Assessment of genetic stability of two micropropagated wild olive species using flow cytometry and microsatellite markers
Open accessAbstract
Micropropagated plants from two wild-olive species, Olea maderensis and O. europaea ssp. europaea var. sylvestris were screened for genetic stability. O. maderensis shoots were elongated/multiplied on OMG medium with zeatin (9.12 μM), and rooted on 1/2 OMG with NAA (3.22 μM). O. europaea var. sylvestris shoots were elongated/multiplied on OM medium with zeatin, and rooting was optimal after a hormonal shock (IBA 100 μM) followed by transfer to the same medium without growth regulators. In both species, acclimatization was successful and plants looked normal and morphologically identical to the donor field trees. Genetic variability was assessed at several stages of the micropropagation process using flow cytometry (FCM) and nuclear microsatellites (SSR). No changes in ploidy level were found among micropropagated plants, though small deviations, putatively due to the negative effects of cytosolic compounds on propidium iodide staining, between these and field plants were observed. In SSRs analyses, ten SSR markers were able to distinguish between genotypes. No mutations were found in these tested SSR loci among the donor tree and micropropagated plants, suggesting, for the tested markers, genetic uniformity throughout the process. The FCM and SSR results obtained do not exclude the occurrence of other changes in the nuclear genome but, considering the morphological stability of micropropagated plants, indicate that both protocols are suitable and efficient for large scale, tr…
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Nuclear DNA content in olive species was significantly different between donor plants and acclimatized plants in highly reproducible histograms.
“Moreover, nuclear DNA content in olive species was significantly different between donor plants and acclimatized plants in highly reproducible histograms [20].”
Ploidy level stability assessment in olive cultivar sylvestris multiplied on OM with zeatin after a PGR shock showed DNA content reduced 2.5-9% in micropropagated plants.
“Ploidy level stability assessment in olive cultivar ˈsylvestrisˈ multiplied on OM with zeatin after a PGR shock followed by transferring to the same medium without PGRs showed the DNA content reduced 2.5–9% in the micropropagated plants [20].”
Stem cutting in vitro culture in olive was stable.
“Since stem cutting in vitro culture in olive was stable, therefore 97% resemblance between ˈAminˈ MIS and ˈArbequinaˈ MIS proved high similarity between these cultivars [20, 23, 33].”
The 97% resemblance between ˈAminˈ MIS and ˈArbequinaˈ MIS proved high similarity between these cultivars.
“Since stem cutting in vitro culture in olive was stable, therefore 97% resemblance between ˈAminˈ MIS and ˈArbequinaˈ MIS proved high similarity between these cultivars [20, 23, 33].”
Cited by
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Mirzaei, L., Yadollahi, A., Kermani, M. J., Naderpour, M., Zeinanloo, A. A., Farsi, M., & Davoodi, D. (2021) · Plant Methods · doi:10.1186/s13007-021-00724-7 · Cited by 16 · see citing works
Bychappa, M., Mishra, M. K., Jingade, P., & Huded, A. K. C. (2019) · Plant Cell, Tissue and Organ Culture (PCTOC) · doi:10.1007/s11240-019-01666-8 · Cited by 13 · see citing works
- Authors
- Brito, G., Lopes, T., Loureiro, J., Rodriguez, E., & Santos, C.
- Affiliations
- Centro de Estudos do Ambiente e do Mar (PT)
- Corresponding author
- Brito, Gina
- Venue
- Trees
- Published
- 2010-05-25
- Volume
- 24
- Issue
- 4
- Pages
- 723-732
- ISSN
- 0931-1890 (ISSN-L)
- 1432-2285
- OpenAlex
- W1989109447
- Cited by
- 44
- References
- 0
- Citations by year
- 2025: 2 · 2024: 2 · 2023: 3 · 2022: 5 · 2021: 3 · 2020: 1 · 2019: 4 · 2018: 4 · 2017: 5 · 2016: 2 · 2015: 3 · 2014: 3 · 2013: 3 · 2012: 1
- FWCI
- 0.91
- Type
- journal article
- Language
- en
- Open access
- green
- hdl.handle.net
- Licence
- Locations
- Trees (publishedVersion)
- Portuguese National Funding Agency for Science, Research and Technology (RCAAP Project by FCT) (submittedVersion, cc-by)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant tissue culture and regeneration
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetic diversity and population structure
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Chromosomal and Genetic Variations
- Keywords
- genetic stability, micropropagation, wild olive, Olea europaea var. sylvestris, ploidy level, microsatellite markers, flow cytometry, acclimatization, shoot multiplication, rooting, genotype discrimination
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