The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Prospective Assessment of a New Polymerase Chain Reaction Target (STEVOR) for Imported Plasmodium falciparum Malaria
Abstract
The diagnostic value of a polymerase chain reaction (PCR)-based method for amplifying a new target of repeated genes (STEVOR) in Plasmodium falciparum was prospectively assessed on samples from 210 febrile patients returning from areas endemic for malaria. This method is capable of detecting 0.01 parasites in one microliter of blood. Plasmodium falciparum STEVOR PCR confirmed the results of the thin- and thick-film direct examination method but identified Plasmodium falciparum in four patients in whom direct examination was inconclusive at the species level. Moreover, PCR was positive in two patients with a negative direct examination. Thus, Plasmodium falciparum STEVOR PCR had 100% sensitivity and specificity and could be used in selected parasitology laboratories when expert advice is required.
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“However, most published PCR assays are gel based with (4, 5, 15) or without (2, 3, 9, 23, 25-27, 29, 31, 35, 36) subsequent probe hybridizations”
From Real-Time PCR for Detection and Identification of Plasmodium spp (2005) · cites this paper as [9]
Citations
Cites
Rapid Diagnostic Tests for Malaria Parasites
Moody, A. (2002) · Clinical Microbiology Reviews · doi:10.1128/cmr.15.1.66-78.2002 · Cited by 1,418
Cited by
Real-Time PCR for Detection and Identification of Plasmodium spp
Mangold, K. A., Manson, R. U., Koay, E. S. C., Stephens, L., Regner, M., Thomson, R. B., Peterson, L. R., & Kaul, K. L. (2005) · Journal of Clinical Microbiology · doi:10.1128/jcm.43.5.2435-2440.2005 · Cited by 278
Metadata
- Authors
- D., F., S., B., C., N., R., D., B., M., A., A.-B., Y., H., D., C., & E., C.
- Venue
- European Journal of Clinical Microbiology & Infectious Diseases
- Published
- 2002-09-01
- Volume
- 21
- Issue
- 9
- Pages
- 679-681
- ISSN
- 0934-9723 (ISSN-L)
- 1435-4373
- OpenAlex
- W1976397713
- Cited by
- 20
- References
- 1
- Citations by year
- 2021: 1 · 2019: 2 · 2016: 2 · 2014: 3 · 2012: 2
- FWCI
- 2.44
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- European Journal of Clinical Microbiology & Infectious Diseases (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Malaria Research and Control
- Life Sciences › Immunology and Microbiology › Parasitology › Bird parasitology and diseases
- Health Sciences › Medicine › Microbiology (medical) › Bacterial Identification and Susceptibility Testing
- Keywords
- Plasmodium falciparum, STEVOR, imported malaria, malaria diagnosis, PCR detection, sensitivity and specificity, species identification
- MeSH terms
- Animals, Female, Humans, Male, Plasmodium falciparum, Prospective Studies, Sampling Studies, Sensitivity and Specificity, Travel, DNA, Protozoan, Polymerase Chain Reaction, Malaria, Falciparum, Endemic Diseases
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.