Antimicrobial and Antibiofilm Activities of Sulfated Polysaccharides from Marine Algae against Dental Plaque Bacteria
Open accessAbstract
Dental plaque biofilms cause various dental diseases; therefore, inhibiting the growths of the dental plaque bacteria which produce biofilms can be a strategy for preventing dental disease. Certain sulfated polysaccharides from marine algae exert antimicrobial activities against human bacterial pathogens in addition to their physiological benefits. On the basis of these observations, the antimicrobial and antibiofilm activities of sulfated polysaccharides from different marine algae were evaluated against dental plaque bacteria. Among the sulfated polysaccharides, a fucoidan from Fucus vesiculosus showed notable antimicrobial activities against the selected dental plaque bacteria, including some foodborne pathogenic bacteria. The minimum inhibitory concentrations were of 125 to 1000 µg mL−1. Regarding the antibiofilm activity, the fucoidan at the concentrations of above 250 µg mL−1 completely suppressed the biofilm formations and planktonic cell growths of Streptococcus mutans and S. sobrinus. However, no eliminative effect on the completed biofilm was observed. The fucoidan consisted of almost fucose base polysaccharide containing approximately 14.0% sulfate content. The average molecular weight of the fucoidan was changed by heat treatment (121 °C for 15 min) and it affected the antimicrobial activity.
AI snapshot
Written from the paper’s full text.
TL;DR
Sulfated polysaccharides from marine algae were screened against dental plaque bacteria, and a crude fucoidan from *Fucus vesiculosus* (F85) showed antimicrobial and antibiofilm activity. F85 inhibited dental plaque bacteria at MICs of 125–1000 µg mL⁻¹ and, above 250 µg mL⁻¹, completely suppressed biofilm formation and planktonic growth of *Streptococcus mutans* and *S. sobrinus*, but did not eliminate completed biofilm.
Key findings
- Among the tested sulfated polysaccharides, only fucoidan F85 from *F. vesiculosus* inhibited all dental plaque bacteria tested, with MICs of 125–1000 µg mL⁻¹; *S. mutans* KCTC 5458 was the most sensitive.
- F85 also inhibited *Listeria monocytogenes* KCTC 13064 (250 µg mL⁻¹), *Staphylococcus aureus* KCTC 3881 (500 µg mL⁻¹), and two lactic acid bacteria (500 µg mL⁻¹ each), but not Gram-negative bacteria; ampicillin MICs for dental plaque bacteria were 0.8–12.5 µg mL⁻¹.
- F85 at ≥250 µg mL⁻¹ completely suppressed biofilm formation and planktonic cell growth of *S. mutans* and *S. sobrinus*; however, no eliminative effect was observed on completed biofilm, and treatment at stage 3 (24 h) had no effect.
- F85 contained approximately 14.0% sulfate and was mostly fucose-based (>87.4% fucose); its weight-average molar mass was ca. 74,214 g mol⁻¹ without heat treatment and decreased to ca. 13,875 g mol⁻¹ after heat treatment (121 °C, 15 min), which affected antimicrobial activity. F95 from the same species showed no antimicrobial activity despite ca. 7.5% sulfate.
Methods
- Eight sulfated polysaccharides were isolated from five brown, two green, and one red edible marine algae, and four commercial fucoidans (F85, F95, M85, U95) plus γ-carrageenan were also tested.
- Antimicrobial activity was screened by broth microdilution MIC against six foodborne pathogenic bacteria, eight dental plaque bacteria, and two lactic acid bacteria; antibiofilm activity was measured by crystal violet staining and planktonic cell counts in 96-well plates.
- Chemical analyses included total sugar, sulfate, pH, composite monosaccharides by HPAEC-PAD, and molecular weight by gel permeation chromatography; statistical comparisons used one-way ANOVA, LSD/Tukey tests, and independent two-sample t-tests (p < 0.05).
Limitations
- Stated: reproducing an artificial dental plaque microbiota in vitro is not easy because many oral bacteria are unculturable, so only a single or small number of combined strains can be tested.
- Stated: F85 did not eliminate completed biofilm, and the time-course data suggested it might not possess a direct killing effect.
- Evident from the text: the study is in vitro only, the antimicrobial mechanism of sulfated polysaccharides was not clearly revealed, and only one screened fucoidan (F85) showed broad activity against the dental plaque bacteria tested.
Model-written summary. Check it against the paper before relying on it.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
0 of 9 cited sources checked · 0 supported · 0 flagged
- Findingown data
The MICs of ampicillin were 0.8 to 12.5 μg mL−1 for the dental plaque bacteria.
“The MICs of ampicillin (as a standard antimicrobial agent) were in the ranges of 0.8 to 12.5 µg mL−1 for the dental plaque bacteria.”
Quote in the paperNot a substantial claim - Findingown data
Fucoidan F85 inhibited the growth of Listeria monocytogenes KCTC 13064 at 250 μg mL−1.
“In addition, the fucoidan F85 inhibited the growths of Listeria monocytogenes KCTC 13,064 (250 µg mL−1), Staphylococcus aureus KCTC 3881 (500 µg mL−1), and the two lactic acid bacteria (500 µg mL−1 in both), but not of the Gram-negative bacteria.”
Quote in the paperNot a substantial claim - Findingown data
Two sulfated polysaccharides from Undaria pinnatifida and Kjellmaniella crassifolia had growth inhibitory effects against Salmonella typhimurium at 1000 μg mL−1.
“Beside the fucoidan F85, the two sulfated polysaccharides from brown algae Undaria pinnatifida and Kjellmaniella crassifolia had growth inhibitory effects against Salmonella typhimurium at a concentration of 1000 µg mL−1 (in both).”
Quote in the paperNot a substantial claim - Findingown data
Fucoidan F85 inhibited the growth of Staphylococcus aureus KCTC 3881 at 500 μg mL−1.
“In addition, the fucoidan F85 inhibited the growths of Listeria monocytogenes KCTC 13,064 (250 µg mL−1), Staphylococcus aureus KCTC 3881 (500 µg mL−1), and the two lactic acid bacteria (500 µg mL−1 in both), but not of the Gram-negative bacteria.”
Quote in the paperNot a substantial claim - Findingown data
Fucoidan F85 did not inhibit the growth of Gram-negative bacteria tested.
“In addition, the fucoidan F85 inhibited the growths of Listeria monocytogenes KCTC 13,064 (250 µg mL−1), Staphylococcus aureus KCTC 3881 (500 µg mL−1), and the two lactic acid bacteria (500 µg mL−1 in both), but not of the Gram-negative bacteria.”
Quote in the paperNot a substantial claim
- Findingown data
S. mutans KCTC 5458 was the most sensitive among the tested bacterial strains.
“S. mutans KCTC 5458 was the most sensitive among the tested bacterial strains.”
Quote in the paperNot a substantial claim - Findingown data
Fucoidan F85 from Fucus vesiculosus showed growth inhibitory effects against all dental plaque bacteria tested, with MICs of 125 to 1000 μg mL−1.
“Among the sulfated polysaccharides, only the fucoidan F85 from Fucus vesiculosus showed growth inhibitory effects against all the dental plaque bacteria tested in this study, with MICs of 125 to 1000 µg mL−1”
Quote in the paperSubstantial claim - Findingown data
Fucoidan F85 inhibited the growth of two lactic acid bacteria at 500 μg mL−1.
“In addition, the fucoidan F85 inhibited the growths of Listeria monocytogenes KCTC 13,064 (250 µg mL−1), Staphylococcus aureus KCTC 3881 (500 µg mL−1), and the two lactic acid bacteria (500 µg mL−1 in both), but not of the Gram-negative bacteria.”
Quote in the paperNot a substantial claim - Findingown data
In PBS, all experimental groups treated with fucoidan F85 and growth control showed similar decrease to ≤2 log CFU mL−1 within 18 h.
“In PBS, all the experimental groups treated with fucoidan F85 and growth control showed a similar tendency; the S. mutans decreased consistently and reached a detection limit of ≤2 log CFU mL−1 within 18 h.”
