Biotechnological production of itaconic acid and its biosynthesis in Aspergillus terreus
Open accessAbstract
More than 80,000 tons of itaconic acid (IA) is produced worldwide each year and is sold at a price of around US$ 2/kg. The IA production yield from sugar is higher than 80 g/l. The widespread use of IA in synthetic resins, synthetic fibers, plastics, rubbers, surfactants, and oil additives has resulted in an increased demand for this product. However, at present, the IA production capacity exceeds the demand because this product has a restricted range of applications. Studies have been actively conducted in different biomedical fields--dental, ophthalmic, and drug delivery--to extend the range of applications of IA. Recently, many researchers have attempted to replace the carbon source used for microbial production of IA with cheaper alternative substrates. However, there is still a need for new biotechnology innovations that would help to reduce the production costs, such as innovative process development and strain improvement to allow the use of a low-quality carbon source. In this short review, we discuss the following aspects of IA production: strain improvement, process development, identification of the key enzyme cis-aconitic acid decarboxylase (CAD) in the IA metabolic pathway, metabolic importance of CAD, and new applications of IA.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
DI potently induces Nrf2 and several Nrf2 target genes in control and LPS-activated BMDMs.
“DI was first employed as an experimental tool and was shown to potently induce Nrf2 and several Nrf2 target genes in control and LPS-activated BMDMs59.”
From Coupling Krebs cycle metabolites to signalling in immunity and cancer (2018) · cites this paper as [59]
Itaconate is an important immunomodulatory metabolite that negatively regulates the inflammatory response and cytokine production.
“Since 2016, several key studies have uncovered a role for itaconate as a crucial anti-inflammatory metabolite that negatively regulates the inflammatory response and cytokine production50,59–61, as shown in Figure 3.”
From Coupling Krebs cycle metabolites to signalling in immunity and cancer (2018) · cites this paper as [59]
DI significantly and acutely depletes GSH levels.
“DI was shown to significantly and acutely deplete GSH levels, confirming it was indeed an activated Michael acceptor59.”
From Coupling Krebs cycle metabolites to signalling in immunity and cancer (2018) · cites this paper as [59]
Itaconate and 4-OI have similar thiol reactivity profiles.
“itaconate and 4-OI were shown to have a similar thiol reactivity profile”
From Coupling Krebs cycle metabolites to signalling in immunity and cancer (2018) · cites this paper as [59]
The ability of 4-OI to induce NQO1 activity remains unaffected when pre-incubated with GSH.
“the ability of 4-OI to induce NQO1 activity remained unaffected59.”
From Coupling Krebs cycle metabolites to signalling in immunity and cancer (2018) · cites this paper as [59]
Induction of Irg1 and itaconate is largely dependent on autocrine/paracrine type I interferon signalling.
“the induction of Irg1 and itaconate was largely dependent on autocrine/paracrine type I interferon signalling59.”
From Coupling Krebs cycle metabolites to signalling in immunity and cancer (2018) · cites this paper as [59]
Pre-treatment of control and LPS-activated macrophages with 4-OI significantly increases Nrf2 and Nrf2 target genes, including HMOX1.
“pre-treatment of control and LPS-activated macrophages with 4-OI still resulted in a significant increase in Nrf2 and Nrf2 target genes, namely Heme oxygenase 1 (HMOX1)”
From Coupling Krebs cycle metabolites to signalling in immunity and cancer (2018) · cites this paper as [59]
Levels of 2,3-dicarboxypropyl cysteine (itaconate-cysteine adduct) are significantly increased in LPS-activated BMDMs.
“Further supporting the hypothesis that itaconate was a thiol reactive metabolite, a significant increase in the levels of the metabolite 2,3-dicarboxypropyl cysteine (itaconate-cysteine adduct), akin to (S)-2-succinocysteine (2SC) a breakdown product of fumarate-mediated protein succination, was observed in LPS-activated BMDMs59.”
From Coupling Krebs cycle metabolites to signalling in immunity and cancer (2018) · cites this paper as [59]
Pre-treatment of LPS-activated BMDMs with 4-OI significantly attenuates IL-1β levels.
“Pre-treatment of LPS-activated BMDMs with 4-OI also resulted in a significant attenuation of IL-1β levels”
From Coupling Krebs cycle metabolites to signalling in immunity and cancer (2018) · cites this paper as [59]
4-OI alkylates KEAP1 on several cysteine residues, including C151.
“4-OI was shown to alkylate KEAP1 on several cysteine residues, namely cysteine 151 (C151), a principal redox sensing cysteine important for activation of Nrf259,66.”
From Coupling Krebs cycle metabolites to signalling in immunity and cancer (2018) · cites this paper as [59]
In LPS- and 4-OI-treated macrophages, several metabolic and inflammatory proteins are alkylated by 2,3-dicarboxypropylation.
“Furthermore, using a quantitative unbiased proteomic screen performed in both LPS (which will drive intracellular itaconate accumulation) and 4-OI-treated macrophages, several metabolic and inflammatory proteins were found to be alkylated representing the first demonstration of this novel post-translational modification (PTM), termed 2,3-dicarboxypropylation59.”
From Coupling Krebs cycle metabolites to signalling in immunity and cancer (2018) · cites this paper as [59]
Pre-treatment of LPS-activated macrophages with 4-OI significantly attenuates IFN-β levels and ISGs, including ISG20.
“pre-treatment of LPS-activated macrophages with 4-OI resulted in a significant attenuation of IFN-β levels and interferon-stimulated genes (ISGs), namely ISG20”
From Coupling Krebs cycle metabolites to signalling in immunity and cancer (2018) · cites this paper as [59]
The attenuation of IFN-β and ISGs by 4-OI is at least partially dependent on Nrf2.
“an effect that was at least partially dependent on Nrf259.”
From Coupling Krebs cycle metabolites to signalling in immunity and cancer (2018) · cites this paper as [59]
The ability of DI to induce NQO1 activity is diminished when DI is pre-incubated with GSH.
“the ability of DI to induce NQO1 activity (a prototypical Nrf2 target gene) was diminished when pre-incubated with GSH”
From Coupling Krebs cycle metabolites to signalling in immunity and cancer (2018) · cites this paper as [59]
The attenuation of IL-1β by 4-OI is largely abrogated in Nrf2-deficient macrophages.
