CoChat
Sign Up
Cited by 67Wound Healing and TreatmentsInflammation biomarkers and pathwaysForensic Entomology and Diptera Studies

Simultaneous detection of eight cytokines in human dermal wounds with a multiplex bead-based immunoassay for wound age estimation

Takamiya, Masataka, Fujita, Sachiko, Saigusa, Kiyoshi, Aoki, Yasuhiro·2008·International Journal of Legal Medicine
DOI 10.1007/s00414-007-0183-5
Open this paper in CoChat — free account

Abstract

We performed quantification of IL 2, IL 4, IL 6, IL 8, IL 10, GM-CSF, IFN gamma, and TNF alpha in human dermal wounds for wound age estimation. The proliferation of dermal cells and infiltration of inflammatory cells were also analyzed. Neutrophils and macrophages were detected from 2 h post-injury, and strong infiltrations were seen at 33-49 h. T and B lymphocytes also infiltrated simultaneously from 71 h. Strong proliferation of fibroblasts were shown from 246 h, and thickening of the epidermis from 71 h. IL 10, GM-CSF, IFNgamma, and TNF alpha increased from the early phase of dermal wound healing, IL 6 exclusively in the middle phase, IL 2, IL 4, and IL 8 from the middle phase to the late phase. Among the cytokines analyzed in the present study, IL 6, IL 8, IFNgamma, and TNF alpha were strongly expressed. Results of the present study suggest that multiplex cytokine analysis at the wound site can be useful for wound age estimation. In addition, multiplex data obtained from the same sample with a single method would demonstrate more accurate interactions of cytokines during dermal wound healing. Although the present study was oriented to practical forensic pathology, the data obtained would be informative for various fields of medicine.

Integrity signals

Registry

Crossref · 10.1007/s00414-007-0183-5

Cites

37 of 37 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • IL-4 gene expression is elevated from 1 to 7 days in rat skin wounds, and protein expression is elevated between 12 h and 10 days in human skin wounds.

    “However, IL-4 gene expression is elevated from 1 to 7 days in rat skin wounds [56], and protein expression is elevated between 12 h and 10 days in human skin wounds (peak ϳ12 pg/mg protein in injured skin vs. nearly undetectable in uninjured) [58]; IL-4 protein is present at similar levels in infarcted rat myocardium [72].”

    From Macrophage phenotypes during tissue repair (2013) · cites this paper as [58]

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Related

Authors
Takamiya, M., Fujita, S., Saigusa, K., & Aoki, Y.
Affiliations
Iwate Medical University (JP)
Corresponding author
Takamiya, Masataka
ORCID
Aoki, Yasuhiro
Venue
International Journal of Legal Medicine
Publisher
Springer Science and Business Media LLC
Published
2007-08-28
Volume
122
Issue
2
Pages
143-148
ISSN
  • 0044-3433 (ISSN-L)
  • 0937-9827
  • 1437-1596
DOI
10.1007/s00414-007-0183-5
OpenAlex
W1967958218
Cited by
67
References
37
Citations by year
2026: 1 · 2024: 3 · 2023: 2 · 2022: 9 · 2021: 2 · 2020: 3 · 2019: 1 · 2018: 2 · 2017: 10 · 2016: 6 · 2015: 2 · 2014: 2 · 2013: 6 · 2012: 1
FWCI
4.69
Type
journal article
Language
en
Open access
closed
Licence
www.springer.com/tdm (tdm)
Locations
  • International Journal of Legal Medicine (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Rehabilitation › Wound Healing and Treatments
  • Life Sciences › Immunology and Microbiology › Immunology › Inflammation biomarkers and pathways
  • Life Sciences › Agricultural and Biological Sciences › Insect Science › Forensic Entomology and Diptera Studies
Keywords
wound age estimation, skin wounds, cytokines, multiplex bead immunoassay, wound healing, interleukin-6, interleukin-8, IL-10, interferon-gamma, TNF-α, GM-CSF, interleukin-2, IL-4, inflammatory cell infiltration, fibroblast proliferation, epidermal thickening, forensic pathology
MeSH terms
B-Lymphocytes, Female, Fibroblasts, Humans, Immunohistochemistry, Macrophages, Male, Neutrophils, Skin, T-Lymphocytes, Wound Healing, Cytokines, Cell Proliferation, Forensic Pathology

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review