Quote in the paperSubstantial claim - Findingown data
In BHI broth, S. mutans treated with fucoidan F85 at 250 μg mL−1 decreased to ca. 3.3 log CFU mL−1 over 48 h.
“In contrast, there was no increase in the S. mutans treated with fucoidan at 125 µg mL−1, and the S. mutans treated with fucoidan F85 at 250 µg mL−1 showed a constant decrease to ca 3.3 log CFU mL−1 for 48 h.”
Quote in the paperSubstantial claim - Findingown data
Fucoidan F85 at concentrations above 250 μg mL−1 completely suppressed biofilm formation and planktonic cell growth of S. mutans for 48 h.
“In particular, the biofilm formation and planktonic cells were completely suppressed for 48 h above the concentration of 250 µg mL−1.”
Quote in the paperSubstantial claim - Findingown data
Fucose was dominant (above 87.4%) in the composite monosaccharides of both fucoidans F85 and F95.
“In the composite monosaccharides, fucose was dominant (above 87.4%) in both fucoidans, and galactose, mannose, and xylose were detected in both as well (Table 3).”
Quote in the paperNot a substantial claim - Findingown data
Biofilm formations of E. faecalis KCTC 5289, S. mutans KCTC 5458, and S. sobrinus KCTC 5809 were significantly higher than those of the other strains at both 24 h and 48 h (p < 0.05).
“At both incubation times of 24 h and 48 h, distinct biofilm formations were found in the strains Enterococcus faecalis KCTC 5289, S. mutans KCTC 5458, and S. sobrinus KCTC 5809, and their biofilm formations were significantly higher than those of the other strains (p < 0.05) (Figure 1A).”
Quote in the paperSubstantial claim - Findingown data
The fucoidan F85 consisted of almost fucose base polysaccharide containing approximately 14.0% sulfate content.
“The chemical investigation revealed that the fucoidan F85 consisted of almost fucose base polysaccharide containing approximately 14.0% sulfate content.”
Quote in the paperNot a substantial claim - Findingown data
Fucoidan F85 contained a lower amount of glucose than fucoidan F95.
“Apart from the fact that fucoidan F85 contained a lower amount of glucose, there was not much difference in the contents of the composite monosaccharides between the two compounds.”
Quote in the paperNot a substantial claim - Findingown data
Galactose, mannose, and xylose were detected in both fucoidans F85 and F95.
“In the composite monosaccharides, fucose was dominant (above 87.4%) in both fucoidans, and galactose, mannose, and xylose were detected in both as well (Table 3).”
Quote in the paperNot a substantial claim - Findingown data
Fucoidan F85 at 1000 μg mL−1 suppressed approximately 91.3% of E. faecalis KCTC 5289 biofilm formation for 24 h compared to growth control.
“For E. faecalis KCTC 5289 associated biofilm (Figure 1B), fucoidan F85 at a concentration of 1000 µg mL−1 suppressed approximately 91.3% of biofilm formation for 24 h compared to growth control, and this was accompanied with the growth inhibition of planktonic cells.”
Quote in the paperSubstantial claim - Findingown data
Fucoidan F85 at concentrations above 250 μg mL−1 completely suppressed both biofilm formation and planktonic cell growth of S. mutans and S. sobrinus.
“In summary, the fucoidan F85 at the concentrations of above 250 µg mL−1 completely suppressed both the biofilm formation and planktonic cell growths of S. mutans and S. sobrinus.”
Quote in the paperSubstantial claim - Findingown data
No effect of γ-carrageenan at 5000 μg mL−1 was observed in all tested bacterial strains in this study.
“However, no effect of the γ-carrageenan at even a high-level dose of 5000 µg mL−1 was observed in all the tested bacterial strains in this study (data not shown).”
Quote in the paperNot a substantial claim - Findingown data
The weight-average molar masses (Mws) of fucoidan F85 and F95 without heat treatment were estimated to be ca. 74,214 g mol−1 and 62,080 g mol−1, respectively.
“The weight-average molar masses (Mws) of the fucoidan F85 and F95 without heat treatment were estimated to be ca. 74,214 g mol−1 and 62,080 g mol−1, respectively.”
Quote in the paperSubstantial claim - Findingown data
The suppressive effect of fucoidan F85 on E. faecalis biofilm was lost at 48 h.
“However, the suppressive effect was lost in 48 h.”
Quote in the paperNot a substantial claim - Interpretationreasoning
Inhibiting the initial biofilm formation of dental plaque bacteria can be a strategy for preventing dental caries.
“Inhibiting the initial biofilm formation of dental plaque bacteria can be a strategy for preventing dental caries.”
Quote in the paperSubstantial claim - Interpretationown data
Fucoidan F85 might not possess a direct killing effect on S. mutans.
“As no rapid cell decreases in all the S. mutans treated with fucoidan F85 were observed in BHI broth and the cell decrease of the S. mutans treated with fucoidan F85 was similar with the growth control in PBS; it was suggested that the fucoidan F85 might not possess a direct killing effect”
Quote in the paperSubstantial claim - Interpretationown data
Fucoidan F85 might be a potential in development of antibiofilm agent capable of inhibiting the biofilm formations of S. mutans and S. sobrinus.
“In particular, the fucoidan F85 at the concentrations of above 250 µg mL−1 completely suppressed the biofilm formations and planktonic cell growths of S mutans and S. sobrinus, suggesting that the fucoidan F85 might be a potential in development of antibiofilm agent capable of inhibiting the biofilm formations of the two dental plaque bacteria.”
Quote in the paperSubstantial claim
- May combine two claims. 18
- The quote only partly states this claim. 1
- Classified as probably not a substantial claim. 13
May combine two claims.
Findingown dataFucoidan F95 did not inhibit the growth of any bacterial strains tested, though it is also from F. vesiculosus.
“In contrast, the fucoidan F95 did not inhibit growths of any bacterial strains, though it is also originated from the F. vesiculosus.”
Quote in the paperNot a substantial claimMay combine two claims.
Findingown dataPlanktonic cells of S. mutans proliferated immediately after inoculation, while biofilm formation had a lag phase for 6 h.
“The planktonic cells proliferated after the inoculation immediately, while the biofilm formation seemed to have a lag phase for 6 h.”
Quote in the paperSubstantial claimThe quote only partly states this claim.
Findingown dataIn BHI broth, S. mutans treated with fucoidan F85 at 125 μg mL−1 showed no increase in cell count.
“In contrast, there was no increase in the S. mutans treated with fucoidan at 125 µg mL−1, and the S. mutans treated with fucoidan F85 at 250 µg mL−1 showed a constant decrease to ca 3.3 log CFU mL−1 for 48 h.”
Quote in the paperSubstantial claimMay combine two claims.
Findingown dataThe total sugar contents of the four commercial fucoidans ranged from 83.5 to 90.5%, while those of the sulfated polysaccharides isolated in this study, except for that from C. fragile, ranged from 63.0 to 75.1%.
“The total sugar contents of the four commercial fucoidans ranged from 83.5 to 90.5%, while those of the sulfated polysaccharides isolated in this study, except for that from C. fragile, ranged from 63.0 to 75.1%.”
Quote in the paperNot a substantial claimMay combine two claims.
Findingown dataFor S. mutans KCTC 5458, antibiofilm activity of fucoidan F85 was observed at all tested concentrations (62.5-1000 μg mL−1) and tended to be concentration-dependent.
“For S. mutans KCTC 5458 (Figure 1C), the antibiofilm activity was observed in all the tested concentrations (62.5–1000 µg mL−1), which tended to be concentration-dependent.”
Quote in the paperSubstantial claimProbably not a substantial claim: may not pass S3 (load-bearing). May combine two claims.
Findingown dataAt a concentration of 2.5 mg mL−1, the pH values of fucoidans F85 and M85 were acidic (pH 5.31 and 4.93, respectively), while those of all the isolated sulfated polysaccharides and a commercial fucoidan U95 were in the ranges of 6.05 to 7.81.