“an effect that was largely abrogated in Nrf2-deficent macrophages59.”
From Coupling Krebs cycle metabolites to signalling in immunity and cancer (2018) · cites this paper as [59]
4-OI is protective in an LPS lethality model in vivo, prolonging survival, improving body temperature regulation and decreasing pro-inflammatory cytokine production.
“4-OI was also shown to be protective in an LPS lethality model in vivo, prolonging survival, improving body temperature regulation and decreasing pro-inflammatory cytokine production59.”
From Coupling Krebs cycle metabolites to signalling in immunity and cancer (2018) · cites this paper as [59]
ACLY is phosphorylated downstream of AKT activation following DNA damage and facilitates histone acetylation at DSB sites, enabling BRCA1 recruitment and DNA repair by homologous recombination.
“ACLY is phosphorylated downstream of AKT activation, following DNA damage and this facilitates histone acetylation at double strand break (DSB) sites, enabling breast cancer early onset 1 (BRCA1) recruitment and DNA repair by homologous recombination.”
From Coupling Krebs cycle metabolites to signalling in immunity and cancer (2018) · cites this paper as [59]
ACLY, acetyl-CoA levels and histone acetylation play an important role in the DNA damage response in tumor cells.
“ACLY, acetyl-CoA levels and histone acetylation have also been shown to play an important role in the DNA damage response in tumor cells 59.”
From Coupling Krebs cycle metabolites to signalling in immunity and cancer (2018) · cites this paper as [59]
ACLY deficiency results in impaired BRCA1 recruitment to DSBs and suppression of homologous recombination.
“ACLY deficiency results in impaired BRCA1 recruitment to sites of DSBs and suppression of homologous recombination.”
From Coupling Krebs cycle metabolites to signalling in immunity and cancer (2018) · cites this paper as [59]
Itaconate primarily acts as a protective, anti-inflammatory metabolite.
“The generation of itaconate from the Krebs Cycle intermediate cis-aconitate contrasts with most other metabolite signals emanating from mitochondria, in that it primarily acts as a protective, anti-inflammatory metabolite55,59,60.”
From Coupling Krebs cycle metabolites to signalling in immunity and cancer (2018) · cites this paper as [59]
4-OI is an itaconate mimic that is protective in a mouse model of sepsis, decreasing cytokine production and increasing survival.
“4-OI is an itaconate mimic that was protective in a mouse model of sepsis, decreasing cytokine production and increasing survival59.”
From Coupling Krebs cycle metabolites to signalling in immunity and cancer (2018) · cites this paper as [59]
Cites
KIRK-OTHMER ENCYCLOPEDIA OF CHEMICAL TECHNOLOGY
(1982) · Chemical & Engineering News · doi:10.1021/cen-v060n014.ibc · Cited by 2,410 · see citing works
Biotechnological production of itaconic acid
Willke, T., & Vorlop, K. (2001) · Applied Microbiology and Biotechnology · doi:10.1007/s002530100685 · Cited by 507 · see citing works
A New Polymer Platform for the Future — Sorona® from Corn Derived 1,3-Propanediol
Kurian, J. V. (2005) · Journal of Polymers and the Environment · doi:10.1007/s10924-005-2947-7 · Cited by 279 · see citing works
PROTEIN SUBCELLULAR LOCALIZATION PREDICTION WITH WOLF PSORT
Horton, P., Park, K.-J., Obayashi, T., & Nakai, K. (2005) · Asia-Pacific Bioinformatics Conference · doi:10.1142/9781860947292_0007 · Cited by 255 · see citing works
Industrial aspects of biochemistry
Spencer, B. (1974) · Elsevier eBooks · Cited by 170 · see citing works
Mutations in yeast HAP2/HAP3 define a hybrid CCAAT box binding domain.
Xing, Y., Fikes, J. D., & Guarente, L. (1993) · The EMBO Journal · doi:10.1002/j.1460-2075.1993.tb06153.x · Cited by 163 · see citing works
Siegel, M. H., Merchuk, J. C., & Schügerl, K. (1986) · AIChE Journal · doi:10.1002/aic.690321002 · Cited by 159 · see citing works
Kanamasa, S., Dwiarti, L., Okabe, M., & Park, E. Y. (2008) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-008-1523-1 · Cited by 130 · see citing works
Self-citationItaconate biosynthesis in Aspergillus terreus
Bonnarme, P., Gillet, B., SEPULCHRE, A. M., Role, C., Beloeil, J. ‐., & Ducrocq, C. (1995) · Journal of Bacteriology · doi:10.1128/jb.177.12.3573-3578.1995 · Cited by 128 · see citing works
Production of Multifunctional Organic Acids from Renewable Resources
Tsao, G. T., Cao, N., Du, J., & Gong, C. S. (1999) · Advances in biochemical engineering, biotechnology · doi:10.1007/3-540-49194-5_10 · Cited by 118 · see citing works
Taşdelen, B., Kayaman-Apohan, N., Güven, O., & Baysal, B. M. (2004) · International Journal of Pharmaceutics · doi:10.1016/j.ijpharm.2004.03.017 · Cited by 111 · see citing works
Studies in the biochemistry of micro-organisms
Calam, C. T., Oxford, A. E., & Raistrick, H. (1939) · Biochemical Journal · doi:10.1042/bj0331488 · Cited by 102 · see citing works
Breeding of Aspergillus terreus mutant TN-484 for itaconic acid production with high yield
Yahiro, K., 高浜, 徹., Park, Y. S., & Okabe, M. (1995) · Journal of Fermentation and Bioengineering · doi:10.1016/0922-338x(95)91272-7 · Cited by 102 · see citing works
Self-citationBIOCHEMISTRY OF THE USTILAGINALES: XI. METABOLIC PRODUCTS OF USTILAGO ZEAE IN SUBMERGED CULTURE
Haskins, R. H., Thorn, J. A., & Boothroyd, B. (1955) · Canadian Journal of Microbiology · doi:10.1139/m55-089 · Cited by 99 · see citing works
Strategies for penicillin fermentation in tower–loop reactors
König, B., Schügerl, K., & Seewald, C. (1982) · Biotechnology and Bioengineering · doi:10.1002/bit.260240202 · Cited by 96 · see citing works
Glass Ionomer Cement – The Different Generations
Upadhya, N. P., & Kishore, G. (2005) · Trends in Biomaterials and Artificial Organs · Cited by 84 · see citing works
