Effects of hydroxyl radicals on K ATP channels in guinea-pig ventricular myocytes
Abstract
We studied the effects of oxygen free radicals on the ATP-sensitive potassium channel (KATP channel) of guinea-pig ventricular myocytes. Single KATP channel currents were recorded from inside-out patches in the presence of symmetrical K+ concentrations (140 mM in both bath and pipette solutions). Reaction of xanthine oxidase (0.1 U/ml) on hypoxanthine (0.5 mM) produced superoxide anions (.O2-) and hydrogen peroxide (H2O2). Exposure of the patch membrane to.O2- and H2O2 increased the opening of KATP channels, but this activation was prevented by adding 1 microM glibenclamide to the bath solution. In the presence of ferric iron (Fe3+: 0.1 mM), the same procedure produced hydroxyl radicals (.OH) via the iron-catalysed Haber-Weiss reaction.OH also activated KATP channels; however, this activation could not be prevented by, even very high concentrations of glibenclamide (10 microM). These different effects of glibenclamide suggest that the mode of action of these oxygen free radicals on KATP channels is different and that.OH is more potent than.O2-/H2O2 in activating KATP channels in the heart.
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During early ischemia in isolated hearts, ROS increase along with mitochondrial [Ca2+] and the redox state (NADH).
“During early ischemia in isolated hearts, ROS increase along with mitochondrial [Ca2+] and the redox state (NADH), as shown in Fig. 17 (5, 70, 298)”
From Mitochondrial reactive oxygen species production in excitable cells: Modulators of mitochondrial and cell function (2009) · cites this paper as [298]
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Stowe, D. F., & Camara, A. K. S. (2009) · Antioxidants & Redox Signaling · doi:10.1089/ars.2008.2331 · Cited by 471 · see citing works
- Authors
- Tokube, K., Kiyosue, T., & Arita, M.
- ORCID
- Arita, M.
- Venue
- Pfl�gers Archiv European Journal of Physiology
- Published
- 1998-11-19
- Volume
- 437
- Issue
- 1
- Pages
- 155-157
- ISSN
- 0031-6768 (ISSN-L)
- 1432-2013
- OpenAlex
- W2037854364
- Cited by
- 51
- References
- 7
- Citations by year
- 2023: 1 · 2020: 1 · 2018: 2 · 2016: 1 · 2015: 3 · 2014: 2 · 2013: 1 · 2012: 2
- FWCI
- 1.05
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Pflügers Archiv - European Journal of Physiology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ion channel regulation and function
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
- Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Cardiac electrophysiology and arrhythmias
- Keywords
- hydroxyl radicals, ATP-sensitive potassium channels, oxygen free radicals, superoxide, hydrogen peroxide, xanthine oxidase, glibenclamide, ventricular myocytes, cardiac ion channels, oxidative stress
- MeSH terms
- Adenosine Triphosphate, Animals, Ferric Compounds, Glyburide, Guinea Pigs, Heart, Heart Ventricles, Hydrogen Peroxide, Superoxides, Xanthine Oxidase, Potassium Channels, Ventricular Function, Hydroxyl Radical, Patch-Clamp Techniques, Hypoxanthine
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