“At a concentration of 2.5 mg mL−1, the pH values of fucoidans F85 and M85 were acidic (pH 5.31 and 4.93, respectively), while those of all the isolated sulfated polysaccharides and a commercial fucoidan U95 were in the ranges of 6.05 to 7.81.”
Quote in the paperNot a substantial claimProbably not a substantial claim: may not pass S3 (load-bearing).
FindingcitationThe polysaccharide from Codium fragile prepared with the water extraction method did not contain sulfate.
“However, the polysaccharide from Codium fragile prepared with the water extraction method [22] did not contain sulfate.”
Quote in the paperNot a substantial claim0 of 1 sources checked- cites [22] Cho, 2010, Molecular characterization and biological activities of water soluble sulfated polysaccharides from Enteromorpha prolifera, Food Sci. Biotechnol. source
Not checked in the free run.
May combine two claims.
Findingown dataFor S. sobrinus KCTC 5809, fucoidan F85 had similar activity as for S. mutans in 24 h, but activities at 62.5 and 125 μg mL−1 were lost in 48 h.
“For S. sobrinus KCTC 5809 (Figure 1D), fucoidan F85 had a similar activity as that exhibited for S. mutans in 24 h, whereas the activities of the fucoidan F85 at the concentrations of 62.5 and 125 µg mL−1 were lost in 48 h.”
Quote in the paperSubstantial claimMay combine two claims.
Findingown dataIn a preliminary experiment, the antimicrobial activity of fucoidan F85 decreased without heat treatment (121 °C for 15 min); the MIC for S. mutans was 1000 μg mL−1.
“Furthermore, in a preliminary experiment, the antimicrobial activity of fucoidan F85 decreased without heat treatment (121 °C for 15 min); the MIC for S. mutans was determined to be 1000 µg mL−1 (data not shown).”
Quote in the paperNot a substantial claimProbably not a substantial claim: may not pass S3 (load-bearing).
Findingnone givenThe sulfate content was higher in the brown algae (6.4-14.0%, including commercial fucoidans) than in those from the green and red algae (0-3.0%).
“According to the algal classification based on pigments, the sulfate content was higher in the brown algae (6.4–14.0%, including commercial fucoidans) than in those from the green and red algae (0–3.0%).”
Quote in the paperNot a substantial claimMay combine two claims.
Findingown dataAfter heat treatment (121 °C for 15 min), the Mw of fucoidan F95 was almost unchanged, whereas that of fucoidan F85 notably decreased to ca. 13,875 g mol−1.
“After the heat treatment, the Mw of fucoidan F95 was almost unchanged, whereas that of fucoidan F85 notably decreased to ca. 13,875 g mol−1.”
Quote in the paperSubstantial claimProbably not a substantial claim: may not pass S3 (load-bearing). May combine two claims.
Findingown dataFucoidan F95 exhibited a basic pH (9.46), though it contained 7.5 ± 2.1% sulfate.
“In contrast, fucoidan F95 exhibited a basic pH (9.46), though it contained 7.5 ± 2.1% sulfate.”
Quote in the paperNot a substantial claimMay combine two claims.
Findingown dataBiofilm formation of S. mutans was clearly distinguished from 12 h of incubation and reached nearly a maximal level at 24 h.
“The biofilm formation was clearly distinguished from 12 h of incubation and reached nearly a maximal level at 24 h.”
Quote in the paperSubstantial claimClassified as probably not a substantial claim. May combine two claims.
PremisecitationDental caries is often associated with increases of acidogenic mutans streptococci and lactobacilli, which can metabolize sugars to acid and lead to demineralization of enamel.
“Dental caries is often associated with increases of acidogenic mutans streptococci and lactobacilli, which can metabolize sugars to acid and consequentially lead to the demineralization of enamel [13].”
Quote in the paperSubstantial claim0 of 1 sources checked- cites [13] Marsh, 2006, Dental plaque as a biofilm and a microbial community—Implications for health and disease, BMC Oral Health source
Not checked in the free run.
May combine two claims.
PremisecitationThe elimination of microbial biofilms is not easy, and its presence displays antimicrobial resistance.
“Therefore, the elimination of microbial biofilms is not easy, and its presence displays antimicrobial resistance [14,15].”
Quote in the paperSubstantial claim0 of 2 sources checked- cites [14] Kaplan, 2000, Biofilm formation as microbial development, Annu. Rev. Microbiol. source
Not checked in the free run.
- cites [15] Karunakaran, 2011, Mechanisms of bacillus cereus biofilm formation: An investigation of the physicochemical characteristics of cell surfaces and extracellular proteins, Appl. Microbiol. Biotechnol. source
Not checked in the free run.
May combine two claims.
PremisecitationDental plaque biofilms cause dental caries, periodontitis and gingivitis.
“In dental disease, dental plaque biofilms are known to cause dental caries, periodontitis and gingivitis, though the biofilms are also present on healthy teeth [10].”
Quote in the paperSubstantial claim0 of 1 sources checked- cites [10] Lee, 2013, Synergistic effect of fucoidan with antibiotics against oral pathogenic bacteria, Arch. Oral Biol. source
Not checked in the free run.
Classified as probably not a substantial claim.
Study design and conductcitationThe antibiofilm activities were measured according to the method recommended by O'Toole [30], with slight modifications.
“The biofilm formations of seven dental plaque bacteria (E. faecalis KCTC 5289, S. mutans KCTC 5458, S. mutans KCCM 40105, S. oralis KCCM 41567, S. sanguinis KCTC 5643, S. sobrinus KCTC 5809, and S. sobrinus KCCM 11898) and the antibiofilm activities of fucoidan F85 against selected bacteria (E. faecalis KCTC 5289, S. mutans KCTC 5458, and S. sobrinus KCTC 5809) were measured according to the”
Quote in the paperSubstantial claim0 of 1 sources checked- cites [30] 2011, Microtiter dish biofilm formation assay, J. Vis. Exp.
Not checked in the free run.
Classified as probably not a substantial claim. May combine two claims.
Study design and conductown dataThe values for the biofilm formation of several dental plaque bacteria were statistically assessed with a one-way ANOVA test; a significant difference (p < 0.05) between the means was identified by the least significant difference and Tukey's test using SPSS.
“The values for the biofilm formation of several dental plaque bacteria were statistically assessed with a one-way ANOVA test; a significant difference (p < 0.05) between the means was identified by the least significant difference and Tukey’s test using SPSS (IBM, Armonk, NY, USA).”
Quote in the paperSubstantial claimProbably not a substantial claim: may not pass S3 (load-bearing).
Study design and conductnone givenAll data, except for the pHs, MICs, and Mws of the sulfated polysaccharides, were expressed as the mean ± standard deviation (SD) in triplicate.
“All data, except for the pHs, MICs, and Mws of the sulfated polysaccharides, were expressed as the mean ± standard deviation (SD) in triplicate.”
Quote in the paperNot a substantial claimProbably not a substantial claim: may not pass S3 (load-bearing), S4 (based). May combine two claims.
InterpretationcitationThe electrical properties of the fucoidan could be associated with the sulfate groups and it might affect the antimicrobial activity.
“The electrical properties of the fucoidan could be associated with the sulfate groups and it might affect the antimicrobial activity.”
Quote in the paperNot a substantial claimClassified as probably not a substantial claim. May combine two claims.
Study design and conductown dataThe values for the antibiofilm activities of fucoidan F85 against selected dental plaque bacteria were assessed by independent two-sample t-tests; the significance level between the fucoidan F85 dose-levels and the control was set at p < 0.05.
“The values for the antibiofilm activities of fucoidan F85 against selected dental plaque bacteria were assessed by independent two-sample t-tests; the significance level between the fucoidan F85 dose-levels and the control was set at p < 0.05.”
Quote in the paperSubstantial claimProbably not a substantial claim: may not pass S3 (load-bearing). May combine two claims.
PremisecitationTwo theories explaining antimicrobial action of sulfated polysaccharides (carrageenans) proposed by Yamashita et al. [6]: binding to bacterial surface; trapping nutrients/cationic minerals.
