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Cited by 59Postharvest Quality and Shelf Life ManagementFood composition and propertiesBanana Cultivation and Research

The DOI matches its Crossref record; 28 of the 28 references it cites were found in a registry or an index.

Activity and expression of banana starch phosphorylases during fruit development and ripening

da Mota, Renata, Cordenunsi, Beatriz, do Nascimento, João, Purgatto, Eduardo, Rosseto, Maria, Lajolo, Franco·2002·Planta
DOI 10.1007/s00425-002-0858-6
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Abstract

Two main forms of starch phosphorylase (EC 2.4.1.1) were identified and purified from banana (Musa acuminata Colla. cv. Nanicão) fruit. One of them, designated phosphorylase I, had a native molecular weight of 155 kDa and subunit of 90 kDa, a high affinity towards branched glucans and an isoelectric point around 5.0. The other, phosphorylase II, eluted at a higher salt concentration from the anion exchanger, had a low affinity towards branched glucans, a native molecular weight of 290 kDa and subunit of 112 kDa. Kinetic studies showed that both forms had typical hyperbolic curves for orthophosphate (Pi) and glucose-1-phosphate, and that they could not react with substrates with a blocked reducing end or alpha-1,6 glucosidic bonds. Antibodies prepared against the purified type-II form and cross-reacting with the type-I form showed that there was an increase in protein content during development and ripening of the fruit. The changes in protein level were parallel to those of phosphorylase activity, in both the phosphorolytic and synthetic directions. Considering the kinetics, indicating that starch phosphorylases are not under allosteric control, it can be argued that protein synthesis makes a contribution to regulating phosphorylase activity in banana fruit and that hormones, like gibberellic acid and indole-3-acetic acid, may play a regulating role. For the first time, starch phosphorylases isoforms were detected as starch-granule-associated proteins by immunostaining of SDS-PAGE gels.

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Crossref · 10.1007/s00425-002-0858-6

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28 of 28 references verified0 citation errors · 0 not checkable

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  • Da Mota et al. extracted two different forms of phosphorylase from unripe banana (Musa acuminata Colla. cv. Nanicão) fruits-phosphorylase I and phosphorylase II.

    “Da Mota et al. [59] extracted two different forms of phosphorylase from unripe banana ( Musa acuminata Colla. cv. Nanic ã o) fruits-phosphorylase I and phosphorylase II.”

    From Potential Valorization of Banana Production Waste in Developing Countries: Bio-Engineering Aspects (2024) · cites this paper as [59]

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Metadata

Authors
da Mota, R., Cordenunsi, B., do Nascimento, J., Purgatto, E., Rosseto, M., & Lajolo, F.
Affiliations
Universidade de São Paulo (BR)
Corresponding author
Lajolo, Franco
ORCID
Venue
Planta
Publisher
Springer Science and Business Media LLC
Published
2002-12-01
Volume
216
Issue
2
Pages
325-333
ISSN
0032-0935 (ISSN-L)
DOI
10.1007/s00425-002-0858-6
OpenAlex
W2070643927
Cited by
59
References
28
Citations by year
2024: 4 · 2023: 1 · 2022: 1 · 2021: 2 · 2020: 1 · 2019: 4 · 2018: 2 · 2017: 5 · 2016: 4 · 2015: 2 · 2014: 2 · 2013: 5 · 2012: 4
FWCI
2.11
Type
journal article
Language
en
Open access
closed
Licence
www.springer.com/tdm (tdm)
Locations
  • Planta (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Fundação de Amparo à Pesquisa do Estado de São Paulo
  • Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
Topics
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Postharvest Quality and Shelf Life Management
  • Health Sciences › Nursing › Nutrition and Dietetics › Food composition and properties
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Banana Cultivation and Research
Keywords
starch phosphorylase, banana fruit, fruit development, fruit ripening, enzyme kinetics, branched glucan, protein synthesis regulation, gibberellic acid, indole-3-acetic acid, allosteric regulation, Musa acuminata
MeSH terms
Fruit, Gibberellins, Glucans, Glucosephosphates, Indoleacetic Acids, Isoenzymes, Kinetics, Molecular Weight, Phosphates, Plant Growth Regulators, Starch, Gene Expression Regulation, Enzymologic, Gene Expression Regulation, Plant, Gene Expression Regulation, Developmental, Starch Phosphorylase, Musa

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