Shear conditions in clavulanic acid production by Streptomyces clavuligerus in stirred tank and airlift bioreactors
Abstract
In biochemical processes involving filamentous microorganisms, the high shear rate may damage suspended cells leading to viability loss and cell disruption. In this work, the influence of the shear conditions in clavulanic acid (CA) production by Streptomyces clavuligerus was evaluated in a 4-dm(3) conventional stirred tank (STB) and in 6-dm(3) concentric-tube airlift (ALB) bioreactors. Batch cultivations were performed in a STB at 600 and 800 rpm and 0.5 vvm (cultivations B1 and B2) and in ALB at 3.0 and 4.1 vvm (cultivations A1 and A2) to define two initial oxygen transfer conditions in both bioreactors. The average shear rate ([Formula: see text]) of the cultivations was estimated using correlations of recent literature based on experimental data of rheological properties of the broth (consistency index, K, and flow index, n) and operating conditions, impeller speed (N) for STB and superficial gas velocity in the riser (UGR) for ALB. In the same oxygen transfer condition, the [Formula: see text] values for ALB were higher than those obtained in STB. The maximum [Formula: see text] presented a strong correlation with a maximum consistency index (K (max)) of the broth. Close values of maximum CA production were obtained in cultivations A1 and A2 (454 and 442 mg L(-1)) with similar maximum [Formula: see text] values of 4,247 and 4,225 s(-1). In cultivations B1 and B2, the maximum CA production of 269 and 402 mg L(-1) were reached with a maximum [Formula: see text] of 904 and…
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A pH of 6.8 is favorable for obtaining high titers of CA in submerged cultures.
“Previous studies have shown that CA stability increases at slightly acidic pH values [10,11]; several authors found a pH of 6.8 as favorable for obtaining high titers of CA in submerged cultures [5,7,9,12,13,14,15,16].”
From Degradation Kinetics of Clavulanic Acid in Fermentation Broths at Low Temperatures (2019) · cites this paper as [15]
Cerri and Badino (2012) supported the view that an increase in agitation speed leads to higher clavulanic acid production, but not oxygen flow.
“A study by Cerri and Badino (2012) also supported the view that an increase in agitation speed leads to higher production of clavulanic acid, but not oxygen flow.”
From Fermentation Conditions that Affect Clavulanic Acid Production in Streptomyces clavuligerus: A Systematic Review (2016) · cites this paper as Cerri and Badino (2012)
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Ser, H.-L., Law, J. W.-F., Chaiyakunapruk, N., Jacob, S. A., Palanisamy, U. D., Chan, K.-G., Goh, B.-H., & Lee, L.-H. (2016) · Frontiers in Microbiology · doi:10.3389/fmicb.2016.00522 · Cited by 90 · see citing works
“A study by Cerri and Badino (2012) also supported the view that an increase in agitation speed leads to higher production of clavulanic acid, but not oxygen flow.”
BackgroundSparger design as key parameter to define shear conditions in pneumatic bioreactors
Esperança, M. N., Mendes, C. E., Rodriguez, G. Y., Cerri, M. O., Béttega, R., & Badino, A. C. (2020) · Biochemical Engineering Journal · doi:10.1016/j.bej.2020.107529 · Cited by 20 · see citing works
Self-citationDegradation Kinetics of Clavulanic Acid in Fermentation Broths at Low Temperatures
Gómez-Ríos, D., Ramírez-Malule, H., Neubauer, P., Junne, S., & Ríos-Estepa, R. (2019) · Antibiotics · doi:10.3390/antibiotics8010006 · Cited by 17 · see citing works
- Authors
- Cerri, M. O., & Badino, A. C.
- Affiliations
- Federal University of São João del-Rei (BR)
- ORCID
- Cerri, M. O.
- Venue
- Bioprocess and Biosystems Engineering
- Published
- 2012-01-24
- Volume
- 35
- Issue
- 6
- Pages
- 977-984
- ISSN
- 1615-7591 (ISSN-L)
- 1615-7605
- OpenAlex
- W1997911734
- Cited by
- 28
- References
- 0
- Citations by year
- 2026: 1 · 2025: 2 · 2024: 3 · 2023: 1 · 2022: 1 · 2021: 2 · 2020: 3 · 2019: 3 · 2018: 2 · 2016: 4 · 2015: 1 · 2014: 3 · 2013: 2
- FWCI
- 1.09
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Bioprocess and Biosystems Engineering (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Fundação de Amparo à Pesquisa do Estado de São Paulo
- Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
- Conselho Nacional de Desenvolvimento Científico e Tecnológico
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › Fluid Dynamics and Mixing
- Health Sciences › Medicine › Pharmacology › Microbial Natural Products and Biosynthesis
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Keywords
- clavulanic acid production, Streptomyces clavuligerus, shear rate, airlift bioreactor, stirred tank bioreactor, filamentous microorganisms, cell damage, cell viability, consistency index, oxygen transfer, impeller speed, superficial gas velocity
- MeSH terms
- Models, Biological, Oxygen, Oxygen Consumption, Streptomyces, Stress, Physiological, Bioreactors, Clavulanic Acid
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