Effect of sodium butyrate on the assembly, charge variants, and galactosylation of antibody produced in recombinant Chinese hamster ovary cells
Abstract
Sodium butyrate (NaBu) is known to increase the specific productivity of recombinant Chinese hamster ovary (rCHO) cells. To understand the effects of NaBu on the product quality, rCHO cells producing monoclonal antibody (Mab) were cultivated at various concentrations of NaBu (0 to 4 mM). NaBu increased correctly assembled Mab. In the absence of NaBu, the proportions of intact Mab (2H2L) and heavy chain dimer (2H) were 81 and 15 %. At 1 mM NaBu, the proportion of 2H2L increased to 93 %, whereas the proportion of 2H decreased to 2 %. No further increase in the proportion of 2H2L was obtained at a higher NaBu concentration. NaBu also affected the charge heterogeneity of Mab, which may affect the efficacy of Mab. The basic charge variants of Mabs increased with an increase in the NaBu concentration. In addition, NaBu affected the galactosylation of Mab negatively. Overall, the data obtained here show that NaBu used in rCHO cell cultures for improved Mab production affects certain quality aspects of Mab, in this case, the charge heterogeneity and galactosylation.
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In fed-batch cultures, the most common process strategies to arrest cell growth are reducing culture temperature from 37°C to 30-34°C or adding chemical agents such as sodium butyrate.
“In fed-batch cultures, the most common process strategies to arrest cell growth are by reducing the culture temperature from 37 • C to temperatures ranging from 30 • C to 34 • C, or through the addition of chemical agents such as sodium butyrate.[20]”
From Biomass specific perfusion rate as a control lever for the continuous manufacturing of biosimilar monoclonal antibodies from CHO cell cultures (2024) · cites this paper as [20]
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- Authors
- Hong, J. K., Lee, S. M., Kim, K.-Y., & Lee, G. M.
- Affiliations
- Korea Advanced Institute of Science and Technology (KR)
- Corresponding author
- Lee, Sang Min
- Lee, Gyun Min
- Venue
- Applied Microbiology and Biotechnology
- Published
- 2014-02-21
- Volume
- 98
- Issue
- 12
- Pages
- 5417-5425
- ISSN
- 0175-7598 (ISSN-L)
- 1432-0614
- OpenAlex
- W2010303718
- Cited by
- 46
- References
- 39
- Citations by year
- 2026: 3 · 2025: 1 · 2024: 8 · 2023: 1 · 2022: 4 · 2021: 5 · 2020: 2 · 2019: 2 · 2018: 8 · 2017: 7 · 2016: 2 · 2015: 3
- FWCI
- 1.64
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Applied Microbiology and Biotechnology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Ministry of Knowledge Economy
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Viral Infectious Diseases and Gene Expression in Insects
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein purification and stability
- Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Monoclonal and Polyclonal Antibodies Research
- Keywords
- sodium butyrate, monoclonal antibodies, antibody assembly, charge variants, galactosylation, antibody quality, product quality
- MeSH terms
- Animals, Antibodies, Monoclonal, Cricetulus, Galactose, Glycosylation, Cricetinae, Immunoglobulin Heavy Chains, Recombinant Proteins, CHO Cells, Protein Folding, Butyric Acid
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