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Bronchial microbiome of severe COPD patients colonised by Pseudomonas aeruginosa
Open accessAbstract
The bronchial microbiome in severe COPD during stability and exacerbation in patients chronically colonised by Pseudomonas aeruginosa (PA), has not been defined. Our objective was to determine the characteristics of the bronchial microbiome of severe COPD patients colonised and not colonised by P. aeruginosa and its changes during exacerbation. COPD patients with severe disease and frequent exacerbations were categorised according to chronic colonisation by P. aeruginosa. Sputum samples were obtained in stability and exacerbation, cultured, and analysed by 16S rRNA gene amplification and pyrosequencing. Sixteen patients were included, 5 of them showing chronic colonisation by P. aeruginosa. Pseudomonas genus had significantly higher relative abundance in stable colonised patients (p = 0.019), but no significant differences in biodiversity parameters were found between the two groups (Shannon, 3 (2-4) vs 3 (2-3), p = 0.699; Chao1, 124 (77-159) vs 140 (115-163), p = 0.364). In PA-colonised patients bronchial microbiome changed to a microbiome similar to non-PA-colonised patients during exacerbations. An increase in the relative abundance over 20 % during exacerbation was found for Streptococcus, Pseudomonas, Moraxella, Haemophilus, Neisseria, Achromobacter and Corynebacterium genera, which include recognised potentially pathogenic microorganisms, in 13 patients colonised and not colonised by P. aeruginosa with paired samples. These increases were not identified by culture in 5 out of 13 participants (38.5 %). Stable COPD patients with severe disease and PA-colonised showed a similar biodiversity to non-PA-colonised patients, with a higher relative abundance of Pseudomonas genus in bronchial secretions. Exacerbation in severe COPD patients showed the same microbial pattern, independently of previous colonisation by P. aeruginosa.
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“With remarkable consistency, all studies report no increase in bacterial burden and no decrease in community diversity during exacerbations.”
From The microbiome and critical illness (2016) · cites this paper as [133]
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“In exacerbation states, membership of the lung bacterial community shifts, often towards enrichment of the Proteobacteria phylum”
From The microbiome and critical illness (2016) · cites this paper as [133]
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The Microbiome and the Respiratory Tract
Dickson, R. P., Erb-Downward, J. R., Martinez, F. J., & Huffnagle, G. B. (2016) · Annual Review of Physiology · doi:10.1146/annurev-physiol-021115-105238 · Cited by 953
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Dickson, R. P., Martinez, F. J., & Huffnagle, G. B. (2014) · The Lancet · doi:10.1016/s0140-6736(14)61136-3 · Cited by 495
Lung microbiome dynamics in COPD exacerbations
Wang, Z., Bafadhel, M., Haldar, K., Spivak, A., Mayhew, D. N., Miller, B. E., Tal‐Singer, R., Johnston, S. L., Ramsheh, M. Y., Barer, M. R., et al. (2016) · European Respiratory Journal · doi:10.1183/13993003.01406-2015 · Cited by 469
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Metadata
- Authors
- Millares, L., Ferrari, R., Gallego, M., Garcia-Nuñez, M., Pérez-Brocal, V., Espasa, M., Pomares, X., Monton, C., Moya, A., & Monsó, E.
- Affiliations
- Universitat de València (ES)
- Centro de Investigación Biomédica en Red de Enfermedades Respiratorias (ES)
- Corporació Sanitària Parc Taulí (ES)
- Universitat Autònoma de Barcelona (ES)
- Corresponding author
- Monsó, E.
- Venue
- European Journal of Clinical Microbiology & Infectious Diseases
- Published
- 2014-01-22
- Volume
- 33
- Issue
- 7
- Pages
- 1101-1111
- ISSN
- 0934-9723 (ISSN-L)
- 1435-4373
- PMC
- PMC4042013
- OpenAlex
- W2069385595
- Cited by
- 136
- References
- 57
- Citations by year
- 2026: 3 · 2025: 4 · 2024: 14 · 2023: 8 · 2022: 16 · 2021: 9 · 2020: 12 · 2019: 14 · 2018: 15 · 2017: 16 · 2016: 13 · 2015: 8 · 2014: 4
- FWCI
- 10.94
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Locations
- European Journal of Clinical Microbiology & Infectious Diseases (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Funders
- Ministerio de Economía y Competitividad (SAF2012, SAF 2012-31187)
- Generalitat Valenciana (Prometeo/2009/092)
- Sociedad Española de Neumología y Cirugía Torácica
- Instituto de Salud Carlos III (Marató de TV3)
- Topics
- Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Chronic Obstructive Pulmonary Disease (COPD) Research
- Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Cystic Fibrosis Research Advances
- Health Sciences › Dentistry › Periodontics › Oral microbiology and periodontitis research
- Keywords
- chronic obstructive pulmonary disease, COPD exacerbations, sputum microbiota, bacterial diversity, potentially pathogenic microorganisms, Streptococcus, Moraxella, Haemophilus, Neisseria, Corynebacterium, Achromobacter
- MeSH terms
- Aged, Aged, 80 and over, Bronchi, DNA, Bacterial, DNA, Ribosomal, Female, Humans, Male, Middle Aged, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S, Sputum, Cohort Studies, Cluster Analysis, Sequence Analysis, DNA, Pulmonary Disease, Chronic Obstructive, Microbiota
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