BIOSYNTHESIS OF ITACONIC ACID IN ASPERGILLUS TERREUS
Bentley, R., & Thiessen, C. P. (1957) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)70850-8 · Cited by 80 · see citing works
BIOSYNTHESIS OF ITACONIC ACID IN ASPERGILLUS TERREUS
Bentley, R., & Thiessen, C. P. (1957) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)70852-1 · Cited by 77 · see citing works
ITACONIC ACID BY FERMENTATION WITH ASPERGILLUS TERREUS
Pfeifer, V. F., Vojnovich, C., & Heger, E. N. (1952) · Industrial & Engineering Chemistry · doi:10.1021/ie50516a055 · Cited by 76 · see citing works
Efficient itaconic acid production from raw corn starch
Yahiro, K., Shibata, S., Jia, S., Park, Y., & Okabe, M. (1997) · Journal of Fermentation and Bioengineering · doi:10.1016/s0922-338x(97)89265-3 · Cited by 76 · see citing works
Self-citationItaconic acid production by immobilizedAspergillus terreus from xylose and glucose
Kautola, H., Vahvaselk�, M., Linko, Y., & Linko, P. (1985) · Biotechnology Letters · doi:10.1007/bf01027812 · Cited by 75 · see citing works
Reddy, C. S. K., & Singh, R. P. (2002) · Bioresource Technology · doi:10.1016/s0960-8524(02)00075-5 · Cited by 75 · see citing works
Itaconic Acid Fermentation by a Yeast Belonging to the GenusCandida
Tabuchi, T., Sugisawa, T., Ishidori, T., Nakahara, T., & Sugiyama, J. (1981) · Agricultural and Biological Chemistry · doi:10.1080/00021369.1981.10864534 · Cited by 72 · see citing works
Dwiarti, L., Yamane, K., Yamatani, H., Kahar, P., & Okabe, M. (2002) · Journal of Bioscience and Bioengineering · doi:10.1016/s1389-1723(02)80112-8 · Cited by 71 · see citing works
Self-citationŞen, M., & Yakar, A. (2001) · International Journal of Pharmaceutics · doi:10.1016/s0378-5173(01)00804-3 · Cited by 71 · see citing works
Itaconic acid production using sago starch hydrolysate by Aspergillus terreus TN484-M1
Dwiarti, L., Otsuka, M., Miura, S., Yaguchi, M., & Okabe, M. (2007) · Bioresource Technology · doi:10.1016/j.biortech.2006.03.016 · Cited by 67 · see citing works
Self-citationItaconate reduces visceral fat by inhibiting fructose 2,6-bisphosphate synthesis in rat liver
Sakai, A., Kusumoto, A., Kiso, Y., & Furuya, E. (2004) · Nutrition · doi:10.1016/j.nut.2004.08.007 · Cited by 66 · see citing works
Moshaverinia, A., Roohpour, N., Darr, J. A., & Rehman, I. U. (2009) · Acta Biomaterialia · doi:10.1016/j.actbio.2009.02.015 · Cited by 59 · see citing works
Comparison of airlift and stirred reactors for fermentation with Aspergillus niger
Träger, M., Qazi, G. N., Onken, U., & Chopra, C. L. (1989) · Journal of Fermentation and Bioengineering · doi:10.1016/0922-338x(89)90058-5 · Cited by 58 · see citing works
Itaconic acid production in an air-lift bioreactor using a modified draft tube
Okabe, M., Ohta, N., & Park, Y. S. (1993) · Journal of Fermentation and Bioengineering · doi:10.1016/0922-338x(93)90067-i · Cited by 55 · see citing works
Self-citationPark, Y. S., Ohta, N., & Okabe, M. (1993) · Biotechnology Letters · doi:10.1007/bf00138544 · Cited by 47 · see citing works
Self-citationNew polymeric materials for use in glass-ionomer cements
Culbertson, B. M. (2006) · Journal of Dentistry · doi:10.1016/j.jdent.2005.08.008 · Cited by 46 · see citing works
A simple laboratory airlift fermentor
Kiese, S., Ebner, H. G., & Onken, U. (1980) · Biotechnology Letters · doi:10.1007/bf00138667 · Cited by 43 · see citing works
Production of itaconic acid by Aspergillus terreus immobilized in polyacrylamide gels
Horitsu, H., Takahashi, Y., Tsuda, J., Kawai, K., & Kawano, Y. (1983) · Applied Microbiology and Biotechnology · doi:10.1007/bf00504745 · Cited by 43 · see citing works
Itaconic acid production by immobilized Aspergillus terreus with varied metal additions
Kautola, H., Rymowicz, W., Linko, Y., & Linko, P. (1991) · Applied Microbiology and Biotechnology · doi:10.1007/bf00184679 · Cited by 43 · see citing works
BIOSYNTHESIS OF ITACONIC ACID IN ASPERGILLUS TERREUS
Bentley, R., & Thiessen, C. P. (1957) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)70851-x · Cited by 42 · see citing works
Stanojević, M., Krušić, M. K., Filipović, J. M., Parojčić, J., & Stupar, M. (2006) · Drug Delivery · doi:10.1080/10717540500313034 · Cited by 42 · see citing works
Kato, M., Aoyama, A., Naruse, F., Tateyama, Y., Hayashi, K., Miyazaki, M., Papagiannopoulos, P., Davis, M. A., Hynes, M. J., Kobayashi, T., et al. (1998) · Molecular and General Genetics MGG · doi:10.1007/s004380050664 · Cited by 40 · see citing works
Comparison of air-lift and stirred tank reactors for itaconic acid production by Aspergillus terreus
Yahiro, K., Takahama, T., Jai, S.-R., Park, Y. S., & Okabe, M. (1997) · Biotechnology Letters · doi:10.1023/a:1018374428391 · Cited by 39 · see citing works
Self-citationGong, B., & Wang, Y. (2002) · Analytical and Bioanalytical Chemistry · doi:10.1007/s00216-001-1207-5 · Cited by 37 · see citing works
De, T., Bergey, E. J., Chung, S. J., Rodman, D. J., Bharali, D. J., & Prasad, P. N. (2004) · Journal of Microencapsulation · doi:10.1080/02652040400008515 · Cited by 37 · see citing works
Continuous itaconic acid production by immobilized biocatalysts
Kautola, H., Vassilev, N. B., & Linko, Y. (1990) · Journal of Biotechnology · doi:10.1016/0168-1656(90)90079-q · Cited by 36 · see citing works
Ju, N.-H., & Wang, S. (1986) · Applied Microbiology and Biotechnology · doi:10.1007/bf00257025 · Cited by 35 · see citing works
Conversion of citric acid to itaconic acid in a novel liquid membrane bioreactor
Bressler, E., & Braun, S. (2000) · Journal of Chemical Technology & Biotechnology · doi:10.1002/(sici)1097-4660(200001)75:1 · Cited by 35 · see citing works
Some factors affecting the production of itaconic acid by Aspergillus terreus in agitated cultures.
