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Cited by 109Adipokines, Inflammation, and Metabolic DiseasesAdipose Tissue and MetabolismProtease and Inhibitor Mechanisms

The DOI matches its Crossref record; 53 of the 53 references it cites were found in a registry or an index.

Microarray analysis identifies matrix metalloproteinases (MMPs) as key genes whose expression is up-regulated in human adipocytes by macrophage-conditioned medium

O’Hara, Adrian, Lim, Fei-Ling, Mazzatti, Dawn J., Trayhurn, Paul·2009·Pflügers Archiv - European Journal of Physiology
DOI 10.1007/s00424-009-0693-8
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Abstract

White adipose tissue exhibits inflammation as tissue mass expands in obesity, involving macrophage infiltration and a direct inflammatory response by adipocytes. DNA microarrays and conditioned medium have been used to examine the effects of macrophages on global gene expression in human adipocytes. SGBS adipocytes, differentiated in culture, were treated with macrophage-conditioned medium (U937 cells) for 4 or 24 h; control cells received unconditioned medium. Agilent arrays comprising 44,000 probes were used to analyse gene expression. Microarray analysis identified 1,088 genes differentially expressed in response to the conditioned medium at both 4 and 24 h (754 up-regulated, 334 down-regulated at 24 h); these included genes associated with inflammation and macrophage infiltration. A cluster of matrix metalloproteinase genes were highly up-regulated at both time-points, including MMP1, MMP3, MMP9, MMP10, MMP12 and MMP19. At 4 and 24 h, MMP1 was the most highly up-regulated gene (>2,400-fold increase in mRNA at 24 h). ELISA measurements indicated that substantial quantities of MMP1 and MMP3 were released from adipocytes incubated with conditioned medium, with little release by control adipocytes. Treatment with TNFalpha induced substantial increases in MMP1 (>100-fold) and MMP3 (27-fold) mRNA level and MMP1 and MMP3 release in adipocytes, suggesting that this cytokine could contribute to the stimulation of MMP expression by macrophages. In conclusion, macrophage-secreted factors induce a major inflammatory response in human adipocytes, with expression of MMP family members being strongly up-regulated. The induction of MMP1 and other MMPs suggests that macrophages stimulate tissue remodelling during adipose tissue expansion in obesity.

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Metadata

Authors
O’Hara, A., Lim, F.-L., Mazzatti, D. J., & Trayhurn, P.
Affiliations
  • Unilever (United Kingdom) (GB)
  • University of Liverpool (GB)
Corresponding author
Trayhurn, Paul
ORCID
Venue
Pflügers Archiv - European Journal of Physiology
Publisher
Springer Science and Business Media LLC
Published
2009-07-09
Volume
458
Issue
6
Pages
1103-1114
ISSN
  • 0031-6768 (ISSN-L)
  • 1432-2013
DOI
10.1007/s00424-009-0693-8
OpenAlex
W2080442929
Cited by
108
References
53
Citations by year
2026: 3 · 2025: 4 · 2024: 2 · 2023: 5 · 2022: 6 · 2021: 5 · 2020: 6 · 2019: 2 · 2018: 5 · 2017: 5 · 2016: 9 · 2015: 8 · 2014: 6 · 2013: 8 · 2012: 16
FWCI
7.59
Type
journal article
Language
en
Open access
closed
Licence
www.springer.com/tdm (tdm)
Locations
  • Pflügers Archiv - European Journal of Physiology (publishedVersion)
  • PubMed (publishedVersion)
  • CLOK (University of Central Lancashire) (submittedVersion)
Funders
Biotechnology and Biological Sciences Research Council
Topics
  • Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Adipokines, Inflammation, and Metabolic Diseases
  • Health Sciences › Medicine › Physiology (medical) › Adipose Tissue and Metabolism
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Protease and Inhibitor Mechanisms
Keywords
matrix metalloproteinases, adipocytes, macrophage-conditioned medium, obesity, adipose tissue inflammation, macrophage infiltration, MMP-1, MMP-3, MMP-9, MMP-12, MMP-10, MMP-19, TNF-α, gene expression, tissue remodeling, SGBS cells
MeSH terms
Humans, Inflammation, Macrophages, Tumor Necrosis Factor-alpha, Up-Regulation, Culture Media, Conditioned, Adipocytes, Matrix Metalloproteinase 3, U937 Cells, Oligonucleotide Array Sequence Analysis, Matrix Metalloproteinase 1, Matrix Metalloproteinases

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