Cited by 7Mitochondrial Function and PathologyFungal and yeast genetics researchRNA Research and Splicing

Two pentatricopeptide repeat domain proteins are required for the synthesis of respiratory complex I

Solotoff, V., Moseler, R., Schulte, U.·2015·Current Genetics

Abstract

In this study pentatricopeptide repeat (PPR) proteins in filamentous ascomycetes are identified and functionally characterized. PPR proteins, which have in common a degenerated 35 amino acid motif often arranged in multiple tandems, are known to be implicated in various steps of RNA metabolism in mitochondria and chloroplasts. In filamentous ascomycetes we identified a common set of nine PPR proteins. For seven of these proteins, which were not yet characterized, knockout mutants of Neurospora crassa were analyzed. The knockout of three genes appeared to be lethal while four mutants showed different degrees of alterations in respiratory chain complexes. Two mutants are specifically affected in the assembly of a functional complex I while the other enzymes of the respiratory chain are present. Both mutants demonstrate the presence of a peripheral arm and the absence of a detectable membrane arm. Analysis of the mitochondrial RNA revealed distinct alterations of the transcript patterns for certain complex I subunits. Synthesis and/or stability of the transcript for ND2-ND3 is grossly impaired in one mutant while in the other mutant splicing of the transcript for ND1-ND4 is hampered. Our analysis provides the basis for a comprehensive characterization of PPR proteins in filamentous ascomycetes.

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Crossref · 10.1007/s00294-014-0441-2

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Authors
Solotoff, V., Moseler, R., & Schulte, U.
Affiliations
Heinrich Heine University Düsseldorf (DE)
Corresponding author
Schulte, U.
Venue
Current Genetics
Published
2014-08-10
Volume
61
Issue
1
Pages
19-29
ISSN
  • 0172-8083 (ISSN-L)
  • 1432-0983
OpenAlex
W2016856950
Cited by
7
References
47
Citations by year
2024: 1 · 2020: 1 · 2017: 2 · 2016: 2 · 2015: 1
FWCI
0.68
Type
journal article
Language
en
Open access
closed
Locations
  • Current Genetics (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
Keywords
pentatricopeptide repeat proteins, mitochondrial complex I, mitochondrial RNA metabolism, Neurospora crassa, knockout mutants, transcript splicing, filamentous ascomycetes
MeSH terms
Ascomycota, Mitochondria, Mutation, Transcription, Genetic, Protein Subunits, Mitochondrial Proteins, Electron Transport Complex I, Genes, Mitochondrial, Protein Interaction Domains and Motifs, Gene Knockdown Techniques

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