Cited by 28Planarian Biology and ElectrostimulationMarine Invertebrate Physiology and EcologyNeuropeptides and Animal Physiology

Effects of neuropeptide F on regeneration in Girardia tigrina (Platyhelminthes)

Kreshchenko, Natalia D., Sedelnikov, Zakhar, Sheiman, Inna M., Reuter, Maria, Maule, Aaron G., Gustafsson, Margaretha K. S.·2008·Cell and Tissue Research

Abstract

The effects of neuropeptide F (NPF; from Moniezia expansa) on the regeneration of Girardia tigrina were studied. The animals were decapitated and incubated in water (control) or NPF. The dynamics of the proliferation of the neoblasts in the developing tissue were studied during the course of regeneration by monitoring the mitotic index (MI). The effects of incubation in FMRFamide and GYIRFamide on the MI were also tested. The course of cephalic regeneration was followed with in vivo computer-assisted morphometry for up to 7 days. The development of the regenerating nervous system and the musculature was visualised by immunostaining with a primary antiserum to the C-terminal decapeptide of NPF (YFAIIGRPRFa) and tetramethylrhodamine-isothiocyanate-conjugated phalloidin, which stains F-actin in muscle filaments. The study showed that NPF had a stimulatory effect on the mitotic activity of the neoblasts. FMRFamide and GYIRFamide did not have this effect. NPF also stimulated the growth of the regenerating head and the growing nervous system and musculature. NPF is postulated to have a morphogenetic action in the regenerating animals.

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Crossref · 10.1007/s00441-007-0519-y

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64 of 64 references verified0 citation errors · 0 not checkable

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  • The nervous system affects growth and regeneration.

    “Growth and regeneration are also influenced by the nervous system [84,85].”

    From An option space for early neural evolution (2015) · cites this paper as [84]

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Authors
Kreshchenko, N. D., Sedelnikov, Z., Sheiman, I. M., Reuter, M., Maule, A. G., & Gustafsson, M. K. S.
Affiliations
  • Institute of Cell Biophysics (RU)
  • Åbo Akademi University (FI)
  • Queen's University Belfast (GB)
Corresponding author
Gustafsson, Margaretha K. S.
Venue
Cell and Tissue Research
Published
2007-12-20
Volume
331
Issue
3
Pages
739-750
ISSN
  • 0302-766X (ISSN-L)
  • 1432-0878
OpenAlex
W2042501003
Cited by
28
References
64
Citations by year
2024: 1 · 2022: 2 · 2020: 4 · 2018: 3 · 2017: 2 · 2016: 3 · 2015: 2 · 2013: 1 · 2012: 4
FWCI
0.39
Type
journal article
Language
en
Open access
closed
Locations
  • Cell and Tissue Research (publishedVersion)
  • PubMed (publishedVersion)
  • Research Portal (Queen's University Belfast) (submittedVersion)
Funders
Wellcome Trust (069411/Z/02/Z)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Planarian Biology and Electrostimulation
  • Physical Sciences › Earth and Planetary Sciences › Paleontology › Marine Invertebrate Physiology and Ecology
  • Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuropeptides and Animal Physiology
Keywords
neuropeptide F, Girardia tigrina, regeneration, mitotic index, FMRFamide, nervous system regeneration, muscle regeneration, Moniezia expansa
MeSH terms
Actins, Amino Acids, Animals, Brain, Brain Chemistry, Colchicine, Head, Mitosis, Mitotic Index, Muscles, Nerve Regeneration, Neuropeptides, Oligopeptides, Phalloidine, Regeneration, Turbellaria, FMRFamide, Decapitation

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