Induction of HIV-1 gag specific immune responses by cationic micelles mediated delivery of gag mRNA
Open accessAbstract
In recent years, mRNA-based vaccines have emerged to be a great alternative to DNA-based vaccines due to the safety of not inserting into host genome. However, mRNA molecules are single-stranded nucleic acids that are vulnerable under RNase existing in human skin and tissues. In this study, a self-assembled cationic nanomicelles based on polyethyleneimine-stearic acid (PSA) copolymer were developed to delivery HIV-1 gag encoding mRNA to dendritic cells and BALB/c mice. We evaluated the transfection efficiency and cell uptake efficiency of naked EGFP mRNA, PSA, PEI-2k and PEI-25k nanoparticles format on DC2.4 cell lines. Immune responses after sub-cutaneous administration of gag mRNA to BALB/c mice were notably induced by PSA as compared with naked gag mRNA. We found the PSA/mRNA nanomicelles were potent systems that can effectively deliver mRNA and induce antigen-specific immune response, stimulating various new vaccine strategies using mRNA.
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Several preclinical studies have delivered mRNA vaccines encoding HIV proteins using multiple delivery vehicles, but they have had varied success.
“Several preclinical studies have delivered mRNA vaccines encoding HIV proteins using multiple delivery vehicles, including cationic nanoemulsions109,161, DOTAP/DOPE liposomes162, polymers84,163,164 and ionizable LNPs165,166, but they have had varied success.”
From mRNA vaccines for infectious diseases: principles, delivery and clinical translation (2021) · cites this paper as [164]
Novel antigens are necessary to effectively target HIV in addition to potent delivery vehicles.
“These studies suggest that novel antigens are necessary to effectively target HIV in addition to potent delivery vehicles.”
From mRNA vaccines for infectious diseases: principles, delivery and clinical translation (2021) · cites this paper as [164]
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- Authors
- Zhao, M., Li, M., Zhang, Z., Gong, T., & Sun, X.
- Affiliations
- Sichuan University (CN)
- Corresponding author
- Sun, Xun
- Venue
- Drug Delivery
- Publisher
- Informa UK Limited
- Published
- 2015-05-29
- Volume
- 23
- Issue
- 7
- Pages
- 2596-2607
- ISSN
- 1071-7544 (ISSN-L)
- 1521-0464
- OpenAlex
- W2150490625
- Cited by
- 132
- References
- 43
- Citations by year
- 2026: 4 · 2025: 10 · 2024: 15 · 2023: 19 · 2022: 14 · 2021: 25 · 2020: 20 · 2019: 9 · 2018: 10 · 2017: 2 · 2016: 3 · 2015: 1
- FWCI
- 2.31
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Drug Delivery (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Interference and Gene Delivery
- Life Sciences › Immunology and Microbiology › Immunology › Immunotherapy and Immune Responses
- Physical Sciences › Materials Science › Biomaterials › Nanoparticle-Based Drug Delivery
- Keywords
- HIV-1 Gag, mRNA vaccines, dendritic cells, mRNA delivery, antigen-specific immune response, transfection efficiency, cellular uptake, subcutaneous immunization
- MeSH terms
- Animals, Cations, Dendritic Cells, Humans, Mice, Inbred BALB C, Micelles, Ribonucleases, RNA, Messenger, Stearic Acids, Transfection, Vaccines, HIV-1, Genes, gag, Mice
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