Phylogenetic relationships of Habronema microstoma and Habronema muscae (Spirurida: Habronematidae) within the order Spirurida inferred using mitochondrial cytochrome c oxidase subunit 1 (cox1) gene analysis
Abstract
The present study investigated genetic variability within a population of Habronema microstoma and Habronema muscae (Spirurida: Habronematidae) affecting horses in an endemic area of central Italy using polymerase chain reaction (PCR)-coupled sequencing of the mitochondrial cytochrome c oxidase subunit 1 gene (cox1). No different cox1 sequences were detected in any of the H. muscae individual, while two haplotypes representing H. microstoma individuals differed for one substitution. The pairwise distance between the H. muscae and H. microstoma was 11%, coding for five amino acid changes. The sequence of an informative region within the cox1 gene of H. microstoma and H. muscae was analyzed by Maximum Likelihood and Bayesian phylogenetic methods using available mitochondrial sequences spirurid taxa belonging to Filarioidea, Thelazioidea, and Habronematoidea. Phylogenetic analysis supported the split of the tree into two sister spirurid groups, Habronematoidea and Filarioidea + Thelazioidea. The phylogenetic and evolutionary implications of Habronema with Filaroidea and Thelazioidea are discussed.
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PCR amplification targeting the mitochondrial COXI gene demonstrated high specificity and sensitivity for definitive species identification.
“By contrast, PCR amplification targeting the mitochondrial cytochrome c oxidase subunit I gene demonstrated high specificity and sensitivity for definitive species identification, consistent with the findings of previous molecular studies [6, 16].”
From Innovative diagnostic strategies for equine habronemiasis: exploring molecular identification, gene expression, and oxidative stress markers (2025) · cites this paper as [16]
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“PCR amplification targeted the COXI gene (689 base pairs) using specific degenerate primer pairs (forward (F): 5 ′ -TGA TTG GTG GTT TTG GTA A-3 ′ and reverse (R): 5 ′ -ATA AGT ACG AGT ATC AAT ATC-3 ′ ) developed by Iorio et al. [16].”
- Authors
- Iorio, R., Šlapeta, J., Otranto, D., Paoletti, B., Giangaspero, A., & Traversa, D.
- Affiliations
- University of Teramo (IT)
- The University of Sydney (AU)
- University of Bari Aldo Moro (IT)
- University of Foggia (IT)
- Corresponding author
- Traversa, Donato
- Venue
- Parasitology Research
- Published
- 2008-12-05
- Volume
- 104
- Issue
- 5
- Pages
- 979-984
- ISSN
- 0044-3255 (ISSN-L)
- 0932-0113
- 1432-1955
- OpenAlex
- W2012314345
- Cited by
- 13
- References
- 26
- Citations by year
- 2025: 1 · 2022: 1 · 2017: 2 · 2015: 2 · 2014: 2 · 2013: 2
- FWCI
- 1.54
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Parasitology Research (publishedVersion)
- PubMed (publishedVersion)
- UniTERAMO Research Catalog (University of Teramo) (submittedVersion)
- CINECA IRIS Institutional Research Information System (University of Bari Aldo Moro) (submittedVersion)
- Topics
- Health Sciences › Veterinary › Small Animals › Helminth infection and control
- Physical Sciences › Environmental Science › Ecology › Parasite Biology and Host Interactions
- Health Sciences › Medicine › Infectious Diseases › Parasitic Diseases Research and Treatment
- Keywords
- Habronema muscae, Habronematidae, Spirurida, mitochondrial cytochrome c oxidase subunit 1, cox1 haplotypes, phylogenetic relationships, horse parasites, Filarioidea, genetic variation, central Italy
- MeSH terms
- Animals, Electron Transport Complex IV, DNA, Mitochondrial, Haplotypes, Horse Diseases, Horses, Italy, Mitochondria, Molecular Sequence Data, Phylogeny, Polymorphism, Genetic, Spiruroidea, Spirurida Infections, Sequence Homology, Sequence Analysis, DNA, Amino Acid Substitution
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