The CCCTC-binding factor (CTCF) of drosophila contributes to the regulation of the ribosomal DNA and nucleolar stability
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In the repeat array of ribosomal DNA (rDNA), only about half of the genes are actively transcribed while the others are silenced. In arthropods, transposable elements interrupt a subset of genes, often inactivating transcription of those genes. Little is known about the establishment or separation of juxtaposed active and inactive chromatin domains, or preferential inactivation of transposable element interrupted genes, despite identity in promoter sequences. CTCF is a sequence-specific DNA binding protein which is thought to act as a transcriptional repressor, block enhancer-promoter communication, and delimit juxtaposed domains of active and inactive chromatin; one or more of these activities might contribute to the regulation of this repeated gene cluster. In support of this hypothesis, we show that the Drosophila nucleolus contains CTCF, which is bound to transposable element sequences within the rDNA. Reduction in CTCF gene activity results in nucleolar fragmentation and reduced rDNA silencing, as does disruption of poly-ADP-ribosylation thought to be necessary for CTCF nucleolar localization. Our data establish a role for CTCF as a component necessary for proper control of transposable element-laden rDNA transcription and nucleolar stability.
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The possible role of spatial interactions in transcriptional selection of rDNA repeats is supported by the R2 upregulation upon mutation of the chromatin architectural protein CTCF.
“The possible role of spatial interactions in transcriptional selection of rDNA repeats is supported by the R2 upregulation upon mutation of the chromatin architectural protein CTCF (53).”
From Impaired function of rDNA transcription initiation machinery leads to derepression of ribosomal genes with insertions of R2 retrotransposon (2022) · cites this paper as [53]
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“The possible role of spatial interactions in transcriptional selection of rDNA repeats is supported by the R2 upregulation upon mutation of the chromatin architectural protein CTCF (53).”
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- Authors
- Guerrero, P. A., & Maggert, K. A.
- Affiliations
- Texas A&M University (US)
- Corresponding author
- Maggert, Keith A.
- Venue
- PLoS ONE
- Publisher
- Public Library of Science (PLoS)
- Published
- 2011-01-20
- Volume
- 6
- Issue
- 1
- Pages
- e16401
- ISSN
- 1932-6203 (ISSN-L)
- PMC
- PMC3024428
- OpenAlex
- W1993463805
- Cited by
- 51
- References
- 60
- Citations by year
- 2026: 1 · 2025: 2 · 2024: 3 · 2023: 2 · 2022: 2 · 2021: 3 · 2020: 1 · 2019: 3 · 2018: 4 · 2017: 3 · 2016: 2 · 2015: 5 · 2014: 7 · 2013: 3 · 2012: 6
- FWCI
- 2.60
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- PLoS ONE (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- OakTrust (Texas A&M University Libraries) (submittedVersion)
- Funders
- National Institutes of Health
- National Institute of General Medical Sciences (R01GM076092)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nuclear Structure and Function
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Chromosomal and Genetic Variations
- Keywords
- CTCF, ribosomal DNA, rDNA silencing, nucleolar fragmentation, transposable elements, chromatin domains, poly(ADP-ribosyl)ation, nucleolar localization, Drosophila, transcriptional repression
- MeSH terms
- CCCTC-Binding Factor, Animals, Cell Nucleolus, DNA Transposable Elements, DNA, Ribosomal, Drosophila melanogaster, Protein Binding, Repressor Proteins, Gene Silencing
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