Mechanisms of bacillus cereus biofilm formation: An investigation of the physicochemical characteristics of cell surfaces and extracellular proteins
Abstract
Microbial biofilms contribute to biofouling in a wide range of processes from medical implants to processed food. The extracellular polymeric substances (EPS) are implicated in imparting biofilms with structural stability and resistance to cleaning products. Still, very little is known about the structural role of the EPS in Gram-positive systems. Here, we have compared the cell surface and EPS of surface-attached (biofilm) and free-floating (planktonic) cells of Bacillus cereus, an organism routinely isolated from within biofilms on different surfaces. Our results indicate that the surface properties of cells change during biofilm formation and that the EPS proteins function as non-specific adhesions during biofilm formation. The physicochemical traits of the cell surface and the EPS proteins give us an insight into the forces that drive biofilm formation and maintenance in B. cereus.
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The elimination of microbial biofilms is not easy, and its presence displays antimicrobial resistance.
“Therefore, the elimination of microbial biofilms is not easy, and its presence displays antimicrobial resistance [14,15].”
From Antimicrobial and Antibiofilm Activities of Sulfated Polysaccharides from Marine Algae against Dental Plaque Bacteria (2018) · cites this paper as [15]
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References 64 · Cited by 119
- Authors
- Karunakaran, E., & Biggs, C. A.
- Affiliations
- University of Sheffield (GB)
- Corresponding author
- Biggs, Catherine A.
- Venue
- Applied Microbiology and Biotechnology
- Published
- 2010-10-09
- Volume
- 89
- Issue
- 4
- Pages
- 1161-1175
- ISSN
- 0175-7598 (ISSN-L)
- 1432-0614
- OpenAlex
- W2062386436
- Cited by
- 119
- References
- 64
- Citations by year
- 2026: 4 · 2025: 3 · 2024: 5 · 2023: 8 · 2022: 11 · 2021: 13 · 2020: 15 · 2019: 6 · 2018: 7 · 2017: 5 · 2016: 6 · 2015: 12 · 2014: 9 · 2013: 6 · 2012: 6
- FWCI
- 1.95
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Applied Microbiology and Biotechnology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Engineering and Physical Sciences Research Council (EP/E053556/1, EP/E036252/1)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bacterial biofilms and quorum sensing
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bacillus and Francisella bacterial research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Keywords
- Bacillus cereus, biofilm formation, extracellular polymeric substances, cell surface properties, extracellular proteins, planktonic cells, biofouling, Gram-positive bacteria, biofilm stability
- MeSH terms
- Bacillus cereus, Bacterial Adhesion, Bacterial Proteins, Membrane Glycoproteins, United States, Biofilms
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