Two pentatricopeptide repeat domain proteins are required for the synthesis of respiratory complex I
Abstract
In this study pentatricopeptide repeat (PPR) proteins in filamentous ascomycetes are identified and functionally characterized. PPR proteins, which have in common a degenerated 35 amino acid motif often arranged in multiple tandems, are known to be implicated in various steps of RNA metabolism in mitochondria and chloroplasts. In filamentous ascomycetes we identified a common set of nine PPR proteins. For seven of these proteins, which were not yet characterized, knockout mutants of Neurospora crassa were analyzed. The knockout of three genes appeared to be lethal while four mutants showed different degrees of alterations in respiratory chain complexes. Two mutants are specifically affected in the assembly of a functional complex I while the other enzymes of the respiratory chain are present. Both mutants demonstrate the presence of a peripheral arm and the absence of a detectable membrane arm. Analysis of the mitochondrial RNA revealed distinct alterations of the transcript patterns for certain complex I subunits. Synthesis and/or stability of the transcript for ND2-ND3 is grossly impaired in one mutant while in the other mutant splicing of the transcript for ND1-ND4 is hampered. Our analysis provides the basis for a comprehensive characterization of PPR proteins in filamentous ascomycetes.
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Ncu20711 is a PPR protein required for ND1-ND4 splicing in N. crassa.
“An example of such regulation is provided by Ncu20711, a PPR protein required for ND1–ND4 splicing in N. crassa (Solotoff et al. 2015).”
From <i>Candida albicans</i> PPR proteins are required for the expression of respiratory Complex I subunits (2024) · cites this paper as Solotoff et al. 2015
CaPpr7 shows functional similarity to ScMrx1p, SpPpr10p, and Ncu08674 of N. crassa.
“Although CaPpr7 is not clearly orthologous to any known fungal PPR proteins, it shows some functional similarity to ScMrx1p (Kehrein et al. 2015), SpPpr10p (Wang et al. 2017), and Ncu08674 of N. crassa (Solotoff et al. 2015).”
From <i>Candida albicans</i> PPR proteins are required for the expression of respiratory Complex I subunits (2024) · cites this paper as Solotoff et al. 2015
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- Authors
- Solotoff, V., Moseler, R., & Schulte, U.
- Affiliations
- Heinrich Heine University Düsseldorf (DE)
- Corresponding author
- Schulte, U.
- Venue
- Current Genetics
- Published
- 2014-08-10
- Volume
- 61
- Issue
- 1
- Pages
- 19-29
- ISSN
- 0172-8083 (ISSN-L)
- 1432-0983
- OpenAlex
- W2016856950
- Cited by
- 7
- References
- 47
- Citations by year
- 2024: 1 · 2020: 1 · 2017: 2 · 2016: 2 · 2015: 1
- FWCI
- 0.68
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- Locations
- Current Genetics (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
- Keywords
- pentatricopeptide repeat proteins, mitochondrial complex I, mitochondrial RNA metabolism, Neurospora crassa, knockout mutants, transcript splicing, filamentous ascomycetes
- MeSH terms
- Ascomycota, Mitochondria, Mutation, Transcription, Genetic, Protein Subunits, Mitochondrial Proteins, Electron Transport Complex I, Genes, Mitochondrial, Protein Interaction Domains and Motifs, Gene Knockdown Techniques
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