly decreased with the deletion of yqhD, suggesting that the yqhD gene on the genome contributed to isobutanol production. The adh genes of two bacteria and one yeast were also compared in E. coli harboring the isobutanol synthesis pathway. Overexpression of yqhD or adhA in E. coli showed better production than ADH2, a result confirmed by activity measurements with isobutyraldehyde.
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Cites
Construction of Escherichia coli K‐12 in‐frame, single‐gene knockout mutants: the Keio collection
Baba, T., Ara, T., Hasegawa, M., Takai, Y., Okumura, Y., Baba, M., Datsenko, K. A., Tomita, M., Wanner, B. L., & Mori, H. (2006) · Molecular Systems Biology · doi:10.1038/msb4100050 · Cited by 7,858
Non-fermentative pathways for synthesis of branched-chain higher alcohols as biofuels
Atsumi, S., Hanai, T., & Liao, J. C. (2008) · Nature · doi:10.1038/nature06450 · Cited by 1,945
Self-citationThe Complete Genome Sequence of the Lactic Acid Bacterium Lactococcus lactis ssp. lactis IL1403
Bolotin, A., Wincker, P., Mauger, S., Jaillon, O., Malarme, K., Weissenbach, J., Ehrlich, S. D., & Sorokin, A. (2001) · Genome Research · doi:10.1101/gr.gr-1697r · Cited by 1,139
Cited by
Direct photosynthetic recycling of carbon dioxide to isobutyraldehyde
Atsumi, S., Higashide, W., & Liao, J. C. (2009) · Nature Biotechnology · doi:10.1038/nbt.1586 · Cited by 870
Self-citationZymomonas mobilis: a novel platform for future biorefineries
He, M., Wu, B., Qin, H., Ruan, Z., Tan, F., Wang, J., Shui, Z., Dai, L., Zhu, Q., Pan, K., et al. (2014) · Biotechnology for Biofuels · doi:10.1186/1754-6834-7-101 · Cited by 203
Metadata
- Authors
- Atsumi, S., Wu, T.-Y., Eckl, E.-M., Hawkins, S. D., Buelter, T., & Liao, J. C.
- Venue
- Applied Microbiology and Biotechnology
- Published
- 2009-07-16
- Volume
- 85
- Issue
- 3
- Pages
- 651-657
- ISSN
- 0175-7598 (ISSN-L)
- 1432-0614
- OpenAlex
- W1990532591
- Cited by
- 308
- References
- 3
- FWCI
- 9.12
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Atsumi, S., Wu, T.-Y., Eckl, E.-M., Hawkins, S. D., Buelter, T., & Liao, J. C. (2010). Engineering the isobutanol biosynthetic pathway in Escherichia coli by comparison of three aldehyde reductase/alcohol dehydrogenase genes. Applied Microbiology and Biotechnology. https://doi.org/10.1007/s00253-009-2085-6
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