Lockwood, L. B., & NELSON, G. E. N. (1946) · PubMed · Cited by 35 · see citing works
Transdermal application of bupivacaine-loaded poly(acrylamide(A)-co-monomethyl itaconate) hydrogels
Blanco, M. D., Bernardo, M. V., Teijón, C., Sastre, R. L., & Teijón, J. M. (2003) · International Journal of Pharmaceutics · doi:10.1016/s0378-5173(03)00036-x · Cited by 34 · see citing works
Eimhjellen, K. E., & Larsen, H. (1955) · Biochemical Journal · doi:10.1042/bj0600139 · Cited by 33 · see citing works
Ferraboschi, P., Casati, S., Grisenti, P., & Santaniello, E. (1994) · Tetrahedron · doi:10.1016/s0040-4020(01)81120-0 · Cited by 33 · see citing works
Continuous microbial leaching of a pyritic concentrate by Leptospirillum-like bacteria
Helle, U., & Onken, U. (1988) · Applied Microbiology and Biotechnology · doi:10.1007/bf00250411 · Cited by 29 · see citing works
Park, Y., Itida, M., Ohta, N., & Okabe, M. (1994) · Journal of Fermentation and Bioengineering · doi:10.1016/0922-338x(94)90245-3 · Cited by 27 · see citing works
Self-citationGoda, H., Nagase, T., Tanoue, S., Sugiyama, J., Steidl, S., Tüncher, A., Kobayashi, T., Tsukagoshi, N., Brakhage, A. A., & Kato, M. (2005) · Archives of Microbiology · doi:10.1007/s00203-005-0014-3 · Cited by 27 · see citing works
Moser, A. (1991) · Biotechnology and Bioengineering · doi:10.1002/bit.260371111 · Cited by 26 · see citing works
Hydrodynamics and mass transfer characteristics of packed bubble columns
Abraham, M., & Sawant, S. B. (1990) · The Chemical Engineering Journal · doi:10.1016/0300-9467(90)80011-z · Cited by 21 · see citing works
Bubble column research in Japan
Yoshida, F. (1988) · Chemical Engineering & Technology · doi:10.1002/ceat.270110128 · Cited by 19 · see citing works
Fed‐Batch Culture of Saccharomyces cerevisiae in an Airlift Reactor with Net Draft Tube
Wu, J., & Wu, W. (1991) · Biotechnology Progress · doi:10.1021/bp00009a005 · Cited by 19 · see citing works
Biosynthesis of itaconic acid by aspergillus terreus
Shimi, I. R., & Dein, M. S. N. E. (1962) · Archives of Microbiology · doi:10.1007/bf00408905 · Cited by 17 · see citing works
Träger, M., Qazi, G. N., Buse, R., & Onken, U. (1992) · Journal of Fermentation and Bioengineering · doi:10.1016/0922-338x(92)90059-4 · Cited by 17 · see citing works
木下, 広. (1937) · Medical Entomology and Zoology · Cited by 11 · see citing works
Influence of pressure on growth of Pseudomonas fluorescens
Onken, U., & Jostmann, T. (1984) · Biotechnology Letters · doi:10.1007/bf00129301 · Cited by 10 · see citing works
Flagged first, then by citations
10 of the 69 references couldn’t be matched to a known paper.
Cited by
Sorted by citations
Conversion of Biomass into Chemicals over Metal Catalysts
Besson, M., Gallezot, P., & Pinel, C. (2014) · Chemical Reviews · doi:10.1021/cr4002269 · Cited by 1,780 · see citing works
Immune-responsive gene 1 protein links metabolism to immunity by catalyzing itaconic acid production
Michelucci, A., Cordes, T., Ghelfi, J., Pailot, A., Reiling, N., Goldmann, O., Binz, T., Wegner, A., Tallam, A., Rausell, A., et al. (2013) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1218599110 · Cited by 1,191 · see citing works
Catalysis as an Enabling Science for Sustainable Polymers
Zhang, X., Fevre, M., Jones, G. O., & Waymouth, R. M. (2018) · Chemical Reviews · doi:10.1021/acs.chemrev.7b00329 · Cited by 936 · see citing works
Alternative Monomers Based on Lignocellulose and Their Use for Polymer Production
Delidovich, I., Hausoul, P. J. C., Deng, L., Pfützenreuter, R., Rose, M., & Palkovits, R. (2016) · Chemical Reviews · doi:10.1021/acs.chemrev.5b00354 · Cited by 773 · see citing works
Koutinas, A., Vlysidis, A. A., Pleißner, D., Kopsahelis, N., García, I. L., Kookos, I. K., Papanikolaou, S., Kwan, T. H., & Lin, C. S. K. (2014) · Chemical Society Reviews · doi:10.1039/c3cs60293a · Cited by 555 · see citing works
Recovery of carboxylic acids produced by fermentation
López-Garzón, C. S., & Straathof, A. J. J. (2014) · Biotechnology Advances · doi:10.1016/j.biotechadv.2014.04.002 · Cited by 542 · see citing works
The quest for sustainable polyesters – insights into the future
Vilela, C., Sousa, A. F., Fonseca, A. C., Serra, A. C., Coelho, J. F. J., Freire, C. S. R., & Silvestre, A. J. D. (2014) · Polym. Chem. · doi:10.1039/c3py01213a · Cited by 527 · see citing works
Synthesis and utilisation of sugar compounds derived from lignocellulosic biomass
Kobayashi, H., & Fukuoka, A. (2013) · Green Chemistry · doi:10.1039/c3gc00060e · Cited by 489 · see citing works
