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Cited by 27Microbial Metabolic Engineering and Bioproductionbiodegradable polymer synthesis and propertiesBacterial Genetics and Biotechnology

Synthetic biology toolkit of Ralstonia eutropha (Cupriavidus necator)

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Santolin, Lara, Riedel, Sebastian L., Brigham, Christopher J.·2024·Applied Microbiology and Biotechnology
DOI 10.1007/s00253-024-13284-2PMC PMC11362209
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Abstract

Synthetic biology encompasses many kinds of ideas and techniques with the common theme of creating something novel. The industrially relevant microorganism, Ralstonia eutropha (also known as Cupriavidus necator ), has long been a subject of metabolic engineering efforts to either enhance a product it naturally makes (polyhydroxyalkanoate) or produce novel bioproducts (e.g., biofuels and other small molecule compounds). Given the metabolic versatility of R. eutropha and the existence of multiple molecular genetic tools and techniques for the organism, development of a synthetic biology toolkit is underway. This toolkit will allow for novel, user-friendly design that can impart new capabilities to R. eutropha strains to be used for novel application. This article reviews the different synthetic biology techniques currently available for modifying and enhancing bioproduction in R. eutropha . Key points: • R. eutropha (C. necator) is a versatile organism that has been examined for many applications . • Synthetic biology is being used to design more powerful strains for bioproduction . • A diverse synthetic biology toolkit is being developed to enhance R. eutropha’s capabilities .

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  • Any future advancement of 5-G biofuels from the current conceptual stage is entirely contingent upon extensive and high-risk fundamental research and development across numerous areas, including advanced synthetic biology toolkits, breakthroughs in metabolic engineering, new reactor designs, efficient CO2 capture and delivery systems, robust LCA methodologies, and resolving challenges related to长期

    “Any future advancement from the current conceptual stage is entirely contingent upon extensive and high-risk fundamental research and development across numerous areas.These include significantly more advanced synthetic biology toolkits, breakthroughs in metabolic engineering for precise and efficient target molecule synthesis, entirely new reactor designs for these novel bioprocesses, highly”

    From Biofuels, E-Fuels, and Waste-Derived Fuels: Advances, Challenges, and Future Directions (2025) · cites this paper as [188]

    The “Synthetic biology of R. eutropha” section (page unavailable) says expression systems, genome-editing tools, and metabolic understanding are important for developing efficient biotechnological processes. “Application and products” (page unavailable) also identifies challenges in coupling CO2-converting microbes to electrochemical systems. The paper does not establish that 5-G biofuels are at a conceptual stage or that advancement is entirely contingent on high-risk research spanning new reactor designs, CO2 capture and delivery, LCA, and long-term challenges. (LLM, conf=0.84)

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Cited by

Sorted by citations

Authors
Santolin, L., Riedel, S. L., & Brigham, C. J.
Affiliations
  • Technische Universität Berlin (DE)
  • Berliner Hochschule für Technik (DE)
  • University of Massachusetts Dartmouth (US)
Corresponding author
  • Riedel, Sebastian L.
  • Brigham, Christopher J.
ORCID
Venue
Applied Microbiology and Biotechnology
Publisher
Springer Science and Business Media LLC
Published
2024-08-29
Volume
108
Issue
1
Pages
450-450
ISSN
  • 0175-7598 (ISSN-L)
  • 1432-0614
DOI
10.1007/s00253-024-13284-2
PMC
PMC11362209
OpenAlex
W4402005681
Cited by
27
References
2
Citations by year
2026: 11 · 2025: 14 · 2024: 2
FWCI
1.10
Type
journal article
Language
en
Open access
Licence
Locations
  • Applied Microbiology and Biotechnology (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
Funders
  • Bundesministerium für Bildung und Forschung (031B1339A)
  • Berliner Hochschule für Technik
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
  • Physical Sciences › Materials Science › Biomaterials › biodegradable polymer synthesis and properties
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
Keywords
Cupriavidus necator, synthetic biology toolkit, metabolic engineering, polyhydroxyalkanoates, bioproduction, biofuels, strain design, molecular genetic tools
MeSH terms
Cupriavidus necator, Polyhydroxyalkanoates, Biofuels, Synthetic Biology, Metabolic Engineering

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