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Cited by 125Fungal and yeast genetics researchSignaling Pathways in DiseaseBacterial Genetics and Biotechnology

The DOI matches its Crossref record; 42 of the 42 references it cites were found in a registry or an index.

Impairment of calcineurin function in Neurospora crassa reveals its essential role in hyphal growth, morphology and maintenance of the apical Ca2+ gradient

Prokisch, H., Yarden, O., Dieminger, M., Tropschug, M., Barthelmess, I. B.·1997·Molecular and General Genetics MGG
DOI 10.1007/s004380050551
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Abstract

The function of Neurospora crassa calcineurin was investigated in N. crassa strains transformed with a construct that provides for the inducible expression of antisense RNA for the catalytic subunit of calcineurin (cna-1). Induction of antisense RNA expression was associated with reduced levels of cna-1 mRNA and of immunodetectable CNA1 protein and decreased calcineurin enzyme activity, indicating that a conditional reduction of the target function had been achieved in antisense transformants with multiple construct integrations. Induction conditions caused growth arrest which indicated that the cna-1 gene is essential for growth of N. crassa. Growth arrest was preceded by an increase in hyphal branching, changes in hyphal morphology and concomitant loss of the distinctive tip-high Ca2+ gradient typical for growing wild-type hyphae. This demonstrates a novel and specific role for calcineurin in the precise regulation of apical growth, a common form of cellular proliferation. In vitro inhibition of N. crassa calcineurin by the complex of cyclosporin A (CsA) and cyclophilin20, and increased sensitivity of the induced transformants to the calcineurin-specific drugs CsA and FK506 imply that the drugs act in N. crassa, as in T-cells and Saccharomyces cerevisiae, by inactivating calcineurin. The finding that exposure of growing wild-type mycelium to these drugs leads to a phenotype very similar to that of the cna-1 antisense mutants is consistent with this idea.

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Metadata

Authors
Prokisch, H., Yarden, O., Dieminger, M., Tropschug, M., & Barthelmess, I. B.
Affiliations
  • Leibniz University Hannover (DE)
  • Hebrew University of Jerusalem (IL)
  • University of Freiburg (DE)
ORCID
Venue
Molecular and General Genetics MGG
Publisher
Springer Science and Business Media LLC
Published
1997-09
Volume
256
Issue
2
Pages
104-114
ISSN
  • 0026-8925 (ISSN-L)
  • 1432-1874
DOI
10.1007/s004380050551
OpenAlex
W2049238469
Cited by
125
References
42
Citations by year
2023: 2 · 2022: 1 · 2020: 3 · 2019: 3 · 2017: 3 · 2016: 3 · 2015: 4 · 2014: 9 · 2013: 4 · 2012: 5
FWCI
2.73
Type
journal article
Language
en
Open access
closed
Licence
www.springer.com/tdm (tdm)
Locations
  • Molecular and General Genetics MGG (publishedVersion)
  • PubMed (publishedVersion)
  • FreiDok plus (Universitätsbibliothek Freiburg) (submittedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Signaling Pathways in Disease
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
Keywords
calcineurin, Neurospora crassa, antisense RNA, hyphal growth, hyphal branching, hyphal morphology, cyclosporine, cyclophilin, FK506, calcineurin inhibitors, essential genes, mycelium
MeSH terms
Calcium, Cloning, Molecular, Immunosuppressive Agents, Neurospora crassa, Plasmids, Recombination, Genetic, RNA, Messenger, Transcription, Genetic, Transformation, Genetic, Gene Expression Regulation, Fungal, Gene Expression Regulation, Enzymologic, RNA, Antisense, Tacrolimus, Cyclosporine, Peptidylprolyl Isomerase, Calcineurin

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