Expression and clinical significance of non-phagocytic cell oxidase 2 and 4 after human traumatic brain injury
Abstract
The goal of this study was to examine NOX2 and NOX4 expression in clinical samples of patients with traumatic brain injury (TBI), and to explore the correlation of NOX2 and NOX4 expression with the severity of injury, duration of injury, and prognosis. Brain samples of 20 TBI patients within 1 cm of the contusion site were collected and grouped based on duration of injury, Glasgow Coma Scale (GCS) and Glasgow Outcome Scale (GOS), and immunofluorescence staining were performed to examine the expression levels of NOX2 and NOX4 in the neurons and astrocytes. We found that the expression level of NOX2 in neurons and positive rate of NOX2 expression in astrocytes peaked at 12-24 and 6-12 h after injury, respectively. The expression level of NOX4 in neurons peaked at 24-48 h, and the positive rate of NOX4 expression gradually increased with prolonged injury. We also found that the higher the GCS score, the lower the expression levels of NOX2 and NOX4 in neurons was, while higher the GCS score, the lower the positive rate of NOX4 expression in astrocytes was and the higher the GOS grade, the lower the positive rate of expression in astrocytes was. In conclusion, NOX2 and NOX4 expressions significantly increase at an early stage after TBI, and abnormal expressions of NOX2 and NOX4 are correlated to patient prognosis to certain extent.
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The activity of NOX2 in glia may track with the severity of TBI in patients.
“The activity of NOX2 in glia may in fact track with the severity of TBI in patients [119].”
From Untangling senescent and damage‐associated microglia in the aging and diseased brain (2023) · cites this paper as [119]
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- Authors
- Li, Z., Tian, F., Shao, Z., Shen, X., Qi, X., Li, H., Wang, Z., & Chen, G.
- Affiliations
- Yangzhou University (CN)
- Qinghai Provincial Peoples Hospital (CN)
- First Affiliated Hospital of Soochow University (CN)
- Corresponding author
- Shen, Xuming
- Chen, Gang
- Venue
- Neurological Sciences
- Published
- 2014-07-31
- Volume
- 36
- Issue
- 1
- Pages
- 61-71
- ISSN
- 1590-1874 (ISSN-L)
- 1590-3478
- OpenAlex
- W1998478345
- Cited by
- 28
- References
- 29
- Citations by year
- 2025: 5 · 2024: 1 · 2023: 4 · 2022: 1 · 2021: 4 · 2020: 3 · 2019: 1 · 2018: 2 · 2017: 3 · 2016: 2 · 2015: 2
- FWCI
- 1.52
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Neurological Sciences (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Neurology › Traumatic Brain Injury and Neurovascular Disturbances
- Health Sciences › Medicine › Epidemiology › Traumatic Brain Injury Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Redox biology and oxidative stress
- Keywords
- traumatic brain injury, NADPH oxidase 2, NOX4, NOX2, astrocytes, neurons, injury severity, prognosis, temporal gene expression, bruising, oxidative stress
- MeSH terms
- NADPH Oxidase 2, NADPH Oxidase 4, Aged, Astrocytes, Brain, Brain Injuries, Female, Fluorescent Antibody Technique, Humans, Male, Membrane Glycoproteins, Middle Aged, Neurons, Time Factors, Glasgow Coma Scale, NADPH Oxidases, Glasgow Outcome Scale
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