“There are two theories explaining the antimicrobial action of sulfated polysaccharides (carrageenans) proposed by Yamashita et al. [6]: one suggests the binding of the sulfated polysaccharides on the bacterial surface, although in an experiment using 3H-carrageenan, no binding for S. enteritidis was observed; the other suggests the trapping of nutrients or cationic minerals induced by the sulfated”
Quote in the paperNot a substantial claim0 of 1 sources checked- cites [6] Yamashita, 2001, In vitro bacteriostatic effects of dietary polysaccharides, Food Sci. Technol. Res. source
Not checked in the free run.
Probably not a substantial claim: may not pass S3 (load-bearing).
PremisecitationLi et al. [26] described the structure of the fucoidan from F. vesiculosus, which is composed of α-(1,3)-linked fucose with sulfate groups substituted at the C-4 position on some of the fucose residues.
“Li et al. [26] described the structure of the fucoidan from F. vesiculosus, which is composed of α-(1,3)-linked fucose with sulfate groups substituted at the C-4 position on some of the fucose residues.”
Quote in the paperNot a substantial claim0 of 1 sources checked- cites [26] Li, 2008, Fucoidan: Structure and bioactivity, Molecules source
Not checked in the free run.
Classified as probably not a substantial claim.
Study design and conductcitationThe MICs of the sulfated polysaccharides for foodborne pathogenic and dental plaque bacteria were determined according to the broth micro-dilution method guided by CLSI [29], with slight modification.
“The MICs of the sulfated polysaccharides for the foodborne pathogenic and dental plaque bacteria were determined according to the broth micro-dilution method guided by the Clinical and Laboratory Standards Institute (CLSI) [29], with slight modification.”
Quote in the paperSubstantial claim0 of 1 sources checked- cites [29] Clinical and Laboratory Standards Institute (CLSI) (2006). Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria that Grow Aerobically: Approved Standard, CLSI. [7th ed.]. Document M7-A7.
Not checked in the free run.
May combine two claims.
Interpretationown dataNot only sulfate groups, but also the Mws of sulfate polysaccharides, might play crucial roles in their antimicrobial activities.
“As we found, not only sulfate groups, but also the Mws of sulfate polysaccharides, might play crucial roles in their antimicrobial activities.”
Quote in the paperSubstantial claim
The paper relies on this without support.
The antimicrobial action of sulfated polysaccharides has not been clearly revealed.
“In contrast, the antimicrobial action of sulfated polysaccharides has not been clearly revealed.”
The paper relies on this without support.
The industrial utilization of marine algal antimicrobial candidates as antimicrobial agents or food preservatives is not common.
“However, the industrial utilization of these candidates as antimicrobial agents or food preservatives is not common.”
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Sulfated polysaccharides obtained from marine macroalgae have antimicrobial, anti-biofilm, and antifouling properties in dental plaque bacteria models.
“Antimicrobial, anti-biofilm, and antifouling properties of sulfated polysaccharides obtained from marine macroalgae were also studied using dental plaque bacteria as model [39].”
From The Potential of Seaweeds as a Source of Functional Ingredients of Prebiotic and Antioxidant Value (2019) · cites this paper as [39]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
Each contradiction between two passages listed here was confirmed by an independent check.
Plate counts use conflicting denominators
MinorThe time-course method says colony counts were expressed per gram of sample, but the corresponding results report them per milliliter.
“expressed as the logarithmic number of colony forming units per gram sample.”
“the growth control increased from ca. 5.2 log CFU mL−1 (the initial cell count) to ca. 8.6 log CFU mL−1”
Eight dental plaque bacteria claimed, but seven are listed
MinorThe screening results say eight dental plaque bacteria were tested, while the methods identify seven dental plaque bacterial strains.
“six foodborne pathogenic bacteria, eight dental plaque bacteria, and two lactic acid bacteria”
“dental plaque bacteria Enterococcus faecalis KCTC 5289, Streptococcus mutans KCTC 5458, Streptococcus mutans KCCM 40105, Streptococcus oralis KCCM 41567, Streptococcus sobrinus KCTC 5809, Streptococcus sobrinus KCCM 11,898, and Streptococcus sanguinis KCTC 5643”
Eleven algal species claimed, but ten are identified
MinorThe paper identifies eight species used for isolation. The four commercial fucoidans add only two distinct species: two are from Fucus vesiculosus, and one is from Undaria pinnatifida, which is already among the eight. That makes ten distinct species, not eleven.
“The antimicrobial activities of the sulfated polysaccharides from 11 species of marine algae (including four commercial fucoidans) were screened”
“Four commercial fucoidans, crude fucoidan from Fucus vesiculosus (F85, purity: 85%), purified fucoidan from Fucus vesiculosus (F95, purity: 95%), crude fucoidan from Macrocytis pyrifera (M85, purity: 85%) and purified fucoidan from U. pinnatifida (U95, purity: 95%)”
Cites
Biofilm formation as microbial development
O'Toole, G., Kaplan, H. B., & Kolter, R. (2000) · Annual Review of Microbiology · doi:10.1146/annurev.micro.54.1.49 · Cited by 3,571 · see citing works
Microtiter Dish Biofilm Formation Assay
O’Toole, G. A. (2011) · Journal of Visualized Experiments · doi:10.3791/2437 · Cited by 2,184 · see citing works
A note on the determination of the ester sulphate content of sulphated polysaccharides
Dodgson, K. S., & Price, R. (1962) · Biochemical Journal · doi:10.1042/bj0840106 · Cited by 1,645 · see citing works
Fucoidan: Structure and bioactivity
Li, B., Lu, F., Wei, X., & Zhao, R. (2008) · Molecules · doi:10.3390/molecules13081671 · Cited by 1,383 · see citing works
Dental plaque as a biofilm and a microbial community—Implications for health and disease
Marsh, P. D. (2006) · BMC Oral Health · doi:10.1186/1472-6831-6-s1-s14 · Cited by 1,094 · see citing works
Chemical structures and bioactivities of sulfated polysaccharides from marine algae
Jiao, G., Yu, G., Zhang, J., & Ewart, H. S. (2011) · Marine Drugs · doi:10.3390/md9020196 · Cited by 1,054 · see citing works
Ale, M. T., Mikkelsen, J. D., & Meyer, A. S. (2011) · Marine Drugs · doi:10.3390/md9102106 · Cited by 779 · see citing works
Bacterial cell attachment, the beginning of a biofilm
Palmer, J. S., Flint, S., & Brooks, J. D. (2007) · Journal of Industrial Microbiology & Biotechnology · doi:10.1007/s10295-007-0234-4 · Cited by 755 · see citing works
Marine Polysaccharides from Algae with Potential Biomedical Applications
Raposo, M. F. D. J., Morais, A. M. M. B., & Morais, R. M. S. C. (2015) · Marine Drugs · doi:10.3390/md13052967 · Cited by 693 · see citing works
Antimicrobial Action of Compounds from Marine Seaweed
Pérez, M. J., Falqué, E., & Domı́nguez, H. (2016) · Marine Drugs · doi:10.3390/md14030052 · Cited by 663 · see citing works
Bacterial interactions in dental biofilm
Huang, R., Li, M., & Gregory, R. L. (2011) · Virulence · doi:10.4161/viru.2.5.16140 · Cited by 493 · see citing works
Mabeau, S., & Kloareg, B. (1987) · Journal of Experimental Botany · doi:10.1093/jxb/38.9.1573 · Cited by 165 · see citing works
Handbook of Seafood Quality, Safety and Health Applications
(2010) · Wiley eBooks · doi:10.1002/9781444325546 · Cited by 163 · see citing works
Somani, B. L., Khanade, J., & Sinha, R. (1987) · Analytical Biochemistry · doi:10.1016/0003-2697(87)90172-2 · Cited by 120 · see citing works
Karunakaran, E., & Biggs, C. A. (2011) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-010-2919-2 · Cited by 119 · see citing works
Pierre, G., Sopena, V., Juin, C., Mastouri, A., Graber, M., & Maugard, T. (2011) · Biotechnology and Bioprocess Engineering · doi:10.1007/s12257-011-0224-2 · Cited by 101 · see citing works
Cho, M., Yang, C., Kim, S. M., & You, S. (2010) · Food Science and Biotechnology · doi:10.1007/s10068-010-0073-3 · Cited by 90 · see citing works
Antibacterial effect of fucoidan from Sargassum wightii against the chosen human bacterial pathogens
Marudhupandi, T., & Kumar, T. T. A. (2013) · International Current Pharmaceutical Journal · doi:10.3329/icpj.v2i10.16408 · Cited by 87 · see citing works
An examination of the potential of seaweed extracts as functional ingredients in milk
O'Sullivan, A. M. N., O’Callaghan, Y. C., O’Grady, M. N., Waldron, D. S., Smyth, T. J., O’Brien, N. M., & Kerry, J. P. (2014) · International Journal of Dairy Technology · doi:10.1111/1471-0307.12121 · Cited by 86 · see citing works
Synergistic effect of fucoidan with antibiotics against oral pathogenic bacteria
Lee, K.-Y., Jeong, M.-R., Choi, S.-M., Na, S.-S., & Cha, J.-D. (2013) · Archives of Oral Biology · doi:10.1016/j.archoralbio.2012.11.002 · Cited by 65 · see citing works
In vitro Bacteriostatic Effects on Dietary Polysaccharides.