Transformation of Biomass into Commodity Chemicals Using Enzymes or Cells
Straathof, A. J. J. (2013) · Chemical Reviews · doi:10.1021/cr400309c · Cited by 463 · see citing works
Bio-based polyesters: Recent progress and future prospects
Zhang, Q., Song, M., Xu, Y., Wang, W., Wang, Z., & Zhang, L. (2021) · Progress in Polymer Science · doi:10.1016/j.progpolymsci.2021.101430 · Cited by 433 · see citing works
Liu, J., Wang, S., Peng, Y., Zhu, J., Zhao, W., & Liu, X. (2020) · Progress in Polymer Science · doi:10.1016/j.progpolymsci.2020.101353 · Cited by 417 · see citing works
Coupling Krebs cycle metabolites to signalling in immunity and cancer
Ryan, D. G., Murphy, M. P., Frezza, C., Prag, H. A., Chouchani, E. T., O’Neill, L. A., & Mills, E. L. (2018) · Nature Metabolism · doi:10.1038/s42255-018-0014-7 · Cited by 398 · see citing works
Itaconic acid is a mammalian metabolite induced during macrophage activation
Strelko, C. L., Lu, W., Dufort, F. J., Seyfried, T. N., Chiles, T. C., Rabinowitz, J. D., & Roberts, M. F. (2011) · Journal of the American Chemical Society · doi:10.1021/ja2070889 · Cited by 391 · see citing works
Bio‐based production of C2–C6 platform chemicals
Jang, Y., Kim, B., Shin, J. H., Choi, Y. J., Choi, S., Song, C. W., Lee, J., Park, H. G., & Lee, S. Y. (2012) · Biotechnology and Bioengineering · doi:10.1002/bit.24599 · Cited by 387 · see citing works
Itaconic acid – a versatile building block for renewable polyesters with enhanced functionality
Robert, T., & Friebel, S. (2016) · Green Chemistry · doi:10.1039/c6gc00605a · Cited by 336 · see citing works
Carbohydrates as Organic Raw Materials
Lichtenthaler, F. W. (2010) · Ullmann's Encyclopedia of Industrial Chemistry · doi:10.1002/14356007.n05_n07 · Cited by 325 · see citing works
Derivation and synthesis of renewable surfactants
Foley, P. M., Kermanshahi‐pour, A., Beach, E. S., & Zimmerman, J. B. (2011) · Chemical Society Reviews · doi:10.1039/c1cs15217c · Cited by 305 · see citing works
Becker, J., & Wittmann, C. (2015) · Angewandte Chemie International Edition · doi:10.1002/anie.201409033 · Cited by 295 · see citing works
Advanced Biofuels and Beyond: Chemistry Solutions for Propulsion and Production
Leitner, W., Klankermayer, J., Pischinger, S., Pitsch, H. G., & Kohse‐Höinghaus, K. (2017) · Angewandte Chemie International Edition · doi:10.1002/anie.201607257 · Cited by 288 · see citing works
Itaconic acid – A biotechnological process in change
Klement, T., & Büchs, J. (2012) · Bioresource Technology · doi:10.1016/j.biortech.2012.11.141 · Cited by 283 · see citing works
Recent Development of Biobased Epoxy Resins: A Review
Kumar, S., Samal, S. K., Mohanty, S., & NAYAK, S. K. (2016) · Polymer-Plastics Technology and Engineering · doi:10.1080/03602559.2016.1253742 · Cited by 270 · see citing works
Enzymatic Synthesis of Biobased Polyesters and Polyamides
Jiang, Y. F., & Loos, K. (2016) · Polymers · doi:10.3390/polym8070243 · Cited by 260 · see citing works
The use of renewable feedstock in UV-curable materials – A new age for polymers and green chemistry
Fertier, L., Koleilat, H., Stemmelen, M., Giani, O., Joly‐Duhamel, C., Lapinte, V., & Robin, J. (2013) · Progress in Polymer Science · doi:10.1016/j.progpolymsci.2012.12.002 · Cited by 249 · see citing works
Bioconversion of sugarcane crop residue for value added products – An overview
Sindhu, R., Gnansounou, E., Binod, P., & Pandey, A. (2016) · Renewable Energy · doi:10.1016/j.renene.2016.02.057 · Cited by 246 · see citing works
Microbial metabolites in nutrition, healthcare and agriculture
Singh, R. G., Kumar, M., Mittal, A., & Mehta, P. K. (2017) · 3 Biotech · doi:10.1007/s13205-016-0586-4 · Cited by 236 · see citing works
Microbial production of building block chemicals and polymers
Lee, J. W., Kim, H. U., Choi, S., Yi, J., & Lee, S. Y. (2011) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2011.02.011 · Cited by 232 · see citing works
Bacterial itaconate degradation promotes pathogenicity
Sasikaran, J., Ziemski, M., Zadora, P. K., Fleig, A., & Berg, I. A. (2014) · Nature Chemical Biology · doi:10.1038/nchembio.1482 · Cited by 217 · see citing works
Synthesis and characterization of petroleum and biobased epoxy resins: a review
Kumar, S., Krishnan, S., Mohanty, S., & NAYAK, S. K. (2018) · Polymer International · doi:10.1002/pi.5575 · Cited by 203 · see citing works
Waste biorefineries using filamentous ascomycetes fungi: Present status and future prospects
