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Cited by 137Insect Pest Control StrategiesEssential Oils and Antimicrobial ActivityDengue and Mosquito Control Research

The DOI matches its Crossref record; 45 of the 45 references it cites were found in a registry or an index.

α-Humulene and β-elemene from Syzygium zeylanicum (Myrtaceae) essential oil: highly effective and eco-friendly larvicides against Anopheles subpictus, Aedes albopictus, and Culex tritaeniorhynchus (Diptera: Culicidae)

Govindarajan, Marimuthu, Benelli, Giovanni·2016·Parasitology Research
DOI 10.1007/s00436-016-5025-2
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Abstract

Mosquitoes transmit serious pathogens and parasites to humans and animals, including malaria, dengue, Japanese encephalitis and filariasis. The extensive use of chemical pesticides leads to the development of resistance in mosquito vector populations and serious non-target effects on human health and the environment. Myrtaceae plants can be a useful reservoir of natural products effective against Culicidae young instars. In this research, we evaluated the mosquitocidal potential of the essential oil (EO) from Syzygium zeylanicum leaves against larvae of three mosquitoes of medical and veterinary importance, the malaria vector Anopheles subpictus, the dengue vector Aedes albopictus, and the Japanese encephalitis vector Culex tritaeniorhynchus. The chemical composition of the EO was analyzed by gas chromatography-mass spectroscopy. GC-MS revealed that the S. zeylanicum EO contained at least 18 compounds. The major chemical components were α-humulene (37.8.5 %) and β-elemene (10.7 %). The EO had a significant toxic effect against early third-stage larvae of An. subpictus, Ae. albopictus, and Cx. tritaeniorhynchus, with LC50 values of 83.11, 90.45, and 97.96 μg/ml, respectively. The two major constituents extracted from the S. zeylanicum EO were tested individually for acute toxicity against larvae of the three mosquito vectors. α-Humulene and β-elemene appeared highly effective against An. subpictus (LC50 = 6.19 and 10.26 μg/ml, respectively), followed by Ae. albopictus (LC50 = 6.86 and 11.15 μg/ml) and Cx. tritaeniorhynchus (LC50 = 7.39 and 12.05 μg/ml). Furthermore, the EO and its major components was safe towards the non-target fish Gambusia affinis; LC50 values were 20374.26, 1024.95, and 2073.18 μg/ml, respectively for EO, α-humulene and β-elemene. Overall, this study highlighted that the acute toxicity of S. zeylanicum EO towards mosquito larvae was mainly due to the presence of α-humulene and β-elemene. Furthermore, we pointed out the concrete possibility to exploit these two compounds from S. zeylanicum EO as highly effective larvicides against young instars of An. subpictus, Ae. albopictus, and Cx. tritaeniorhynchus, with little effect on non-target organisms.

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Crossref · 10.1007/s00436-016-5025-2

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Research works printed but not linked: 5

  1. Costa JGM, Rodrigues FFG, Angélico EC, Silva I, Mota ML, Santos NKA, Cardoso ALH, Lemos TLG (2005) Chemical–biological study of the essential oils of Hyptis martiusii, Lippia sidoides and Syzigium aromaticum against larvae of Aedes aegypti and Culex quinquefasciatus. Braz J Pharmacogenomics 15:304–309
  2. Finney DJ (1971) Probit analysis. Cambridge University Press, London, pp 68–72
  3. Mittal PK, Subbarao SK (2003) Prospects of using herbal products in mosquito control. ICMR Bull 33:1–10
  4. Pullaiah T (2006) Encyclopedia of world medicinal plants. Regency, Pune, pp 1895–1898
  5. WHO (2005) Guidelines for laboratory and field testing of mosquito larvicides. Communicable disease control, prevention and eradication, WHO pesticide evaluation scheme. WHO, Geneva, WHO/CDS/WHOPES/GCDPP/1.3

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Metadata

Authors
Govindarajan, M., & Benelli, G.
Affiliations
  • Annamalai University (IN)
  • University of Pisa (IT)
Corresponding author
  • Govindarajan, Marimuthu
  • Benelli, Giovanni
ORCID
Venue
Parasitology Research
Publisher
Springer Science and Business Media LLC
Published
2016-03-30
Volume
115
Issue
7
Pages
2771-2778
ISSN
  • 0044-3255 (ISSN-L)
  • 0932-0113
  • 1432-1955
DOI
10.1007/s00436-016-5025-2
OpenAlex
W2471243309
Cited by
137
References
45
Citations by year
2026: 5 · 2025: 6 · 2024: 13 · 2023: 19 · 2022: 16 · 2021: 14 · 2020: 14 · 2019: 8 · 2018: 9 · 2017: 20 · 2016: 13
FWCI
19.52
Type
journal article
Language
en
Open access
closed
Licence
www.springer.com/tdm (tdm)
Locations
  • Parasitology Research (publishedVersion)
  • PubMed (publishedVersion)
  • CINECA IRIS Institutial research information system (University of Pisa) (submittedVersion)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Insect Pest Control Strategies
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Essential Oils and Antimicrobial Activity
  • Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Dengue and Mosquito Control Research
Keywords
Syzygium zeylanicum, essential oils, α-humulene, β-elemene, larvicidal activity, mosquito vectors, Anopheles subpictus, Aedes albopictus, Culex tritaeniorhynchus, malaria vectors, dengue vector, Japanese encephalitis vector, natural insecticides, Gambusia affinis, non-target toxicity, larval development inhibition, Myrtaceae, chemical composition, LC50, mosquito-borne diseases, pesticide resistance
MeSH terms
Mosquito Vectors, Monocyclic Sesquiterpenes, Aedes, Animals, Anopheles, Culex, Insecticides, Larva, Gas Chromatography-Mass Spectrometry, Oils, Volatile, Sesquiterpenes, Plant Leaves, Myrtaceae, Syzygium

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