YAMASHITA, S., SUGITA-KONISHI, Y., & SHIMIZU, M. (2001) · Food Science and Technology Research · doi:10.3136/fstr.7.262 · Cited by 59 · see citing works
Rani, V. (2017) · Indian Journal of Pharmaceutical Sciences · doi:10.4172/pharmaceutical-sciences.1000202 · Cited by 43 · see citing works
Li, L., Li, L.-Q., & Guo, C. (2010) · Journal of Medicinal Plants Research · doi:10.5897/jmpr10.518 · Cited by 42 · see citing works
Marine Algal Sources for Treating Bacterial Diseases
swamy, M. (2011) · Advances in food and nutrition research · doi:10.1016/b978-0-12-387669-0.00006-5 · Cited by 28 · see citing works
Naidu, A., & Davidson, P. M. (2000) · CRC Press eBooks · doi:10.1201/9781420039368.ch10 · Cited by 21 · see citing works
Kim, B., Park, J., Kim, D., Kim, Y.-M., Jun, J., Jeong, I., & Chi, Y. (2016) · Journal of Food Science · doi:10.1111/1750-3841.13335 · Cited by 19 · see citing works
Self-citationDietary Fiber Contents of Marine Algae and Extraction Condition of the Fiber
Do, J., Eun-Mi, K., Koo, J.-G., & Jo, K.-S. (1997) · Korean Journal of Fisheries and Aquatic Sciences · Cited by 18 · see citing works
Isolation of antimicrobial agent from the marine algae Cystoseira hakodatensis
Jun, J., Nakajima, S., Yamazaki, K., Kawai, Y., Yasui, H., & Konishi, Y. (2015) · International Journal of Food Science & Technology · doi:10.1111/ijfs.12719 · Cited by 11 · see citing works
Self-citation
Flagged first, then by citations
Research works printed but not linked: 1
- Koo, 1995, Isolation and purification of fucoidans from Laminaria religiose and Undaria pinnatifida in Korea, J. Korean Fish. Soc., 28, 227
Cited by
Sorted by citations
The Potential of Seaweeds as a Source of Functional Ingredients of Prebiotic and Antioxidant Value
Gomez-Zavaglia, A., Prieto Lage, M. A., Jimenez-Lopez, C., Mejuto, J. C., & Simal-Gandara, J. (2019) · Antioxidants · doi:10.3390/antiox8090406 · Cited by 280 · see citing works
Biofilm formation to inhibition: Role of zinc oxide-based nanoparticles
Mahamuni-Badiger, P. P., Patil, P. M., Badiger, M. V., Patel, P. R., Gadgil, B. S. T., Pandit, A. S., & Bohara, R. A. (2019) · Materials Science and Engineering C · doi:10.1016/j.msec.2019.110319 · Cited by 272 · see citing works
A review of the antibacterial activity and mechanisms of plant polysaccharides
Zhou, Y., Chen, X., Chen, T., & Chen, X. (2022) · Trends in Food Science & Technology · doi:10.1016/j.tifs.2022.03.020 · Cited by 229 · see citing works
Therapies from Fucoidan: New Developments
Fitton, J. H., Stringer, D. N., Park, A. Y., & Karpiniec, S. S. (2019) · Marine Drugs · doi:10.3390/md17100571 · Cited by 225 · see citing works
Macroalgae as a Source of Valuable Antimicrobial Compounds: Extraction and Applications
Silva, A., Silva, S. A., Carpena, M., Garcia‐Oliveira, P., Gullón, P., Barroso, M. F., Prieto, M. A., & Simal‐Gándara, J. (2020) · Antibiotics · doi:10.3390/antibiotics9100642 · Cited by 207 · see citing works
Otero, P., Carpena, M., Garcia‐Oliveira, P., Echave, J., Soria-López, A., García-Pérez, P., Fraga‐Corral, M., Cao, H., Nie, S., Xiao, J., et al. (2021) · Critical Reviews in Food Science and Nutrition · doi:10.1080/10408398.2021.1969534 · Cited by 203 · see citing works
Xie, C., Lee, Z. J., Ye, S., Barrow, C. J., Dunshea, F. R., & Suleria, H. A. R. (2023) · Food Reviews International · doi:10.1080/87559129.2023.2212055 · Cited by 140 · see citing works
Chitosan Nanoparticle Encapsulation of Antibacterial Essential Oils
Negi, A., & Kesari, K. K. (2022) · Micromachines · doi:10.3390/mi13081265 · Cited by 124 · see citing works
Antibacterial Properties of Fucoidans from the Brown Algae Fucus vesiculosus L. of the Barents Sea
Ayrapetyan, O. N., Obluchinskaya, E. D., Zhurishkina, E. V., Skorik, Y. А., Лебедев, Д. В., Kulminskaya, A. A., & Lapina, I. M. (2021) · Biology · doi:10.3390/biology10010067 · Cited by 103 · see citing works
Enzyme-assisted extraction of bioactive compounds from brown seaweeds and characterization
Farvin, K. H. S., Alagarsamy, S., Sattari, Z., Al‐Haddad, S., Fakhraldeen, S. A., Al‐Ghunaim, A., & Al‐Yamani, F. (2019) · Journal of Applied Phycology · doi:10.1007/s10811-019-01906-6 · Cited by 93 · see citing works
Zhu, Y., Liu, L., Sun, Z., Ji, Y., Wang, D., Mei, L., Shen, P., Li, Z., Tang, S., Zhang, H., et al. (2021) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2021.03.065 · Cited by 87 · see citing works
Vishwakarma, J., & Vavilala, S. L. (2019) · Journal of Applied Microbiology · doi:10.1111/jam.14364 · Cited by 86 · see citing works
Chaieb, K., Kouidhi, B., Hosawi, S. B. I., Baothman, O. A. S., Zamzami, M. A., & Altayb, H. N. (2022) · Computers in Biology and Medicine · doi:10.1016/j.compbiomed.2022.105517 · Cited by 79 · see citing works
Anti-biofilm activity of marine algae-derived bioactive compounds
Behzadnia, A., Moosavi‐Nasab, M., & Oliyaei, N. (2024) · Frontiers in Microbiology · doi:10.3389/fmicb.2024.1270174 · Cited by 77 · see citing works
