An exopolysaccharide produced by the novel halophilic bacterium Halomonas stenophila strain B100 selectively induces apoptosis in human T leukaemia cells
Abstract
Microbial exopolysaccharides (EPSs) are highly heterogeneous polymers produced by fungi and bacteria and have recently been attracting considerable attention from biotechnologists because of their potential applications in many fields, including biomedicine. We have screened the antitumoural activity of a panel of sulphated EPSs produced by a newly discovered species of halophilic bacteria. We found that the novel halophilic bacterium Halomonas stenophila strain B100 produced a heteropolysaccharide that, when oversulphated, exerted antitumoural activity on T cell lines deriving from acute lymphoblastic leukaemia (ALL). Only tumour cells were susceptible to apoptosis induced by the sulphated EPS (B100S), whilst primary T cells were resistant. Moreover, freshly isolated primary cells from the blood of patients with ALL were also susceptible to B100S-induced apoptosis. The newly discovered B100S is therefore the first bacterial EPS that has been demonstrated to exert a potent and selective pro-apoptotic effect on T leukaemia cells, and thus, we propose that the search for new antineoplastic drugs should include the screening of other bacterial EPSs, particularly those isolated from halophiles.
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“Only tumor cells were found susceptible to apoptosis induced by the sulphated EPS (B100S), whilst primary T cells were resistant [116].”
BackgroundInduction of apoptosis in cancer cell lines by the Red Sea brine pool bacterial extracts
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- Authors
- Ruiz-Ruiz, C., Srivastava, G. K., Carranza, D., Mata, J. A., Llamas, I., Santamaría, M., Quesada, E., & Molina, I. J.
- Affiliations
- Universidad de Granada (ES)
- Corresponding author
- Molina, Ignacio J.
- Venue
- Applied Microbiology and Biotechnology
- Published
- 2010-10-03
- Volume
- 89
- Issue
- 2
- Pages
- 345-355
- ISSN
- 0175-7598 (ISSN-L)
- 1432-0614
- OpenAlex
- W2051464692
- Cited by
- 98
- References
- 45
- Citations by year
- 2026: 4 · 2025: 8 · 2024: 8 · 2023: 8 · 2022: 11 · 2021: 10 · 2020: 11 · 2019: 8 · 2018: 7 · 2017: 6 · 2016: 2 · 2015: 5 · 2014: 3 · 2013: 2 · 2012: 3
- FWCI
- 3.43
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Applied Microbiology and Biotechnology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Polysaccharides Composition and Applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Cancer Research and Treatments
- Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Seaweed-derived Bioactive Compounds
- Keywords
- exopolysaccharides, halophilic bacteria, sulfated polysaccharides, apoptosis, acute lymphoblastic leukemia, T-cell leukemia, antitumour activity, selective cytotoxicity
- MeSH terms
- Antineoplastic Agents, Cell Cycle, Humans, Molecular Sequence Data, Polysaccharides, Bacterial, Sodium Chloride, Soil Microbiology, Leukemia-Lymphoma, Adult T-Cell, Apoptosis, Halomonas, Cell Line, Tumor
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