Direct photosynthetic recycling of carbon dioxide to isobutyraldehyde
Abstract
Global climate change has stimulated efforts to reduce CO(2) emissions. One approach to addressing this problem is to recycle CO(2) directly into fuels or chemicals using photosynthesis. Here we genetically engineered Synechococcus elongatus PCC7942 to produce isobutyraldehyde and isobutanol directly from CO(2) and increased productivity by overexpression of ribulose 1,5-bisphosphate carboxylase/oxygenase (Rubisco). Isobutyraldehyde is a precursor for the synthesis of other chemicals, and isobutanol can be used as a gasoline substitute. The high vapor pressure of isobutyraldehyde allows in situ product recovery and reduces product toxicity. The engineered strain remained active for 8 d and produced isobutyraldehyde at a higher rate than those reported for ethanol, hydrogen or lipid production by cyanobacteria or algae. These results underscore the promise of direct bioconversion of CO(2) into fuels and chemicals, which bypasses the need for deconstruction of biomass.
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Engineering the isobutanol biosynthetic pathway and overexpressing Rubisco resulted in enhanced production of isobutyraldehyde and isobutanol (6,230 and 3,000 μg L−1 h−1, respectively) in Synechococcus elongatus PCC 7942.
“Engineering isobutanol biosynthetic pathway and overexpressing Rubisco have resulted in an enhanced production of isobutyraldehayde and isobutanol (6,230 and 3,000 μg L−1 h−1, respectively) in Synechococcus elongatus PCC 7942 (Atsumi et al. 2009).”
From Renewable energy from Cyanobacteria: energy production optimization by metabolic pathway engineering (2011) · cites this paper as Atsumi et al. 2009
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- Authors
- Atsumi, S., Higashide, W., & Liao, J. C.
- Affiliations
- University of California, Los Angeles (US)
- Corresponding author
- Liao, James C
- Venue
- Nature Biotechnology
- Published
- 2009-11-15
- Volume
- 27
- Issue
- 12
- Pages
- 1177-1180
- ISSN
- 0733-222X (ISSN-L)
- 1087-0156
- 1546-1696
- OpenAlex
- W2117741404
- Cited by
- 870
- References
- 36
- Citations by year
- 2026: 18 · 2025: 34 · 2024: 29 · 2023: 38 · 2022: 51 · 2021: 61 · 2020: 49 · 2019: 42 · 2018: 46 · 2017: 55 · 2016: 82 · 2015: 74 · 2014: 75 · 2013: 65 · 2012: 72
- FWCI
- 20.11
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Nature Biotechnology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Photosynthetic Processes and Mechanisms
- Keywords
- CO2 recycling, isobutyraldehyde, isobutanol, Anacystis nidulans, cyanobacteria, photosynthetic carbon fixation, in situ product recovery, biofuels, bio-based chemicals
- MeSH terms
- Aldehydes, Carbon Dioxide, Conservation of Natural Resources, Light, Photosynthesis, Synechococcus, Biofuels
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