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Cited by 83Nuclear Structure and FunctionGenomics and Chromatin DynamicsChromosomal and Genetic Variations

Nucleolus and chromatin

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Schöfer, Christian, Weipoltshammer, Klara·2018·Histochemistry and Cell Biology
DOI 10.1007/s00418-018-1696-3PMC PMC6096769
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Abstract

The nucleolus as site of ribosome biogenesis holds a pivotal role in cell metabolism. It is composed of ribosomal DNA (rDNA), which is present as tandem arrays located in nucleolus organizer regions (NORs). In interphase cells, rDNA can be found inside and adjacent to nucleoli and the location is indicative for transcriptional activity of ribosomal genes-inactive rDNA (outside) versus active one (inside). Moreover, the nucleolus itself acts as a spatial organizer of non-nucleolar chromatin. Microscopy-based approaches offer the possibility to explore the spatially distinct localization of the different DNA populations in relation to the nucleolar structure. Recent technical developments in microscopy and preparatory methods may further our understanding of the functional architecture of nucleoli. This review will attempt to summarize the current understanding of mammalian nucleolar chromatin organization as seen from a microscopist's perspective.

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Cited by

Sorted by citations

Authors
Schöfer, C., & Weipoltshammer, K.
Affiliations
Medical University of Vienna (AT)
Corresponding author
Schöfer, Christian
ORCID
Venue
Histochemistry and Cell Biology
Publisher
Springer Science and Business Media LLC
Published
2018-07-25
Volume
150
Issue
3
Pages
209-225
ISSN
  • 0948-6143 (ISSN-L)
  • 1432-119X
DOI
10.1007/s00418-018-1696-3
PMC
PMC6096769
OpenAlex
W2884385526
Cited by
83
References
5
Citations by year
2026: 5 · 2025: 5 · 2024: 13 · 2023: 9 · 2022: 13 · 2021: 12 · 2020: 12 · 2019: 12 · 2018: 1 · 2017: 1
FWCI
0.91
Type
journal article
Language
en
Open access
Licence
Locations
  • Histochemistry and Cell Biology (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
Funders
Austrian Science Fund FWF and Herzfelder´sche Familienstiftung (P30642-b28)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nuclear Structure and Function
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Chromosomal and Genetic Variations
Keywords
nucleolus, nucleolar chromatin, ribosome biogenesis, ribosomal DNA, nucleolar organizer regions, rDNA transcription, mammalian cells
MeSH terms
Animals, Cell Nucleus, Chromatin, Humans, Microscopy

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