Statistical approach to evaluate effect of temperature and moisture content on the production of antioxidant naphtho-gamma-pyrones and hydroxycinnamic acids by Aspergillus tubingensis in solid-state fermentation
Open accessAbstract
Non-ochratoxigenic Aspergillus tubingensis G131 is a filamentous fungus that can produce naphtho-gamma-pyrones (NγPs), polyketide pigments that exhibit interesting antioxidant properties. This study aims to investigate the effect of two critical parameters, temperature and moisture content on the fungus grown in solid-state fermentation using agricultural by-products (vine shoots and wheat bran) as sole medium. From the kinetic productions of secondary metabolites NγPs (asperpyrone E, dianhydroaurasperone C, fonsecin, fonsecin B and ustilaginoidin A), alkaloids (nigragilin and aspernigrin A), degradation products from the solid medium (β-D-glucose, p-coumaric acid and trans-ethyl ferulate), ergosterol and conidia obtained for different temperatures and moisture contents, a principal component analysis (PCA) was carried out to highlight the production patterns of these compounds. This approach allowed us to determine that fonsecin, the compound of higher interest-exhibiting the most interesting antiradical potential-is particularly more produced at 25 °C and 66% of moisture content. This study underlines the importance of temperature and moisture content on naphtho-gamma-pyrones and hydroxycinnamic acid production using solid-state fermentation and contributes to the development of agroindustrial by-product valorization.
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Temperature can affect the fluidity of the plasma membrane, the activity of enzymes, and the production of secondary metabolites during fermentation.
“In addition, temperature can affect the fluidity of the plasma membrane, the activity of enzymes, and the production of secondary metabolites during fermentation [29,32].”
From Optimization of Fermentation Conditions for 2,3,5-Trimethylpyrazine Produced by Bacillus amyloliquefaciens from Daqu (2024) · cites this paper as [29]
The Results and discussion section reports that temperature changes secondary-metabolite production and suggests a temperature optimum for fungal esterases; it also reports a temperature optimum for enzyme activity in another strain. Neither it nor the Introduction addresses plasma-membrane fluidity, so that part of the claim is unsupported. (LLM, conf=0.86)
Experimental — accuracy not yet measured
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Cites
Principal component analysis: A review and recent developments
Jolliffe, I. T., & Cadima, J. (2016) · Philosophical Transactions of the Royal Society A: Mathematical, Physical and Engineering Sciences · doi:10.1098/rsta.2015.0202 · Cited by 10,117 · see citing works
THE USE OF THE STABLE FREE RADICAL DIPHENYLPICRYLHYDRAZYL (DPPH) FOR ESTIMATING ANTIOXIDANT ACTIVITY
Molyneux, P. (2004) · Songklanakarin Journal of Science and Technology · Cited by 2,771 · see citing works
Fungal secondary metabolism: regulation, function and drug discovery
Keller, N. P. (2018) · Nature Reviews Microbiology · doi:10.1038/s41579-018-0121-1 · Cited by 1,410 · see citing works
Pandey, A. (2003) · Biochemical Engineering Journal · doi:10.1016/s1369-703x(02)00121-3 · Cited by 1,243 · see citing works
(2019) · Advances in biochemical engineering, biotechnology · doi:10.1007/978-3-030-23675-5 · Cited by 1,106 · see citing works
Recent advances in solid-state fermentation
Singhania, R. R., Patel, A. K., Soccol, C. R., & Pandey, A. (2008) · Biochemical Engineering Journal · doi:10.1016/j.bej.2008.10.019 · Cited by 922 · see citing works
Recent developments and innovations in solid state fermentation
Soccol, C. R., da Costa, E. S. F., Letti, L. A. J., Karp, S. G., Woiciechowski, A. L., & Vandenberghe, L. P. D. S. (2017) · Biotechnology Research and Innovation · doi:10.1016/j.biori.2017.01.002 · Cited by 525 · see citing works
Ergosterol-to-Biomass Conversion Factors for Aquatic Hyphomycetes
Gessner, M. O., & Chauvet, É. (1993) · Applied and Environmental Microbiology · doi:10.1128/aem.59.2.502-507.1993 · Cited by 470 · see citing works
Production of cellulases and hemicellulases by Aspergillus niger KK2 from lignocellulosic biomass