Ferreira, J. A., Mahboubi, A., Lennartsson, P. R., & Taherzadeh, M. J. (2016) · Bioresource Technology · doi:10.1016/j.biortech.2016.03.018 · Cited by 201 · see citing works
Microbial production of itaconic acid: developing a stable platform for high product concentrations
Kuenz, A., Gallenmüller, Y., Willke, T., & Vorlop, K. (2012) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-012-4221-y · Cited by 198 · see citing works
Product developments in the bio‐based chemicals arena
de Jong, E., Higson, A., Walsh, P., & Wellisch, M. (2012) · Biofuels Bioproducts and Biorefining · doi:10.1002/bbb.1360 · Cited by 182 · see citing works
Biochemistry of microbial itaconic acid production
Steiger, M. G., Blumhoff, M. L., Mattanovich, D., & Sauer, M. (2013) · Frontiers in Microbiology · doi:10.3389/fmicb.2013.00023 · Cited by 185 · see citing works
Ellis, G. A., Klein, W. P., Lasarte‐Aragonés, G., Thakur, M., Walper, S. A., & Medintz, I. L. (2019) · ACS Catalysis · doi:10.1021/acscatal.9b02413 · Cited by 185 · see citing works
Itaconic Acid: The Surprising Role of an Industrial Compound as a Mammalian Antimicrobial Metabolite
Cordes, T., Michelucci, A., & Hiller, K. (2015) · Annual Review of Nutrition · doi:10.1146/annurev-nutr-071714-034243 · Cited by 183 · see citing works
Recovery Processes of Organic Acids from Fermentation Broths in the Biomass-Based Industry
Li, Q., Jiang, X., Feng, X., Wang, J., Sun, C., Zhang, H., Xian, M., & Liu, H. (2016) · Journal of Microbiology and Biotechnology · doi:10.4014/jmb.1505.05049 · Cited by 177 · see citing works
Aspergillus as a versatile cell factory for organic acid production
Yang, L., Lübeck, M., & Lübeck, P. S. (2016) · Fungal Biology Reviews · doi:10.1016/j.fbr.2016.11.001 · Cited by 170 · see citing works
Downstream process development in biotechnological itaconic acid manufacturing
Magalhães, A. I., de Carvalho, J. C., Medina, J. D. C., & Soccol, C. R. (2016) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-016-7972-z · Cited by 166 · see citing works
Biomass-Derived Production of Itaconic Acid as a Building Block in Specialty Polymers
Teleky, B., & Vodnar, D. C. (2019) · Polymers · doi:10.3390/polym11061035 · Cited by 155 · see citing works
Krebs cycle rewired for macrophage and dendritic cell effector functions
Ryan, D. G., & O'Neill, L. A. J. (2017) · FEBS Letters · doi:10.1002/1873-3468.12744 · Cited by 150 · see citing works
Li, A., van Luijk, N., ter Beek, M., Caspers, M. P. M., Punt, P. J., & WERF, M. J. V. D. (2011) · Fungal Genetics and Biology · doi:10.1016/j.fgb.2011.01.013 · Cited by 146 · see citing works
Biomass pretreatment affects Ustilago maydis in producing itaconic acid
Klement, T., Milker, S., Jäger, G., Grande, P. M., de Marı́a, P. D., & Büchs, J. (2012) · Microbial Cell Factories · doi:10.1186/1475-2859-11-43 · Cited by 144 · see citing works
Krull, S., Hevekerl, A., Kuenz, A., & Prüße, U. (2017) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-017-8192-x · Cited by 142 · see citing works
Metabolic engineering of Yarrowia lipolytica for itaconic acid production
Blazeck, J., Hill, A. D., Jamoussi, M., Pan, A., Miller, J., & Alper, H. S. (2015) · Metabolic Engineering · doi:10.1016/j.ymben.2015.09.005 · Cited by 138 · see citing works
Ustilago maydis produces itaconic acid via the unusual intermediate trans ‐aconitate
Geiser, E., Przybilla, S., Friedrich, A., Buckel, W., Wierckx, N. J. P., Blank, L. M., & Bölker, M. (2015) · Microbial Biotechnology · doi:10.1111/1751-7915.12329 · Cited by 138 · see citing works
Biotechnological production of itaconic acid—things you have to know
Kuenz, A., & Krull, S. (2018) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-018-8895-7 · Cited by 138 · see citing works
Németh, B., Doczi, J., Csete, D., Kacso, G., Ravasz, D., Adams, D., Kiss, G., Nagy, A. M., Horvath, G., Tretter, L., et al. (2016) · The FASEB Journal · doi:10.1096/fj.15-279398 · Cited by 136 · see citing works
Natural bio-based monomers for biomedical applications: a review
Birajdar, M. S., Joo, H., Koh, W., & Park, H. (2021) · Biomaterials Research · doi:10.1186/s40824-021-00208-8 · Cited by 135 · see citing works
Food Fight: Role of Itaconate and Other Metabolites in Antimicrobial Defense
Luan, H. H., & Medzhitov, R. (2016) · Cell Metabolism · doi:10.1016/j.cmet.2016.08.013 · Cited by 130 · see citing works
“Today, global production of itaconate from Aspergillus terreus exceeds 40,000 tons and contributes to the preparation of industrial polymers and bioactive compounds in the agriculture, pharmaceutical, and medicine sectors (Okabe et al., 2009).”
