The DOI matches its Crossref record; 48 of the 48 references it cites were found in a registry or an index.
Polyphenols extracts from Didymosphenia geminata (Lyngbye) Schmidt altered the motility and viability of Daphnia magna
Open accessPublished version of a preprint
Abstract
Abstract The invasive diatom Didymosphenia geminata (Lyngbye) Schmidt, D. geminata has invaded the austral zone of Chile, causing significant ecological, scientific and societal concerns. We aimed to evaluate the viability and motility Daphnia magna (D. magna), as a biosensor for effects of D. geminata. Toxicity assays were performed in dilutions of river water alone (V/V dilution) and in river water contaminated with D. geminata (V/V dilution) or polyphenols extracted from D. geminata under controlled conditions and different time (acute 30 min and 7 hrs). Our results indicated that D. magna was sensitive to increasing concentrations of D. geminata extracts. We observed a 50% (IC50) viability reduction after 24 h of exposure to a 0.023 V/V dilution and the same value when using polyphenols from D. geminata; additionally, this treatment further reduced the motility capacity by 50% after 72 h. The D. magna organisms were acutely responsive, showing a 50% reduction in frequency at 15 min. We conclude that D. magna is sensitive to polyphenols produced by D. geminata in rivers, suggesting potential chronic toxic consequences on several aquatic species following exposure to these diatom substances.
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Citations
Cites
PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENT
Lowry, O., Rosebrough, N., Farr, A. L., & Randall, R. (1951) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)52451-6 · Cited by 317,331 · cited 3×
“Samples were diluted with Folin-Ciocalteu reagent following the protocol described by Lowry (Lowry et al. 1951), for measured protein, but used for polyphenol detection, and the polyphenol absorbance was measured at 517 nm. Finally, total of 12 samples per year were frozen to avoid degradation…”
MethodAre invasive species a major cause of extinctions?
Gurevitch, J., & Padilla, D. (2004) · Trends in Ecology & Evolution · doi:10.1016/j.tree.2004.07.005 · Cited by 1,579 · cited 1×
“A hypolocomotion response to water-born toxicants in D. magna has been described (Huang et al. 2017).”
BackgroundBending the Curve of Global Freshwater Biodiversity Loss: An Emergency Recovery Plan
Tickner, D., Opperman, J. J., Abell, R., Acreman, M., Arthington, A. H., Bunn, S. E., Cooke, S. J., Dalton, J., Darwall, W., Edwards, G., et al. (2020) · BioScience · doi:10.1093/biosci/biaa002 · Cited by 1,241 · cited 1×
“…are noted in the cell lines of native freshwater species, other species or biological models when representing a bioindicator for water quality (Venugopal 2002) and when evaluating whether D. geminata contamination can induce changes in river biota (Cifuentes et al. 2012; Montoya et al. 2012).”
BackgroundStrayer, D. L. (2010) · Freshwater Biology · doi:10.1111/j.1365-2427.2009.02380.x · Cited by 1,189 · cited 2×
“In Austral native freshwater ecosystems, the recent proliferation of Didymosphenia geminata (Lyngbye) Schmidt (D. geminata) has become a significant concern because of the effect on the ecosystem services they provide (Reid and Torres 2014; Strayer 2010; Taylor and Bothwell 2014).”
BackgroundInvasiveness, invasibility and the role of environmental stress in the spread of non-native plants
Alpert, P., Bone, E., & Holzapfel, C. (2000) · Perspectives in Plant Ecology Evolution and Systematics · doi:10.1078/1433-8319-00004 · Cited by 919
Baun, A., Hartmann, N., Grieger, K., & Kusk, K. (2008) · Ecotoxicology · doi:10.1007/s10646-008-0208-y · Cited by 761 · cited 1×
“D. magna, due to its short life cycle, morphological characteristics and reproductive capacity, is a suitable organism for the study of toxins in aquatic environments (Baun et al. 2008; Gerhardt 2007).”
BackgroundRecent mass invasion of the North American Great Lakes by Ponto–Caspian species
Ricciardi, A. P., & MacIsaac, H. J. (2000) · Trends in Ecology & Evolution · doi:10.1016/s0169-5347(99)01745-0 · Cited by 617 · cited 2×
“In a large-scale ecological change in freshwater ecosystems, perturbations to macroscopic organisms are often documented (Ricciardi and MacIsaac 2000), suggesting the possibility thatD.”
