MicroRNA expression profiles in conventional and micropropagated strawberry (Fragaria × ananassa Duch.) plants
Abstract
MicroRNAs (miRNAs) are a class of small non-coding RNAs which play a critical role in plant growth and development. To detect strawberry miRNAs and discover the expression difference between conventional and micropropagated strawberry plants, we carried out the detection and quantification of strawberry miRNAs by microarray. The main findings were that 74 miRNAs were checked in strawberry plants and four miRNA genes displayed clear expression difference between conventional and micropropagated strawberry plants, including two up-regulated genes (miR535 and miR390) and two down-regulated genes (miR169a and miR169d). The ratios of conventionally propagated strawberry plant/micropropagated strawberry plant for miR535, miR390, miR169a and miR169d were 2.6884, 2.2673, 0.2496 and 0.3814, respectively. Quantitative reverse transcription polymerase chain reaction applied to the two up-regulated genes (miR535 and miR390) validated the microarray result. This is the first report on differential expression of miRNAs in conventional and micropropagated plants.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In strawberry, changes in the levels of miR169a and miR390 were associated with altered phenotypes in tissue-cultured plants, including altered stomatal aperture size and timing of flower bud differentiation.
“The changes in the levels of miR169a and miR390 were associated with altered phenotypes in tissue-cultured strawberry plants (altered stomatal aperture size and timing of flower bud differentiation) [130].”
From Somaclonal Variation—Advantage or Disadvantage in Micropropagation of the Medicinal Plants (2023) · cites this paper as [130]
During micropropagation, small RNA pathways can become disrupted, leading to misregulation of critical genes and emergence of somaclonal variation.
“During micropropagation, these small RNA pathways can become disrupted, leading to the misregulation of critical genes and, consequently, the emergence of somaclonal [63] (Figure 3).”
From Somaclonal Variation and Clonal Fidelity in Commercial Micropropagation: Challenges and Perspectives (2025) · cites this paper as [63]
No citation lists for this paper
No citation lists for this paper yet — checked again daily.
References 99 · Cited by 46
- Authors
- Li, H., Zhang, Z., Huang, F., Chang, L., & Ma, Y.
- Affiliations
- Shenyang Agricultural University (CN)
- Corresponding author
- Zhang, Zhihong
- Venue
- Plant Cell Reports
- Published
- 2009-03-10
- Volume
- 28
- Issue
- 6
- Pages
- 891-902
- ISSN
- 0721-7714 (ISSN-L)
- 1432-203X
- OpenAlex
- W2012391394
- Cited by
- 46
- References
- 99
- Citations by year
- 2025: 2 · 2024: 1 · 2023: 4 · 2022: 4 · 2021: 4 · 2020: 5 · 2019: 2 · 2016: 5 · 2015: 2 · 2014: 6 · 2013: 1 · 2012: 6
- FWCI
- 4.04
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Plant Cell Reports (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Natural Science Foundation of China
- Program for New Century Excellent Talents in University
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant tissue culture and regeneration
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Berry genetics and cultivation research
- Keywords
- microRNA, strawberry, micropropagation, miRNA expression profiling, miR390, differential gene expression, plant growth and development
- MeSH terms
- Gene Expression Regulation, Plant, RNA, Plant, Oligonucleotide Array Sequence Analysis, Gene Expression Profiling, Fragaria, MicroRNAs
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
