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Detection of respiratory viruses using a multiplex real-time PCR assay in Germany, 2009/10
Open accessAbstract
The aim of this study was to determine the prevalence of respiratory viruses and to prospectively evaluate the performance of the fast-track diagnostics (FTD) respiratory pathogens multiplex PCR assay shortly after the 2009/10 influenza pandemic. Highly sensitive monoplex real-time PCR assays served as references. Discrepant results were further analyzed by the xTAG RVP Fast assay. A total of 369 respiratory samples from children and adults were collected prospectively in Germany from December 2009 until June 2010. The sensitivity and specificity of the FTD assay after resolution of discrepant results was 92.2 % and 99.5 %, respectively. Lowest specificity of the FTD assay was observed for human bocavirus. Multiple detections were recorded in 33/369 (8.9 %) of the samples by monoplex PCR and in 43/369 (11.7 %) using the FTD assay. The most prevalent viruses were respiratory syncytial virus and human metapneumovirus. Only pandemic influenza virus A/H1N1 (2009), and not seasonal influenza virus, was detected. Viruses other than influenza virus accounted for the majority of acute respiratory infections. The FTD assay can be easily implemented in general diagnostic laboratories and facilitate the optimization of patient-management schemes.
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The higher co-detection frequency supports previous observations that analytical design and target range may affect co-detection frequency.
“This finding supports previous observations that analytical design and target range may affect co-detection frequency.19”
From Utility and clinical relevance of multiplex PCR panels for diagnosis of central nervous system infections (2026) · cites this paper as [19]
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Gürler, M., Karaoğlu, Ç., Yurtcu, E. E., Us, E., & Karahan, Z. C. (2026) · The Indian Journal of Medical Research · doi:10.25259/ijmr_2107_2025
“19 These variations highlight the need for critical clinical correlation, especially when low-yield or latent viral targets such as HHV-6, EBV, or parvovirus B19 are detected.”
Metadata
- Authors
- Bierbaum, S., Forster, J., Berner, R., Rücker, G., Rohde, G., Neumann-Haefelin, D., Panning, M., & CAPNETZ study group
- Affiliations
- University of Freiburg (DE)
- Maastricht University Medical Centre (NL)
- Corresponding author
- Panning, Marcus
- Venue
- Archives of Virology
- Published
- 2013-10-15
- Volume
- 159
- Issue
- 4
- Pages
- 669-676
- ISSN
- 0003-9012 (ISSN-L)
- 0304-8608
- 1432-8798
- PMC
- PMC7086993
- OpenAlex
- W2043426416
- Cited by
- 42
- References
- 24
- Citations by year
- 2026: 1 · 2025: 3 · 2024: 6 · 2023: 1 · 2022: 3 · 2021: 4 · 2020: 4 · 2019: 1 · 2018: 3 · 2017: 2 · 2016: 6 · 2015: 4 · 2014: 4
- FWCI
- 3.41
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Archives of Virology (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Research Publications (Maastricht University) (publishedVersion, other-oa)
- Research Publications (Maastricht University) (submittedVersion)
- Funders
- Bundesministerium für Bildung und Forschung (01ES0830)
- Topics
- Health Sciences › Medicine › Epidemiology › Respiratory viral infections research
- Health Sciences › Medicine › Epidemiology › Influenza Virus Research Studies
- Health Sciences › Medicine › Infectious Diseases › Viral gastroenteritis research and epidemiology
- Keywords
- respiratory viruses, multiplex real-time PCR, assay sensitivity and specificity, human bocavirus, respiratory syncytial virus, human metapneumovirus, 2009 H1N1 pandemic, viral coinfection, acute respiratory infections, diagnostic test evaluation
- MeSH terms
- Adolescent, Adult, Child, Child, Preschool, Female, Germany, Humans, Infant, Male, Middle Aged, Prospective Studies, Respiratory Tract Infections, Sensitivity and Specificity, Virology, Viruses, Prevalence, Molecular Diagnostic Techniques, Young Adult, Multiplex Polymerase Chain Reaction, Real-Time Polymerase Chain Reaction
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