Kang, S.-W. (2003) · Bioresource Technology · doi:10.1016/s0960-8524(03)00172-x · Cited by 432 · see citing works
Frisvad, J. C., Møller, L. L. H., Larsen, T. O., Kumar, R. R., & Arnau, J. (2018) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-018-9354-1 · Cited by 387 · see citing works
Biodiversity of Aspergillus species in some important agricultural products
Perrone, G., Susca, A., Cozzi, G., Ehrlich, K., Varga, J., Frisvad, J. C., Meijer, M., Noonim, P., Mahakarnchanakul, W., & Samson, R. A. (2007) · Studies in Mycology · doi:10.3114/sim.2007.59.07 · Cited by 369 · see citing works
Diagnostic tools to identify black aspergilli
Samson, R. A., Noonim, P., Meijer, M., et al. (2007) · Studies in Mycology · doi:10.3114/sim.2007.59.13 · Cited by 353 · see citing works
Ergosterol and microbial biomass relationship in soil
Djajakirana, G., Joergensen, R. G., & Meyer, B. (1996) · Biology and Fertility of Soils · doi:10.1007/bf00334573 · Cited by 350 · see citing works
Nielsen, K. F., Holm, G., Uttrup, L. P., & Nielsen, P. (2004) · International Biodeterioration & Biodegradation · doi:10.1016/j.ibiod.2004.05.002 · Cited by 314 · see citing works
Potential applications of antioxidants – A review
Sindhi, V., Gupta, V., Sharma, K., et al. (2013) · Journal of Pharmacy Research · doi:10.1016/j.jopr.2013.10.001 · Cited by 310 · see citing works
Solid-State Fermentation as a Novel Paradigm for Organic Waste Valorization: A Review
Yazid, N. A., Barrena, R., Komilis, D. P., & Sánchez, A. (2017) · Sustainability · doi:10.3390/su9020224 · Cited by 282 · see citing works
Dilokpimol, A., Mäkelä, M., Pontes, M. V. A., Benoit, I., Hildén, K., & de Vries, R. P. (2016) · Biotechnology for Biofuels · doi:10.1186/s13068-016-0651-6 · Cited by 173 · see citing works
Bioactive natural products derived from mangrove-associated microbes
Xu, J. (2014) · RSC Advances · doi:10.1039/c4ra11756e · Cited by 136 · see citing works
Citric and gluconic acid production from fig by Aspergillus niger using solid-state fermentation
Roukas, T. (2000) · Journal of Industrial Microbiology & Biotechnology · doi:10.1038/sj.jim.7000101 · Cited by 105 · see citing works
Pardo, E., Marı́n, S., Sanchís, V., et al. (2004) · International Journal of Food Microbiology · doi:10.1016/j.ijfoodmicro.2004.02.003 · Cited by 102 · see citing works
Parra‐Saldívar, R., & Magan, N. (2004) · Journal of Applied Microbiology · doi:10.1111/j.1365-2672.2004.02320.x · Cited by 86 · see citing works
Relationship of mould count, ergosterol and ochratoxin A production
Saxena, J., Munimbazi, C., & Bullerman, L. B. (2001) · International Journal of Food Microbiology · doi:10.1016/s0168-1605(01)00584-0 · Cited by 78 · see citing works
Improvement of heat removal in solid‐state fermentation tray bioreactors by forced air convection
Figueroa‐Montero, A., Esparza‐Isunza, T., Saucedo‐Castañeda, G., et al. (2011) · Journal of Chemical Technology & Biotechnology · doi:10.1002/jctb.2637 · Cited by 77 · see citing works
Hamrouni, R., Molinet, J., Mitropoulou, G., Yiannis, K., Nathalie, D., Ahmed, M., & Roussos, S. (2019) · Journal of Environmental Management · doi:10.1016/j.jenvman.2019.109496 · Cited by 75 · see citing works
Self-citationTrivedi, S., Divecha, J., & Shah, A. R. (2012) · Carbohydrate Polymers · doi:10.1016/j.carbpol.2012.05.068 · Cited by 68 · see citing works
García‐Cela, E., Crespo-Sempere, A., Ramos, A. J., et al. (2013) · International Journal of Food Microbiology · doi:10.1016/j.ijfoodmicro.2013.12.012 · Cited by 50 · see citing works
Max, B., Torrado, A., Moldes, A. B., et al. (2008) · Biochemical Engineering Journal · doi:10.1016/j.bej.2008.09.015 · Cited by 50 · see citing works
Salgado, J. M., Abrunhosa, L., Venâncio, A., et al. (2015) · Journal of Agricultural and Food Chemistry · doi:10.1021/acs.jafc.5b02131 · Cited by 47 · see citing works
Fungal naphtho-γ-pyrones—secondary metabolites of industrial interest
Choque, É., Rayess, Y. E., Raynal, J., & Mathieu, F. (2014) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-014-6295-1 · Cited by 46 · see citing works
Lahouar, A., Marı́n, S., Crespo-Sempere, A., et al. (2016) · International Journal of Food Microbiology · doi:10.1016/j.ijfoodmicro.2016.11.015 · Cited by 43 · see citing works