BackgroundBiorenewable chemicals: Feedstocks, technologies and the conflict with food production
Bardhan, S. K., Gupta, S., Gorman, M. E., & Haider, M. A. (2015) · Renewable and Sustainable Energy Reviews · doi:10.1016/j.rser.2015.06.013 · Cited by 129 · see citing works
Expression of the Aspergillus terreus itaconic acid biosynthesis cluster in Aspergillus niger
van der Straat, L., Vernooij, M., Lammers, M., van den Berg, W., Schonewille, T., Cordewener, J. H. G., Meer, I. M. V. D., Koops, A. J., & de Graaff, L. H. (2014) · Microbial Cell Factories · doi:10.1186/1475-2859-13-11 · Cited by 127 · see citing works
Microbial production of specialty organic acids from renewable and waste materials
Alonso, S., Rendueles, M., & Dı́az, M. (2014) · Critical Reviews in Biotechnology · doi:10.3109/07388551.2014.904269 · Cited by 127 · see citing works
Yu, C., Cao, Y., Zou, H., & Xian, M. (2010) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-010-2970-z · Cited by 126 · see citing works
Potential renewable oxygenated biofuels for the aviation and road transport sectors
Jenkins, R. W., Munro, M., Nash, S., & Chuck, C. J. (2012) · Fuel · doi:10.1016/j.fuel.2012.08.019 · Cited by 126 · see citing works
Renewable Polymers from Itaconic Acid by Polycondensation and Ring-Opening-Metathesis Polymerization
Winkler, M., Lacerda, T. M., Mack, F., & Meier, M. A. R. (2015) · Macromolecules · doi:10.1021/acs.macromol.5b00052 · Cited by 121 · see citing works
Status of filamentous fungi in integrated biorefineries
Troiano, D. T., Orsat, V., & Dumont, M. (2019) · Renewable and Sustainable Energy Reviews · doi:10.1016/j.rser.2019.109472 · Cited by 121 · see citing works
Influence of the pH on the itaconic acid production with Aspergillus terreus
Hevekerl, A., Kuenz, A., & Vorlop, K. (2014) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-014-6047-2 · Cited by 120 · see citing works
Kumar, S., Krishnan, S., Samal, S. K., Mohanty, S., & NAYAK, S. K. (2017) · Polymer International · doi:10.1002/pi.5399 · Cited by 120 · see citing works
A Sight to Wheat Bran: High Value-Added Products
Katileviciute, A., Plakys, G., Budrevičiūtė, A., Önder, K., Damiati, S., & Kodzius, R. (2019) · Biomolecules · doi:10.3390/biom9120887 · Cited by 116 · see citing works
Bio-Based and Biodegradable Polymeric Materials for a Circular Economy
Oliver-Cuenca, V., Salaris, V., Muñoz-Gimena, P. F., Agüero, Á., Peltzer, M. A., Alcázar, V., Arrieta, M. P., Sempere‐Torregrosa, J., Pavón, C., Samper, M. D., et al. (2024) · Polymers · doi:10.3390/polym16213015 · Cited by 115 · see citing works
Metabolic engineering of Corynebacterium glutamicum for the production of itaconate
Otten, A. D., Brocker, M., & Bott, M. H. (2015) · Metabolic Engineering · doi:10.1016/j.ymben.2015.06.003 · Cited by 111 · see citing works
Blumhoff, M. L., Steiger, M. G., Mattanovich, D., & Sauer, M. (2013) · Metabolic Engineering · doi:10.1016/j.ymben.2013.05.003 · Cited by 109 · see citing works
Metabolic engineering of Saccharomyces cerevisiae for itaconic acid production
Blazeck, J., Miller, J., Pan, A., Gengler, J., Holden, C., Jamoussi, M., & Alper, H. S. (2014) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-014-5895-0 · Cited by 103 · see citing works
Nieder‐Heitmann, M., Haigh, K. F., & Görgens, J. F. (2019) · Journal of Cleaner Production · doi:10.1016/j.jclepro.2019.118039 · Cited by 102 · see citing works
Geiser, E., Wiebach, V., Wierckx, N. J. P., & Blank, L. M. (2014) · Fungal Biology and Biotechnology · doi:10.1186/s40694-014-0002-y · Cited by 102 · see citing works
Patil, D. M., Phalak, G. A., & Mhaske, S. T. (2016) · Designed Monomers & Polymers · doi:10.1080/15685551.2016.1231045 · Cited by 101 · see citing works
Karimi, S., Soofiani, N. M., Mahboubi, A., & Taherzadeh, M. J. (2018) · Sustainability · doi:10.3390/su10093296 · Cited by 100 · see citing works
Mondala, A. H. (2015) · Journal of Industrial Microbiology & Biotechnology · doi:10.1007/s10295-014-1575-4 · Cited by 98 · see citing works
Production and Polymerization of Biobased Acrylates and Analogs
Fouilloux, H., & Thomas, C. M. (2021) · Macromolecular Rapid Communications · doi:10.1002/marc.202000530 · Cited by 95 · see citing works
Enhancing itaconic acid production by Aspergillus terreus
Tevž, G., Benčina, M., & Legiša, M. (2010) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-010-2642-z · Cited by 94 · see citing works
Chen, F., Lukat, P., Iqbal, A., Saile, K., Kaever, V., van den Heuvel, J., Blankenfeldt, W., Büssow, K., & Peßler, F. (2019) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1908770116 · Cited by 93 · see citing works
Bio-Based Thermosetting Resins for Future Generation: A Review
Bobade, S. K., Paluvai, N. R., Mohanty, S., & NAYAK, S. K. (2016) · Polymer-Plastics Technology and Engineering · doi:10.1080/03602559.2016.1185624 · Cited by 92 · see citing works
Kaaniche, F., Hamed, A., Abdel‐Razek, A. S., Wibberg, D., Abdissa, N., Zendah, I., Allouche, N., Mellouli, L., Shaaban, M., & Sewald, N. (2019) · PLoS ONE · doi:10.1371/journal.pone.0217627 · Cited by 92 · see citing works
Teleky, B., & Vodnar, D. C. (2021) · Polymers · doi:10.3390/polym13203574 · Cited by 92 · see citing works
Biocatalytic conversion of lignocellulose to platform chemicals
Jäger, G., & Büchs, J. (2012) · Biotechnology Journal · doi:10.1002/biot.201200033 · Cited by 92 · see citing works
World market and biotechnological production of itaconic acid
da Cruz, J. C., de Castro, A. M., & Sérvulo, E. F. C. (2018) · 3 Biotech · doi:10.1007/s13205-018-1151-0 · Cited by 91 · see citing works
Sollka, L., & Lienkamp, K. (2020) · Macromolecular Rapid Communications · doi:10.1002/marc.202000546 · Cited by 91 · see citing works
Lv, A., Li, Z., Du, F., & Li, Z. (2014) · Macromolecules · doi:10.1021/ma5020066 · Cited by 88 · see citing works
Metabolic specialization in itaconic acid production: a tale of two fungi
Wierckx, N. J. P., Agrimi, G., Lübeck, P. S., Steiger, M. G., Mira, N. P., & Punt, P. J. (2019) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2019.09.014 · Cited by 88 · see citing works