Rădulescu, C., Buruleanu, C. L., Nicolescu, C. M., Olteanu, R. L., Bumbac, M., Holban, G. C., & Simal‐Gándara, J. (2020) · Plants · doi:10.3390/plants9111470 · Cited by 77 · see citing works
Negreanu-Pirjol, B.-S., Negreanu–Pîrjol, T., Popoviciu, D. R., Anton, R. E., & Prelipcean, A. (2022) · Pharmaceutics · doi:10.3390/pharmaceutics14091781 · Cited by 75 · see citing works
Cicinskas, E., Begun, M. A., Tiasto, V., Belousov, A. S., Vikhareva, V. V., Mikhailova, V. A., & Kalitnik, A. A. (2019) · Journal of Biomedical Materials Research Part A · doi:10.1002/jbm.a.36812 · Cited by 71 · see citing works
Shanthi, N., Arumugam, P., Murugan, M., Sudhakar, M. P., & Arunkumar, K. (2021) · ACS Omega · doi:10.1021/acsomega.1c03776 · Cited by 69 · see citing works
Wang, L., Jayawardena, T. U., Yang, H.-W., Lee, H.-G., & Jeon, Y. (2020) · Antioxidants · doi:10.3390/antiox9080724 · Cited by 68 · see citing works
Lu, H., Chang, W.-T., Tsai, M., Chen, C.-H., Chen, W., & Mi, F. (2019) · Marine Drugs · doi:10.3390/md17040236 · Cited by 64 · see citing works
Cabral, E. M., Mondala, J. R. M., Oliveira, M., Przyborska, J., Fitzpatrick, S., Rai, D. K., Saravana, P. S., García‐Vaquero, M., O′Shea, D., Devereux, M. M., et al. (2021) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2021.06.182 · Cited by 58 · see citing works
Antimicrobials from Seaweeds for Food Applications
Cabral, E. M., Oliveira, M., Mondala, J. R. M., Curtin, J. F., Tiwari, B. K., & García‐Vaquero, M. (2021) · Marine Drugs · doi:10.3390/md19040211 · Cited by 57 · see citing works
Natural product-based inhibitors of quorum sensing: A novel approach to combat antibiotic resistance
Alum, E. U., Gulumbe, B. H., Izah, S. C., Uti, D. E., Aja, P. M., Igwenyi, I. O., & Offor, C. E. (2025) · Biochemistry and Biophysics Reports · doi:10.1016/j.bbrep.2025.102111 · Cited by 56 · see citing works
A pH-sensitive oxidized-dextran based double drug-loaded hydrogel with high antibacterial properties
Zhang, M., Chen, G., Lei, M., Lei, J., Li, D., & Zheng, H. S. (2021) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2021.03.169 · Cited by 53 · see citing works
Stahl, V., & Vasudevan, K. (2020) · Cureus · doi:10.7759/cureus.6809 · Cited by 53 · see citing works
Quorum Quenching Approaches against Bacterial-Biofilm-Induced Antibiotic Resistance
D’Aquila, P., Rose, E. D., Sena, G., Scorza, A., Cretella, B., Passarino, G., & Bellizzi, D. (2024) · Antibiotics · doi:10.3390/antibiotics13070619 · Cited by 52 · see citing works
Different Antifungal Activity of Anabaena sp., Ecklonia sp., and Jania sp. against Botrytis cinerea
Righini, H., Baraldi, E., Fernández, Y. G., Quintana, A. M., & Roberti, R. (2019) · Marine Drugs · doi:10.3390/md17050299 · Cited by 52 · see citing works
Potential prebiotic and antibacterial activities of fucoidan from Laminaria japonica
Habibi, M. F., Golmakani, M., Eskandari, M. H., & Hosseini, S. M. H. (2024) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2024.131776 · Cited by 50 · see citing works
Raju, P., & Suganthy, N. (2021) · Applied Nanoscience · doi:10.1007/s13204-021-01881-w · Cited by 48 · see citing works
Zhang, T., Wu, S., Ai, C., Wen, C., Liu, Z., Wang, L., Jiang, L., Shen, P., Zhang, G., & Song, S. (2020) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2020.10.219 · Cited by 47 · see citing works
Vishwakarma, J., & Vavilala, S. L. (2020) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-020-10653-5 · Cited by 43 · see citing works
Antimicrobial therapeutics isolated from algal source: retrospect and prospect
Afzal, S., Yadav, A. K., Poonia, A. K., Choure, K., Yadav, A. N., & Pandey, A. (2022) · Biologia · doi:10.1007/s11756-022-01207-3 · Cited by 42 · see citing works
A critical review on pharmacological properties of sulfated polysaccharides from marine macroalgae
Jegadeshwari, B., & Rajaram, R. (2024) · Carbohydrate Polymers · doi:10.1016/j.carbpol.2024.122488 · Cited by 40 · see citing works
Alreshidi, M. M., Badraoui, R., Adnan, M., Patel, M. B., Alotaibi, A., Saeed, M., Ghandourah, M. A., Al-Motair, K. A., Arif, I. A., Albulaihed, Y., et al. (2022) · Algal Research · doi:10.1016/j.algal.2022.102912 · Cited by 37 · see citing works
Recent Advances in Mussel-Inspired Synthetic Polymers as Marine Antifouling Coatings
Manolakis, I., & Azhar, U. (2020) · Coatings · doi:10.3390/coatings10070653 · Cited by 37 · see citing works
Zhan, K., Ji, X., & Luo, L. (2023) · Chemical and Biological Technologies in Agriculture · doi:10.1186/s40538-023-00433-4 · Cited by 36 · see citing works
Xu, D., Xiao, J., Jiang, D., Liu, Y., Gou, Z., Li, J., Shi, M., Wang, X., Guo, Y., Ma, L., et al. (2022) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2022.03.196 · Cited by 34 · see citing works
Ismail, M. M., & Amer, M. S. (2020) · Acta Botanica Brasilica · doi:10.1590/0102-33062020abb0121 · Cited by 36 · see citing works
Bioactive compounds: a goldmine for defining new strategies against pathogenic bacterial biofilms?
