Effect of TNF-α inhibition on urinary albumin excretion in experimental diabetic rats
Abstract
The objective is to assess the effect of TNF-alpha inhibition on urinary albumin excretion in experimental diabetic rats. Male Wistar rats, 8-week-old, were categorized into four groups, which were the control (n = 9), diabetes (n = 9), infliximab-treated diabetes (n = 10), and FR167653-treated diabetes (n = 9) groups. Diabetes was induced by intraperitoneal injection of STZ (40 mg/kg). Thereafter, infliximab was injected intraperitoneally once a month (5.5 mg/kg) and FR167653 was administered orally by mixing with the rat chow (0.08%). The effects of infliximab and FR167653 on urinary albumin excretion were observed for 12 weeks. Body weight, blood sugar, 24-h urinary TNF-alpha, and 24-h urinary albumin/creatinine ratio (Ualb/Ucr) levels were determined at 1, 4, 8, and 12 weeks after the STZ-injection. Treatment of rats with STZ caused a significant loss of body weight, as well as polyuria and hyperglycemia within 1 week, while the urinary excretions of albumin and TNF-alpha were increased. Neither infliximab nor FR167653 affected body weight or blood sugar levels, whereas both decreased urinary albumin excretion, together with a modest decrease in the urinary excretion of TNF-alpha. These results suggest a role of TNF-alpha in the pathogenesis of diabetic nephropathy and show that TNF-alpha inhibition is a potential therapeutic strategy.
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Animal experiments have shown that TNF-α-blocking Infliximab improves renal function through attenuating fibrosis and decreasing albumin excretion in a diabetic nephropathy rat model.
“Furthermore, animal experiments have shown that TNF-α-blocking Infliximab improves renal function through attenuating fibrosis 20 and decreasing albumin excretion in a diabetic nephropathy rat model, 21,22”
From The effect of <scp>GLP</scp> ‐1 receptor agonists on circulating inflammatory markers in type 2 diabetes patients: A systematic review and meta‐analysis (2025) · cites this paper as [22]
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- Authors
- Moriwaki, Y., Inokuchi, T., Yamamoto, A., Ka, T., Tsutsumi, Z., Takahashi, S., & Yamamoto, T.
- Affiliations
- Hyogo Medical University (JP)
- Corresponding author
- Moriwaki, Yuji
- ORCID
- Yamamoto, Tetsuya
- Venue
- Acta Diabetologica
- Published
- 2007-09-01
- Volume
- 44
- Issue
- 4
- Pages
- 215-218
- ISSN
- 0940-5429 (ISSN-L)
- 1432-5233
- OpenAlex
- W2035793960
- Cited by
- 110
- References
- 19
- Citations by year
- 2025: 4 · 2024: 1 · 2023: 6 · 2022: 8 · 2021: 6 · 2020: 9 · 2019: 6 · 2018: 4 · 2017: 6 · 2016: 4 · 2015: 7 · 2014: 9 · 2013: 5 · 2012: 15
- FWCI
- 3.86
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Acta Diabetologica (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Nephrology › Chronic Kidney Disease and Diabetes
- Health Sciences › Medicine › Nephrology › Renal Diseases and Glomerulopathies
- Health Sciences › Medicine › Epidemiology › Adipokines, Inflammation, and Metabolic Diseases
- Keywords
- anti-TNF therapy, urinary albumin excretion, diabetic nephropathy, infliximab, FR167653, streptozotocin-induced diabetes, TNF-α, albuminuria
- MeSH terms
- Infliximab, Albuminuria, Animals, Anti-Inflammatory Agents, Non-Steroidal, Antibodies, Monoclonal, Diabetes Mellitus, Experimental, Male, Pyrazoles, Pyridines, Tumor Necrosis Factor-alpha, Rats, Wistar, Rats
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