Huang, X., Lü, X., Li, Y., Li, X., & Li, J.-J. (2014) · Microbial Cell Factories · doi:10.1186/s12934-014-0119-y · Cited by 87 · see citing works
New approaches for itaconic acid production: bottlenecks and possible remedies
Bafana, R., & Pandey, R. A. (2017) · Critical Reviews in Biotechnology · doi:10.1080/07388551.2017.1312268 · Cited by 87 · see citing works
Value added products from fermentation of sugars derived from agro-food residues
Díaz, A. B., Blandino, A., & Caro, I. (2017) · Trends in Food Science & Technology · doi:10.1016/j.tifs.2017.10.016 · Cited by 87 · see citing works
Karaffa, L., Díaz, R., Papp, B., Fekete, E., Sándor, E., & Kubicek, C. P. (2015) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-015-6735-6 · Cited by 86 · see citing works
Maaßen, N., Panakova, M. M., Wierckx, N. J. P., Geiser, E., Zimmermann, M., Bölker, M., Klinner, U., & Blank, L. M. (2013) · Engineering in Life Sciences · doi:10.1002/elsc.201300043 · Cited by 86 · see citing works
Anaerobic Fermentation for Production of Carboxylic Acids as Bulk Chemicals from Renewable Biomass
Wang, J., Lin, M., Xu, M., & Yang, S. (2016) · Advances in biochemical engineering, biotechnology · doi:10.1007/10_2015_5009 · Cited by 86 · see citing works
Tehrani, H. H., Becker, J., Bator, I., Saur, K. M., Meyer, S., Lóia, A. C. R., Blank, L. M., & Wierckx, N. J. P. (2019) · Biotechnology for Biofuels · doi:10.1186/s13068-019-1605-6 · Cited by 84 · see citing works
Weignerová, L., Marhol, P., Šimčíková, D., & Vladimír Křen, A. (2011) · Bioresource Technology · doi:10.1016/j.biortech.2011.08.029 · Cited by 84 · see citing works
Interface tailoring by a versatile functionalization platform for nanostructured wood biocomposites
Montanari, C., Olsén, P., & Berglund, L. A. (2020) · Green Chemistry · doi:10.1039/d0gc02768e · Cited by 83 · see citing works
Metabolic engineering of itaconate production in Escherichia coli
Vuoristo, K. S., Mars, A. E., Sangra, J. V., Springer, J., Eggink, G., Sanders, J. P. M., & Weusthuis, R. A. (2014) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-014-6092-x · Cited by 82 · see citing works
Emerging biotechnologies for production of itaconic acid and its applications as a platform chemical
Badal Chandra, S. A. H. A. (2016) · Journal of Industrial Microbiology & Biotechnology · doi:10.1007/s10295-016-1878-8 · Cited by 81 · see citing works
The Impact of Systems Biology on Bioprocessing
Campbell, K., Xia, J., & Nielsen, J. (2017) · Trends in biotechnology · doi:10.1016/j.tibtech.2017.08.011 · Cited by 80 · see citing works
Ali, M. A., Tateyama, S., Oka, Y., Kaneko, D., Okajima, M. K., & Kaneko, T. (2013) · Macromolecules · doi:10.1021/ma400395b · Cited by 77 · see citing works
Itaconic acid production in microorganisms
Zhao, M., Lu, X., Zong, H., Li, J., & Zhuge, B. (2018) · Biotechnology Letters · doi:10.1007/s10529-017-2500-5 · Cited by 77 · see citing works
Teimoori-Boghsani, Y., Ganjeali, A., Cernava, T., Müller, H., Asili, J., & Berg, G. (2020) · Frontiers in Microbiology · doi:10.3389/fmicb.2019.03013 · Cited by 76 · see citing works
Rewiring a secondary metabolite pathway towards itaconic acid production in Aspergillus niger
Hossain, A. H., Li, A., Brickwedde, A., Wilms, L., Caspers, M. P. M., Overkamp, K. M., & Punt, P. J. (2016) · Microbial Cell Factories · doi:10.1186/s12934-016-0527-2 · Cited by 76 · see citing works
Li, A., Pfelzer, N., Zuijderwijk, R., & Punt, P. J. (2012) · BMC Biotechnology · doi:10.1186/1472-6750-12-57 · Cited by 74 · see citing works
Engineering of primary carbon metabolism in filamentous fungi
Chroumpi, T., Mäkelä, M., & de Vries, R. P. (2020) · Biotechnology Advances · doi:10.1016/j.biotechadv.2020.107551 · Cited by 73 · see citing works
Zhang, X., Schwarze, M., Schomäcker, R., van de Krol, R., & Abdi, F. F. (2023) · Nature Communications · doi:10.1038/s41467-023-36574-1 · Cited by 73 · see citing works
Hevekerl, A., Kuenz, A., & Vorlop, K. (2014) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-014-5743-2 · Cited by 72 · see citing works
A Transcription Factor-Based Biosensor for Detection of Itaconic Acid
Hanko, E. K. R., Minton, N. P., & Malys, N. (2018) · ACS Synthetic Biology · doi:10.1021/acssynbio.8b00057 · Cited by 72 · see citing works
Nieder‐Heitmann, M., Haigh, K. F., & Görgens, J. F. (2018) · Bioresource Technology · doi:10.1016/j.biortech.2018.04.075 · Cited by 72 · see citing works
- Authors
- Okabe, M., Lies, D., Kanamasa, S., & Park, E. Y.
- Affiliations
- Musashino Chemical Laboratory, Ltd. (Japan) (JP)
- Chubu University (JP)
- Shizuoka University (JP)
- Corresponding author
- Park, Enoch Y.
- Venue
- Applied Microbiology and Biotechnology
- Published
- 2009-07-21
- Volume
- 84
- Issue
- 4
- Pages
- 597-606
- ISSN
- 0175-7598 (ISSN-L)
- 1432-0614
- OpenAlex
- W2169769082
- Cited by
- 488
- References
- 69
- Citations by year
- 2026: 9 · 2025: 28 · 2024: 35 · 2023: 38 · 2022: 30 · 2021: 35 · 2020: 40 · 2019: 46 · 2018: 28 · 2017: 39 · 2016: 41 · 2015: 30 · 2014: 30 · 2013: 26 · 2012: 15
- FWCI
- 0.81
- Type
- journal article
- Language
- en
- Open access
- green
- hdl.handle.net
- Licence
- cc-by-nc-nd
- www.springer.com/tdm (tdm)
- Locations
- Applied Microbiology and Biotechnology (publishedVersion)
- PubMed (publishedVersion)
- Shizuoka University Repository (Shizuoka University) (submittedVersion, cc-by-nc-nd)
- Institutional Repositories DataBase (IRDB) (submittedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Biochemical and biochemical processes
- Health Sciences › Medicine › Pharmacology › Microbial Natural Products and Biosynthesis
- Keywords
- itaconic acid, Aspergillus terreus, strain improvement, fermentation process development, metabolic pathways, biopolymer applications, dental applications, drug delivery
- MeSH terms
- Aspergillus, Genetic Engineering, Succinates, Biosynthetic Pathways
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