Pompilio, A., Scocchi, M., Mangoni, M. L., Shirooie, S., Serio, A., da Costa, Y. F. G., Alves, M. S., Karatoprak, G. Ş., Süntar, I. P., Khan, H. Z., et al. (2022) · Critical Reviews in Microbiology · doi:10.1080/1040841x.2022.2038082 · Cited by 35 · see citing works
Bioactives From Brown Algae: Antioxidant, Anti‐Inflammatory, Anticancer, and Antimicrobial Potential
Fonseca‐Barahona, I., Shahbaz, K., & Baroutian, S. (2025) · ChemBioEng Reviews · doi:10.1002/cben.70007 · Cited by 34 · see citing works
Kumar, A., Buia, M. C., Palumbo, A., Mohany, M., Wadaan, M. A. M., Hozzein, W. N., Beemster, G. T. S., & AbdElgawad, H. (2019) · Environmental Pollution · doi:10.1016/j.envpol.2019.113765 · Cited by 27 · see citing works
Ugya, A. Y., Chen, H., & Wang, Q. (2023) · International Journal of Phytoremediation · doi:10.1080/15226514.2023.2229920 · Cited by 27 · see citing works
Antibiofilm activity of symbiotic Bacillus species associated with marine gastropods
Viju, N., Punitha, S., & Satheesh, S. (2020) · Annals of Microbiology · doi:10.1186/s13213-020-01554-z · Cited by 25 · see citing works
Plant polyphenols, terpenes, and terpenoids in oral health
Arzani, V., Soleimani, M., Fritsch, T., Jacob, U. M., Calabrese, V., & Arzani, A. (2025) · Open Medicine · doi:10.1515/med-2025-1183 · Cited by 24 · see citing works
McGurrin, A., Das, R. S., Soro, A. B., Maguire, J., Flórez-Fernández, N., Domı́nguez, H., Torres, M. D., Tiwari, B. K., & García‐Vaquero, M. (2025) · Marine Drugs · doi:10.3390/md23010046 · Cited by 23 · see citing works
Study of phenolic-polysaccharide interactions in brown seaweed
Lee, Z. J., Xie, C., Ng, K., & Suleria, H. A. R. (2025) · Food Chemistry · doi:10.1016/j.foodchem.2025.143494 · Cited by 23 · see citing works
Vishwakarma, J., Waghela, B., Falcao, B. P., & Vavilala, S. L. (2021) · Applied Biochemistry and Biotechnology · doi:10.1007/s12010-021-03632-7 · Cited by 23 · see citing works
Arumugam, G., Rajaram, R., Khaleelullah, N. S., & Ramanathan, S. (2019) · Biocatalysis and Agricultural Biotechnology · doi:10.1016/j.bcab.2019.101195 · Cited by 24 · see citing works
Abraham, N., Pandey, G., Kolipaka, T., Negi, M., Srinivasarao, D. A., & Srivastava, S. (2025) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2025.141352 · Cited by 22 · see citing works
Naraparaju, S., Kumar, A. G., Parthasarathy, V., & Sankarganesh, P. (2022) · Biomass Conversion and Biorefinery · doi:10.1007/s13399-022-02591-1 · Cited by 22 · see citing works
Bahrami, S., Nateghi, L., Rashidi, L., Nobandegani, B. K., & Ghorbanpour, M. (2025) · Innovative Food Science & Emerging Technologies · doi:10.1016/j.ifset.2025.103975 · Cited by 21 · see citing works
Huang, Y., Jin, Z. C., Wang, Z., Qi, L., Song, S., Zhu, B., & Dong, X. (2021) · Frontiers in Nutrition · doi:10.3389/fnut.2021.686663 · Cited by 21 · see citing works
Antimicrobial Polysaccharides Obtained From Natural Sources
Rocha, G. A., & Ferreira, R. B. R. (2022) · Future Microbiology · doi:10.2217/fmb-2021-0257 · Cited by 21 · see citing works
Yin, A.-Y., Xu, J., Yang, C., & Hsu, S. (2023) · Molecules · doi:10.3390/molecules28052105 · Cited by 21 · see citing works
Nazari, M., Taheri, M., Nouri, F., Bahmanzadeh, M., & Alikhani, M. Y. (2024) · Annals of Clinical Microbiology and Antimicrobials · doi:10.1186/s12941-024-00760-w · Cited by 24 · see citing works
Ghanavati, P. A. Z., Khodadadi, M., & Tadayoni, M. (2022) · Journal of Food Measurement & Characterization · doi:10.1007/s11694-022-01283-8 · Cited by 19 · see citing works
Cao, X., Cheng, X.-W., Liu, Y.-Y., Dai, H., & Gan, R. (2023) · Microbiological Research · doi:10.1016/j.micres.2023.127548 · Cited by 20 · see citing works
Seaweed-derived polysaccharides as antibacterial and antiviral ingredients
Cooney, O. C., Morrin, S. T., Buck, R. H., Owens, R. A., & Hickey, R. M. (2025) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2025.145823 · Cited by 18 · see citing works
Gomathy, V., Venkatesan, M., Narasimman, V., Aavula, T., & Ramachandran, S. (2021) · Journal of Bioactive and Compatible Polymers · doi:10.1177/08839115211014225 · Cited by 17 · see citing works
Graikini, D., Soro, A. B., Saravana, P. S., Tiwari, B. K., & Sánchez, L. (2023) · Marine Drugs · doi:10.3390/md21090478 · Cited by 18 · see citing works
Evaluation of algal active compounds as potent antibiofilm agent
Nag, M., Lahiri, D., Dey, A., Sarkar, T., Joshi, S. J., & Ray, R. R. (2021) · Journal of Basic Microbiology · doi:10.1002/jobm.202100470 · Cited by 18 · see citing works
Smart biomaterials in restorative dentistry: Recent advances and future perspectives
Sun, J., Jiang, J., Huang, Z., Ma, X., Shen, T., Pan, J., & Bi, Z. (2025) · Materials Today Bio · doi:10.1016/j.mtbio.2025.102349 · Cited by 17 · see citing works
Hao, Z., Dai, S., Tan, J., Gao, Y., Sang, Y., & Xue, H. (2023) · Starch - Stärke · doi:10.1002/star.202300116 · Cited by 17 · see citing works
Zhu, Y., Wang, X., Zhao, Y., Wang, J., Ding, L., Wang, Y., & Ren, H. (2023) · ACS ES&T Water · doi:10.1021/acsestwater.2c00582 · Cited by 16 · see citing works
An overview of potential algae-derived bioactive compounds against urease-positive microorganisms
Oliyaei, N., Hashempur, M. H., Zomorodian, K., Tanideh, N., Noori, M., Abbasi, A. A., Mahdavi, M. R., & Iraji, A. (2023) · Algal Research · doi:10.1016/j.algal.2023.103372 · Cited by 16 · see citing works
Hoare, A. H., Tan, S. P., McLoughlin, P., Mulhare, P., & Hughes, H. P. (2019) · Scientific Reports · doi:10.1038/s41598-019-54326-4 · Cited by 15 · see citing works
Natural Products as Antibiofilm Agents
Danquah, C. A., Minkah, P. A. B., Agana, T. A., Moyo, P., Tetteh, M., Duah, I. O., Amankwah, K. B., Somuah, S. O., Ofori, M., & Maharaj, V. J. (2022) · IntechOpen eBooks · doi:10.5772/intechopen.104434 · Cited by 14 · see citing works
Satessa, G. D., Tamez-Hidalgo, P., Kjærulff, S., Vargas‐Bello‐Pérez, E., Dhakal, R., & Nielsen, M. O. (2020) · Animals · doi:10.3390/ani10040559 · Cited by 14 · see citing works
Moreno‐Chamba, B., Salazar‐Bermeo, J., Martínez‐Madrid, M. C., Lizama, V., Martı́n, F., Berná, G., Neacsu, M., Saura, D., Martí, N., & Valero, M. (2021) · LWT · doi:10.1016/j.lwt.2021.113011 · Cited by 13 · see citing works
Deniz, N., Sarıtaş, S., Bechelany, M., & Karav, S. (2025) · International Journal of Molecular Sciences · doi:10.3390/ijms262211082 · Cited by 16 · see citing works
Mougin, J., Pavaux, A., Fanesi, A., Lopez, J., Pruvost, É., Guihéneuf, F., Sciandra, A., Briandet, R., & Lopes, F. (2024) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-023-12960-z · Cited by 12 · see citing works
Antibiofilm, Antifouling, and Anticorrosive Biomaterials and Nanomaterials for Marine Applications
Jayaprakashvel, M., Mnif, S., & Subramani, R. (2020) · Nanotechnology in the life sciences · doi:10.1007/978-3-030-40337-9_10 · Cited by 12 · see citing works
Achmad, H., Huldani, H., & Ramadhany, Y. F. (2020) · Hasanuddin University Repository · Cited by 12 · see citing works
Antibacterial Polysaccharides in Dental Implantology
Hallmann, L., & Gerngroß, M. D. (2025) · Marine Drugs · doi:10.3390/md23080321 · Cited by 11 · see citing works
Surits, V. V., Usoltseva, R. V., Шевченко, Н. М., Thinh, P. D., & Ermakova, S. P. (2020) · Chemistry of Natural Compounds · doi:10.1007/s10600-020-02938-y · Cited by 11 · see citing works
Vinosha, M., Palanisamy, S., Jeneeta, S., Rajasekar, P., Marudhupandi, T., Muthusamy, K., Mohandoss, S., You, S., & Prabhu, N. M. (2024) · Food Bioscience · doi:10.1016/j.fbio.2024.104420 · Cited by 10 · see citing works
Mousaie, M., Khodadadi, M., & Tadayoni, M. (2022) · Journal of Food Processing and Preservation · doi:10.1111/jfpp.17020 · Cited by 10 · see citing works
Sodium cellulose sulfate and its antimicrobial activity
Normakhamatov, N. S., Mischnick, P., Muhitdinov, B. I., Mukhamedov, I., & Turaev, A. S. (2023) · Reactive and Functional Polymers · doi:10.1016/j.reactfunctpolym.2023.105672 · Cited by 10 · see citing works
Blamo, P. A., Pham, H. N. T., & Nguyen, T. H. (2020) · AIMS Agriculture and Food · doi:10.3934/agrfood.2021003 · Cited by 10 · see citing works
Effects of UV/H2O2 Degradation on the Physicochemical and Antibacterial Properties of Fucoidan
He, Z., Zhu, B., Deng, L., & You, L. (2024) · Marine Drugs · doi:10.3390/md22050209 · Cited by 9 · see citing works
Roy, A., Roy, P. K., Cho, S., & Park, S. Y. (2025) · Microorganisms · doi:10.3390/microorganisms13040914 · Cited by 9 · see citing works
Khan, R. T., Bhuiya, S., Anil, A., Logheeswaran, J., Prathiviraj, R., Selvin, J., & Kiran, G. S. (2025) · Food Bioscience · doi:10.1016/j.fbio.2025.107159 · Cited by 9 · see citing works
Advances in fucoidan extraction and structure-bioactivity relationships.
