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Cited by 32

The DOI matches its Crossref record; 80 of the 80 references it cites were found in a registry or an index.

Cloning and characterization of a novel periplasmic heme-transport protein from the human pathogen Pseudomonas aeruginosa

Tong, Yong, Guo, Maolin·2007·JBIC Journal of Biological Inorganic Chemistry
DOI 10.1007/s00775-007-0226-x
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Abstract

Successful iron acquisition plays a crucial role in bacterial virulence. Numerous Gram-negative pathogenic bacteria have developed a novel heme-acquisition system to steal iron from hosts. This system involves a cell-surface heme receptor, a periplasmic heme-transport protein (HTP) and inner-membrane proteins typical for ATP binding cassette transporters. We have cloned the gene encoding a periplasmic HTP from Pseudomonas aeruginosa, overexpressed it in Escherichia coli and purified it as a 33-kDa His-tagged protein. Heme-staining and heme-content assays reveal that the isolated HTP contains approximately 50% heme-bound and apo forms. The heme is noncovalently attached and can be transferred to apomyoglobin in vitro. Electron paramagnetic resonance and UV-vis spectroscopies indicate a five-coordinate, high-spin, ferric heme in HTP. HTP is reduced by dithionite but not by either dithiothreitol or ascorbate. Fluorescence and circular dichroism spectroscopies indicate a well-ordered structure for the HTP and a conformational change upon heme binding to apo-HTP. This was confirmed by limited proteolysis assays. Apo-HTP binds heme or protoporphyrin IX at 1:1 ratio with high affinity (K (d) approximately 1.2 and 14 nM, respectively). A BLASTP search revealed approximately 52 putative bacterial periplasmic heme transporters, which can be grouped into six classes, most of which are associated with pathogenic bacteria. Multiple sequence alignment reveals that these HTPs share low sequ…

Overview

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Registry

Crossref · 10.1007/s00775-007-0226-x

Cites

80 of 80 references verified0 citation errors · 0 not checkable

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Citations

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Metadata

Authors
Tong, Y., & Guo, M.
Venue
JBIC Journal of Biological Inorganic Chemistry
Publisher
Springer Science and Business Media LLC
Published
2007-03-27
Volume
12
Issue
6
Pages
735-750
ISSN
  • 0949-8257
  • 1432-1327
DOI
10.1007/s00775-007-0226-x
Cited by
32
References
80
Language
en
Licence
www.springer.com/tdm (tdm)

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Data sources

Bibliographic data: Semantic Scholar, OpenAlex, Crossref.

Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.