BackgroundAlgae displaying the diadinoxanthin cycle also possess the violaxanthin cycle
Lohr, M., & Wilhelm, C. (1999) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.96.15.8784 · Cited by 304 · cited 4×
“Moreover, this pennate microalgae is rich in antioxidants such as polyphenols and pigments including diadinoxanthin (Lohr and Wilhelm 1999), which was also reported as D. geminata polyphenol toxicity on two salmonid species cell lines (Olivares-Ferretti et al. 2019) and Salmo salar spermatozoa…”
BackgroundMethodInfluentialAquatic Behavioral Ecotoxicology—Prospects and Limitations
Gerhardt, A. (2007) · Human and Ecological Risk Assessment An International Journal · doi:10.1080/10807030701340839 · Cited by 221 · cited 1×
“Considerable attention has been paid to this D. geminata problem, as it has invaded more than 187 rivers in many other countries (Blanco and Ector 2009; Gretz et al. 2007).”
BackgroundBiosensors in fish production and quality control.
Venugopal, V. (2002) · Biosensors & bioelectronics · doi:10.1016/s0956-5663(01)00180-4 · Cited by 164 · cited 2×
“These effects are of great interest when considering whether the same effects are noted in the cell lines of native freshwater species, other species or biological models when representing a bioindicator for water quality (Venugopal 2002) and when evaluating whether D.”
Background
Research works printed but not linked: 1
- Gretz MR, Riccio ML, Kiemle SN, Domozych DS, Spaulding SA (2007) Didymosphenia geminata as a nuisance diatom: runaway stalk production results in mats with significant environmental impact. J Phycol 43:16–16
Cited by
Camperio, J., Simonton, J., Paresky, E., Parodi, J., Parodí, J., & Benetti, D. D. (2025) · Sustainability · doi:10.3390/su17051788 · Cited by 35
Self-citationGarralaga, M. P., Lomba, L., Zuriaga, E., Santander, S., & Giner, B. (2022) · Applied Sciences · doi:10.3390/app122211710 · Cited by 12
Messyasz, B., Strugała, M., Łęska, B., Piotrowicz, Z., Treska, E., Tabisz, Ł., & Pankiewicz, R. (2024) · Ecological Indicators · doi:10.1016/j.ecolind.2024.111703 · Cited by 7
Piotrowicz, Z., Łęska, B., Messyasz, B., Tabisz, Ł., & Pankiewicz, R. (2026) · Marine Environmental Research · doi:10.1016/j.marenvres.2026.107922 · Cited by 2
Metadata
- Authors
- Olivares-Ferretti, P., Chavez, V., Hernandez, K., Peredo-Parada, M., & Parodi, J.
- Affiliations
- Universidad de La Frontera (CL)
- Venue
- Aquatic Ecology
- Published
- 2021-08-07
- Volume
- 56
- Issue
- 1
- Pages
- 35-45
- ISSN
- 1386-2588 (ISSN-L)
- 1573-5125
- OpenAlex
- W3188579123
- Cited by
- 4
- References
- 48
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 1 · 2022: 1
- FWCI
- 0.19
- Type
- journal article
- Language
- en
- Open access
- green
- doi.org
- Locations
- Aquatic Ecology (publishedVersion)
- Research Square (acceptedVersion, cc-by)
- Omeka S (Universidad de La Frontera) (submittedVersion, cc-by-nc-nd)
- Funders
- Comisión Nacional de Investigación Científica y Tecnológica
- Topics
- Physical Sciences › Environmental Science › Health, Toxicology and Mutagenesis › Environmental Toxicology and Ecotoxicology
- Physical Sciences › Environmental Science › Environmental Chemistry › Aquatic Ecosystems and Phytoplankton Dynamics
- Physical Sciences › Environmental Science › Ecology › Aquatic Invertebrate Ecology and Behavior
- Keywords
- Didymosphenia geminata, Daphnia magna, polyphenols, bioindicators, aquatic toxicity, motility, cell viability, invasive diatoms, river pollution, acute toxicity, chronic toxicity
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