Choque, É., Klopp, C., Valière, S., Raynal, J., & Mathieu, F. (2018) · BMC Genomics · doi:10.1186/s12864-018-4574-4 · Cited by 37 · see citing works
Carboué, Q., Claeys‐Bruno, M., Bombarda, I., Sergent, M., Jolain, J., & Roussos, S. (2018) · Chemometrics and Intelligent Laboratory Systems · doi:10.1016/j.chemolab.2018.03.011 · Cited by 34 · see citing works
Self-citationSteudler, S., & Bley, T. (2015) · Bioprocess and Biosystems Engineering · doi:10.1007/s00449-015-1372-0 · Cited by 33 · see citing works
Hegde, S., & Muralikrishna, G. (2009) · World Journal of Microbiology and Biotechnology · doi:10.1007/s11274-009-0095-2 · Cited by 33 · see citing works
Pérez-Rodríguez, N., Moreira, C. D., Torrado, A., et al. (2016) · Enzyme and Microbial Technology · doi:10.1016/j.enzmictec.2016.05.011 · Cited by 22 · see citing works
Bouras, N., Mathieu, F., Coppel, Y., et al. (2007) · Journal of Agricultural and Food Chemistry · doi:10.1021/jf071406z · Cited by 20 · see citing works
Carboué, Q., Rébufa, C., Dupuy, N., Roussos, S., & Bombarda, I. (2019) · Biochemical Engineering Journal · doi:10.1016/j.bej.2019.107248 · Cited by 19 · see citing works
Self-citationCarboué, Q., Maresca, M., Herbette, G., et al. (2019) · Biomolecules · doi:10.3390/biom10010029 · Cited by 19 · see citing works
Self-citationModeling the microbial growth and temperature profile in a fixed-bed bioreactor
da Silveira, C. L., Mazutti, M. A., & Salau, N. P. G. (2014) · Bioprocess and Biosystems Engineering · doi:10.1007/s00449-014-1170-0 · Cited by 13 · see citing works
Influence of the culture conditions on the production of NGPs by Aspergillus tubingensis
de leon, L. L., Caceres, I., Bornot, J., et al. (2019) · Journal of Microbiology and Biotechnology · doi:10.4014/jmb.1905.05015 · Cited by 12 · see citing works
Vine Trimming Shoots as Substrate for Ferulic Acid Esterases Production
Pérez-Rodríguez, N., Outeiriño, D., Torrado, A., et al. (2016) · Applied Biochemistry and Biotechnology · doi:10.1007/s12010-016-2251-0 · Cited by 8 · see citing works
Imran, M., Hussain, A., Anwar, Z., et al. (2017) · Iranian Journal of Science and Technology Transactions A Science · doi:10.1007/s40995-017-0462-z · Cited by 6 · see citing works
Dhungana, B., Ali, S., Byamukama, E., et al. (2019) · Cereal Chemistry · doi:10.1002/cche.10199 · Cited by 4 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Kumar, V., Ahluwalia, V., Saran, S., Kumar, J., Patel, A. K., & Singhania, R. R. (2021) · Bioresource Technology · doi:10.1016/j.biortech.2020.124566 · Cited by 357 · see citing works
Liu, X., Yang, W., Gu, H., Bughio, A. A., & Liu, J. (2024) · Fermentation · doi:10.3390/fermentation10020112 · Cited by 16 · see citing works
- Authors
- Carboué, Q., Rébufa, C., Hamrouni, R., Roussos, S., & Bombarda, I.
- Affiliations
- Centre National de la Recherche Scientifique (FR)
- Corresponding author
- Carboué, Quentin
- Bombarda, Isabelle
- Venue
- Bioprocess and Biosystems Engineering
- Published
- 2020-09-03
- Volume
- 43
- Issue
- 12
- Pages
- 2283-2294
- ISSN
- 1615-7591 (ISSN-L)
- 1615-7605
- OpenAlex
- W3083747476
- Cited by
- 35
- References
- 43
- Citations by year
- 2026: 7 · 2025: 10 · 2024: 8 · 2023: 3 · 2022: 5 · 2021: 1 · 2020: 1
- FWCI
- 1.29
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Bioprocess and Biosystems Engineering (publishedVersion, other-oa)
- PubMed (publishedVersion)
- HAL (submittedVersion, other-oa)
- Funders
- Association Nationale de la Recherche et de la Technologie
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Microbial Metabolism and Applications
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Mycotoxins in Agriculture and Food
- Health Sciences › Medicine › Pharmacology › Microbial Natural Products and Biosynthesis
- Keywords
- Aspergillus tubingensis, hydroxycinnamic acids, solid-state fermentation, temperature effects, moisture content, fungal secondary metabolites, antioxidant pigments, agricultural by-product valorization, vine shoots, wheat bran, principal component analysis, free radical scavenging
- MeSH terms
- Antioxidants, Aspergillus, Chromatography, High Pressure Liquid, Coumaric Acids, Dihydropyridines, Ergosterol, Fermentation, Heterocyclic Compounds, 3-Ring, Kinetics, Naphthols, Plants, Pyrones, Temperature, Models, Statistical, Principal Component Analysis
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
