Effective induction of anti-melanoma immunity following genetic vaccination with synthetic mRNA coding for the fusion protein EGFP.TRP2
Open accessAbstract
RNA-based genetic immunization represents an alternative novel strategy for antigen-specific cancer vaccines. In the present paper we investigate the use of synthetic messenger RNA in an experimental melanoma model. We show that gene gun-based immunization using synthetic RNA mediates gene expression in the epidermis and effectively induces antigen-specific cellular and humoral immunity in mice in vivo. Importantly, bombardment of the skin with RNA coding for the melanocytic self-antigen TRP2 linked to the immunogenic protein EGFP was associated with protection against experimentally induced B16 melanoma lung metastases and vitiligo-like fur depigmentation. Our results provide a scientific basis for clinical trials using synthetic mRNA encoding melanocytic antigens linked to immunogenic helper proteins for vaccination of patients with melanoma.
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In some reports, the poly(A) tail is attached enzymatically by terminal polyadenylation instead of being encoded in the template.
“In some reports, the poly(A) tail is attached enzymatically by terminal polyadenylation instead of being encoded in the template.13”
From Modification of antigen-encoding RNA increases stability, translational efficacy, and T-cell stimulatory capacity of dendritic cells (2006) · cites this paper as [13]
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- Authors
- Steitz, J., Britten, C. M., Wölfel, T., & Tüting, T.
- Affiliations
- University of Bonn (DE)
- Johannes Gutenberg University Mainz (DE)
- Corresponding author
- Tüting, Thomas
- Venue
- Cancer Immunology, Immunotherapy
- Published
- 2005-08-13
- Volume
- 55
- Issue
- 3
- Pages
- 246-253
- ISSN
- 0340-7004 (ISSN-L)
- 1432-0851
- PMC
- PMC11030217
- OpenAlex
- W1971250275
- Cited by
- 65
- References
- 1
- Citations by year
- 2025: 1 · 2024: 4 · 2022: 7 · 2021: 1 · 2020: 12 · 2019: 2 · 2018: 6 · 2017: 1 · 2016: 2 · 2015: 4 · 2014: 5 · 2013: 1 · 2012: 1
- FWCI
- 0.89
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Cancer Immunology Immunotherapy (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Immunology and Microbiology › Immunology › Immunotherapy and Immune Responses
- Health Sciences › Medicine › Oncology › Cancer Immunotherapy and Biomarkers
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
- Keywords
- melanoma, synthetic mRNA, genetic immunization, TRP-2, EGFP fusion protein, gene gun immunization, antigen-specific immunity, B16 melanoma, lung metastasis, vitiligo-like depigmentation, cancer vaccines
- MeSH terms
- Animals, beta-Galactosidase, Enzyme-Linked Immunosorbent Assay, Melanoma, Experimental, Membrane Proteins, Mice, Inbred C57BL, Recombinant Fusion Proteins, RNA, Messenger, Vitiligo, Gene Expression, Biolistics, Cancer Vaccines, Green Fluorescent Proteins, Mice
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