Cited by 53Genomics and Chromatin DynamicsNuclear Structure and FunctionChromosomal and Genetic Variations

The CCCTC-binding factor (CTCF) of drosophila contributes to the regulation of the ribosomal DNA and nucleolar stability

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Guerrero, Paola A., Maggert, Keith A.·2011·PLoS ONE

Abstract

In the repeat array of ribosomal DNA (rDNA), only about half of the genes are actively transcribed while the others are silenced. In arthropods, transposable elements interrupt a subset of genes, often inactivating transcription of those genes. Little is known about the establishment or separation of juxtaposed active and inactive chromatin domains, or preferential inactivation of transposable element interrupted genes, despite identity in promoter sequences. CTCF is a sequence-specific DNA binding protein which is thought to act as a transcriptional repressor, block enhancer-promoter communication, and delimit juxtaposed domains of active and inactive chromatin; one or more of these activities might contribute to the regulation of this repeated gene cluster. In support of this hypothesis, we show that the Drosophila nucleolus contains CTCF, which is bound to transposable element sequences within the rDNA. Reduction in CTCF gene activity results in nucleolar fragmentation and reduced rDNA silencing, as does disruption of poly-ADP-ribosylation thought to be necessary for CTCF nucleolar localization. Our data establish a role for CTCF as a component necessary for proper control of transposable element-laden rDNA transcription and nucleolar stability.

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Authors
Guerrero, P. A., & Maggert, K. A.
Affiliations
Texas A&M University (US)
Corresponding author
Maggert, Keith A.
Venue
PLoS ONE
Published
2011-01-20
Volume
6
Issue
1
Pages
e16401
ISSN
1932-6203 (ISSN-L)
OpenAlex
W1993463805
Cited by
51
References
60
Citations by year
2026: 1 · 2025: 2 · 2024: 3 · 2023: 2 · 2022: 2 · 2021: 3 · 2020: 1 · 2019: 3 · 2018: 4 · 2017: 3 · 2016: 2 · 2015: 5 · 2014: 7 · 2013: 3 · 2012: 6
FWCI
2.60
Type
journal article
Language
en
Open access
Locations
  • PLoS ONE (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
  • OakTrust (Texas A&M University Libraries) (submittedVersion)
Funders
  • National Institutes of Health
  • National Institute of General Medical Sciences (R01GM076092)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nuclear Structure and Function
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Chromosomal and Genetic Variations
Keywords
CTCF, ribosomal DNA, rDNA silencing, nucleolar fragmentation, transposable elements, chromatin domains, poly(ADP-ribosyl)ation, nucleolar localization, Drosophila, transcriptional repression
MeSH terms
CCCTC-Binding Factor, Animals, Cell Nucleolus, DNA Transposable Elements, DNA, Ribosomal, Drosophila melanogaster, Protein Binding, Repressor Proteins, Gene Silencing

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