Mensah, E. O., Sommer-Knudsen, J., Thaysen-Andersen, M., & Sunna, A. (2026) · Carbohydrate Polymers · doi:10.1016/j.carbpol.2026.125134 · Cited by 9 · see citing works
Keskinkaya, H. B., Devecı, E., Güneş, E., Okudan, E. Ş., Akköz, C., Gümüş, N. E., & Karakurt, S. (2022) · Commagene Journal of Biology · doi:10.31594/commagene.1084336 · Cited by 9 · see citing works
Anti-Biofilm Extracts and Molecules from the Marine Environment
Caudal, F., Roullier, C., Rodrigues, S., Dufour, A., Artigaud, S., Blay, G. L., Bazire, A., & Petek, S. (2024) · Marine Drugs · doi:10.3390/md22070313 · Cited by 8 · see citing works
Félix, S. P., Larios, G. S., Cabrera, R., García-Galaz, A., Huerta‐Ocampo, J. Á., Guzmán-Partida, A. M., Corral, R. I. A., Sarabia-Sainz, J. A., & Montfort, G. R.-C. (2025) · Polysaccharides · doi:10.3390/polysaccharides6020024 · Cited by 7 · see citing works
Antimicrobial potential of biopolymers against foodborne pathogens: An updated review
Parvez, M. A. K., Jubyda, F. T., Karmakar, J., Jahan, A., Nayeem-E-Akter, Ayaz, M., Kabir, T., Akter, S., & Huq, M. A. (2025) · Microbial Pathogenesis · doi:10.1016/j.micpath.2025.107583 · Cited by 7 · see citing works
Fucoidan-based pharmaceutical formulations for the delivery of phytochemicals: A review
Bora, S., Medicherla, K., Kulhari, H., & Pooja, D. (2025) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2025.149279 · Cited by 8 · see citing works
Natural Anti-Biofilm Agents: A Comprehensive Review and Future Perspectives
Jeyachandran, S., & Sekar, S. (2025) · Current Microbiology · doi:10.1007/s00284-025-04684-2 · Cited by 7 · see citing works
jasem, M., Merai, A. A., & Nizam, A. A. (2023) · Access Microbiology · doi:10.1099/acmi.0.000537.v5 · Cited by 7 · see citing works
Yu, J., Ding, B., Li, R., Chen, X., Yin, D., Song, M., & Ye, X. (2023) · The Science of The Total Environment · doi:10.1016/j.scitotenv.2023.168963 · Cited by 7 · see citing works
Palpperumal, S., Sankaralingam, S., Balachandran, C., Mahendran, S., Venkatesh, S., Alharbi, N. S. H. A., Thiruvengadam, M., Duraipandiyan, V., & Baskar, K. (2024) · Indian Journal of Microbiology · doi:10.1007/s12088-024-01237-2 · Cited by 7 · see citing works
Fucoidans from Laminaria hyperborea demonstrate bactericidal activity against diverse bacteria
Beagan, M. L. C., Bang, L. L., Pettersen, J. S., Grønnemose, R. B., Foertsch, S., Andersen, T. E., & Ding, M. L. (2024) · Journal of Applied Phycology · doi:10.1007/s10811-024-03258-2 · Cited by 7 · see citing works
Pharmaceutical and Biomedical Potential of Sulphated Polysaccharides from Algae
Morais, A. M. M. B., Alves, A. P. N. N., Kumla, D., & Morais, R. M. S. C. (2021) · Springer eBooks · doi:10.1007/978-3-030-35734-4_49-1 · Cited by 7 · see citing works
Jasińska, J. M., Kamińska, I., Chmiel, M. J., & Jamróz, E. (2023) · Biotechnology Journal · doi:10.1002/biot.202200455 · Cited by 7 · see citing works
Marine Fungal Metabolites: A Promising Source for Antibiofilm Compounds
Almutairi, F. A., & Edrada‐Ebel, R. (2025) · Molecules · doi:10.3390/molecules30214266 · Cited by 6 · see citing works
Vladkova, T. G., Staneva, A. D., Avramova, I. A., Ivanova, I., & Gospodinova, D. N. (2023) · Materials · doi:10.3390/ma16103651 · Cited by 6 · see citing works
Khamis, A. A., El-Keiy, M. M., El-Gamal, M. M. T., Saad‐Allah, K. M., & Salem, M. M. (2025) · Cell Biochemistry and Biophysics · doi:10.1007/s12013-025-01678-6 · Cited by 6 · see citing works
Rubiño, S., Aymerich, T., Peteiro, C., Bover‐Cid, S., & Hortos, M. (2023) · Journal of Applied Phycology · doi:10.1007/s10811-023-02923-2 · Cited by 6 · see citing works
Pereira, L. (2023) · Elsevier eBooks · doi:10.1016/b978-0-323-98819-3.00015-8 · Cited by 6 · see citing works
- Authors
- Jun, J.-Y., Jung, M.-J., Jeong, I.-H., Yamazaki, K., Kawai, Y., & Kim, B.-M.
- Affiliations
- Korea Food Research Institute (KR)
- Gangneung–Wonju National University (KR)
- Hokkaido University (JP)
- Corresponding author
- Kim, Byoung-Mok
- Venue
- Marine Drugs
- Publisher
- MDPI AG
- Published
- 2018-08-27
- Volume
- 16
- Issue
- 9
- Pages
- 301
- ISSN
- 1660-3397 (ISSN-L)
- PMC
- PMC6165115
- OpenAlex
- W2888717357
- Semantic Scholar
- a2ed968bf70c6c1b776ded6604621573641285fd
- Cited by
- 160
- References
- 28
- Citations by year
- 2026: 13 · 2025: 35 · 2024: 20 · 2023: 23 · 2022: 19 · 2021: 22 · 2020: 16 · 2019: 12
- FWCI
- 12.53
- Type
- journal article
- Language
- en
- Open access
- gold
- dx.doi.org
- Licence
- Locations
- Marine Drugs (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- Ministry of Science and ICT, South Korea
- Korea Food Research Institute
- Topics
- Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Seaweed-derived Bioactive Compounds
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bacterial biofilms and quorum sensing
- Health Sciences › Dentistry › Periodontics › Oral microbiology and periodontitis research
- Keywords
- sulfated polysaccharides, marine algae, fucoidan, Fucus vesiculosus, dental plaque, antibiofilm activity, antimicrobial activity, Streptococcus mutans, Streptococcus sobrinus, foodborne pathogens, minimum inhibitory concentration, molecular weight, heat treatment (food and biological processing), dental disease prevention
- MeSH terms
- Anti-Bacterial Agents, Dental Plaque, Drug Evaluation, Preclinical, Humans, Microbial Sensitivity Tests, Polysaccharides, Streptococcus mutans, Sulfuric Acid Esters, Streptococcus sobrinus, Biofilms, Fucus, Aquatic Organisms
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Claims read by deepseek-v4.1-flash under definition version 3 (extract-v3) on 2026-10-07.
Classified by jev-1.13-20260917 (gold-guideline-v0.2) on 2026-10-07.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
