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Cited by 58Photosynthetic Processes and MechanismsAlgal biology and biofuel productionEnzyme function and inhibition

Light-dependent bicarbonate uptake and CO 2 efflux in the marine microalga Nannochloropsis gaditana

Huertas, I. Emma, Espie, George S., Colman, Brian, Lubian, Luis M.·2000·Planta
DOI 10.1007/s004250000254
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Abstract

Inorganic carbon (Ci) uptake and efflux has been investigated in the marine microalga Nannochloropsis gaditana Lubian by monitoring CO2 fluxes in cell suspensions using mass spectrometry. Addition of H13CO3- to cell suspensions in the dark caused a transient increase in the CO2 concentration in the medium far in excess of the equilibrium CO2 concentration. The magnitude of this release was dependent on the length of time the cells had been kept in the dark. Once equilibrium between the Ci species had been achieved, a CO2 efflux was observed after saturating light intensity was applied to the cells. External carbonic anhydrase (CA) was not detected nor does this species demonstrate a capacity to take up CO2 by active transport. Photosynthetic O2 evolution and the release CO2 in the dark depend on HCO3- uptake since both were inhibited by the anion exchange inhibitor, 4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid (DIDS). The bicarbonate uptake mechanism requires light but can also continue for short periods in the dark. Ethoxyzolamide, a CA inhibitor, markedly inhibited CO2 efflux in the dark, indicating that CO2 efflux was dependent upon the intracellular dehydration of HCO3-. These results indicate that Nannochloropsis possesses a bicarbonate uptake system which causes the accumulation of high intracellular Ci levels and an internal CA which maintains the equilibrium between CO2 and HCO3- and thus causes a subsequent release of CO2 to the external medium.

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  • The finding that CO2 uptake continued in the dark during nitrogen depletion challenges the concept that HCO3− transport is strictly energized by the light reactions of photosynthesis.

    “This finding challenges the concept that HCO 3 2 transport is strictly energized by the light reactions of photosynthesis (Huertas et al., 2000).”

    From Responses of<i>Nannochloropsis oceanica</i>IMET1 to Long-Term Nitrogen Starvation and Recovery (2013) · cites this paper as Huertas et al., 2000

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Authors
Huertas, I. E., Espie, G. S., Colman, B., & Lubian, L. M.
Affiliations
  • Consejo Superior de Investigaciones Científicas (ES)
  • University of Toronto (CA)
  • York University (CA)
ORCID
Venue
Planta
Publisher
Springer Science and Business Media LLC
Published
2000-06-16
Volume
211
Issue
1
Pages
43-49
ISSN
  • 0032-0935 (ISSN-L)
  • 1432-2048
DOI
10.1007/s004250000254
OpenAlex
W59798374
Cited by
58
References
27
Citations by year
2026: 1 · 2024: 3 · 2023: 1 · 2022: 1 · 2021: 2 · 2020: 3 · 2019: 1 · 2018: 2 · 2017: 9 · 2016: 1 · 2015: 4 · 2014: 4 · 2013: 3 · 2012: 2
FWCI
0.96
Type
journal article
Language
en
Open access
closed
Licence
www.springer.com/tdm (tdm)
Locations
  • Planta (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Photosynthetic Processes and Mechanisms
  • Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Enzyme function and inhibition
Keywords
Nannochloropsis gaditana, bicarbonate uptake, CO2 efflux, carbonic anhydrase, DIDS inhibition, ethoxyzolamide, marine microalgae
MeSH terms
Bicarbonates, Carbon Dioxide, Darkness, Light, Eukaryota

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