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Signaling responses of osteoblast cells to hydroxyapatite: the activation of ERK and SOX9
Abstract
The intracellular signaling cascade triggered as a result of the surface chemistry of the bone mineral hydroxyapatite (HA) remains largely unknown. In this study, we found that the ERK signaling molecule is activated in response to HA. Moreover, we have performed the first systemic analysis of expression profiles using microarray technology. Eleven genes, including those involved in calcium regulation and bone matrix formation, showed a greater than 2.0-fold change in expression level in response to HA. Among those genes upregulated by HA was the gene encoding SOX9, whose expression we confirmed by real-time PCR analysis with a 5.7-fold increase in expression. Taken together, our results suggest that the activation of ERK and SOX9 by HA could have important implications for understanding the mechanisms by which cells respond on a molecular level to HA.
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Hydroxyapatite can interact with bone marrow mesenchymal stem cells and enhance BMSC proliferation, differentiation, and mineralization through signaling pathways including ERK/Sox9, BMP/Smad, Wnt/beta-catenin, TGF-beta, and MAPK.
“Hydroxyapatite, a natural apatite mineral, has the capacity to interact with bone marrow mesenchymal stem cells (BMSCs) and enhance BMSC proliferation, differentiation, and mineralization through its action on various signaling pathways, including ERK/Sox9, BMP/Smad, Wnt/β-catenin, TGF-β, and MAPK [64,65,66,67,68].”
From Recent Advances in Silk Fibroin-Based Composites for Bone Repair Applications: A Review (2025) · cites this paper as [64]
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Recent Advances in Silk Fibroin-Based Composites for Bone Repair Applications: A Review
Zhu, S., Zhang, Q., Xu, X., Liu, Z., Cheng, G., Long, D., Cheng, L., & Dai, F. (2025) · Polymers · doi:10.3390/polym17060772 · Cited by 36
“…a natural apatite mineral, has the capacity to interact with bone marrow mesenchymal stem cells (BMSCs) and enhance BMSC proliferation, differentiation, and mineralization through its action on various signaling pathways, including ERK/Sox9, BMP/Smad, Wnt/ β -catenin, TGF-β , and MAPK [64–68].”
Metadata
- Authors
- Song, J.-H., Kim, J.-H., Park, S., Kang, W., Kim, H.-W., Kim, H.-E., & Jang, J.-H.
- Affiliations
- Inha University (KR)
- Seoul National University (KR)
- Corresponding author
- Jang, Jun-Hyeog
- Venue
- Journal of Bone and Mineral Metabolism
- Published
- 2008-02-27
- Volume
- 26
- Issue
- 2
- Pages
- 138-142
- ISSN
- 0914-8779 (ISSN-L)
- 1435-5604
- OpenAlex
- W2089505818
- Cited by
- 39
- References
- 16
- Citations by year
- 2026: 1 · 2025: 2 · 2024: 4 · 2023: 1 · 2022: 3 · 2021: 2 · 2020: 3 · 2019: 3 · 2018: 2 · 2017: 1 · 2016: 2 · 2015: 3 · 2014: 3 · 2013: 4
- FWCI
- 1.10
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Journal of Bone and Mineral Metabolism (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › Bone Tissue Engineering Materials
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Bone and Dental Protein Studies
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bone Metabolism and Diseases
- Keywords
- hydroxyapatite, osteoblasts, ERK signaling, SOX9, intracellular signaling, gene expression profiling, calcium regulation, bone matrix formation
- MeSH terms
- Animals, Cells, Cultured, Enzyme Activation, Gene Expression Regulation, High Mobility Group Proteins, Osteoblasts, Transcription Factors, Signal Transduction, Durapatite, Gene Expression Profiling, MAP Kinase Signaling System, Extracellular Signal-Regulated MAP Kinases, Mice, SOX9 Transcription Factor
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