Matrix metalloproteinases and inflammatory cytokines in oral fluid of patients with chronic generalized periodontitis and various construction materials
Abstract
We present the results of comparative enzyme-linked immunosorbent assay of matrix metalloproteinases MMP-2, MMP-8, MMP-9; IL-1β and IL-6; tissue inhibitors of MMP (TIMP-1, TIMP-2), and TNF-α. The object of study was oral fluid of practically healthy subjects with intact periodontium and patients with chronic generalized periodontitis with various structural materials of dental restorations. It was found that MMP-9 in oral fluid could be considered as a marker of chronic generalized periodontitis irrespective of the presence or absence of metal dental restorations. The level of MMP-8 surpassed the normal only in oral fluid of patients with chronic generalized periodontitis with metal restorations. The correlations between the studied parameters in patients attest to relatively similar regulation of MMP, IL and TIMP secretion in oral fluid in practically healthy subjects with intact periodontium. In patients with inflammatory destructive periodontal diseases with and without metal dental restorations, the correlation coefficients indicate initiation of biochemical cascade accompanied by activation of cytokine production in response to etiological factors. Patients with periodontitis and metal orthodontic structures showed more pronounced reaction. Orthodontic structures made from chromium-cobalt, or chromium-nickel alloys in the oral cavity of these patients increased the levels of MMP-2, IL-1β and IL-6 in oral fluid.
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Metal ceramic prosthetic restorations could induce a more marked deterioration of periodontal tissues.
“demonstrating once again that metal ceramic prosthetic restorations could induce a more marked deterioration of periodontal tissues [35].”
From Potential Impact of Prosthetic Biomaterials on the Periodontium: A Comprehensive Review (2023) · cites this paper as [35]
Enzymes such as resistin, aspartate aminotransferase, alkaline phosphatase and matrix metalloproteases (mainly MMP-2, MMP-8, aMMP-8 and MMP-9) have been implicated in destruction of periodontal tissues.
“even some enzymes such as resistin, aspartate aminotransferase, alkaline phosphatase and matrix metalloproteases (MMPs), mainly MMP-2, MMP-8, aMMP-8 and MMP-9, which have been implicated in the destruction of periodontal tissues [26,35]”
From Potential Impact of Prosthetic Biomaterials on the Periodontium: A Comprehensive Review (2023) · cites this paper as [35]
Patients with periodontitis and with Cr-Co or nickel-chromium (Ni-Cr)-based metal restorations show a more pronounced inflammatory reaction with increased levels of MMP-8, IL-1β, IL-6 and TNF-α compared to patients with periodontal involvement but without prosthetic restorations.
“patients with periodontitis and with Cr-Co or nickel-chromium (Ni-Cr)-based metal restorations show a more pronounced inflammatory reaction with increased levels of MMP-8, IL-1β, IL-6 and TNF-α compared to patients with periodontal involvement but without prosthetic restorations”
From Potential Impact of Prosthetic Biomaterials on the Periodontium: A Comprehensive Review (2023) · cites this paper as [35]
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“Metal and metal-ceramic prosthetic restorations with Cr-Co and Ni-Cr alloys MMP-2 MMP-8 MMP-9 IL-1β IL-6 TNF-α TIMP-1 TIMP-2 GCF ELISA Patients with prosthetic restorations and periodontitis have increased levels of TNF- α, MMP-8, IL-1β and IL-6 compared to patients without prosthetic restorations [35]”
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XRONİK GENERALİZASİYA ETMİŞ PARODONTİTİN ETİOPATOGENEZİNİN MÜASİR ASPEKTLƏRİ
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- Authors
- Kushlinskii, N. E., Solovykh, E. A., Karaoglanova, T. B., Boyar, U., Gershtein, E. S., Troshin, A. A., Maksimovskaya, L. N., & Yanushevich, O. O.
- Affiliations
- Russian University of Medicine (RU)
- Venue
- Bulletin of Experimental Biology and Medicine
- Published
- 2012-05
- Volume
- 153
- Issue
- 1
- Pages
- 72-76
- ISSN
- 0007-4888 (ISSN-L)
- 1573-8221
- OpenAlex
- W1965739562
- Cited by
- 14
- References
- 13
- Citations by year
- 2025: 1 · 2024: 2 · 2023: 1 · 2022: 1 · 2021: 3 · 2020: 4 · 2018: 1 · 2013: 1
- FWCI
- 0.39
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Bulletin of Experimental Biology and Medicine (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Dentistry › Periodontics › Oral microbiology and periodontitis research
- Health Sciences › Medicine › Urology › Periodontal Regeneration and Treatments
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Protease and Inhibitor Mechanisms
- Keywords
- chronic generalized periodontitis, matrix metalloproteinases, MMP-9, MMP-8, MMP-2, tissue inhibitors of metalloproteinases, inflammatory cytokines, oral fluid, orthodontic appliances, cobalt-chromium alloy, nickel-chromium alloy, IL-1β, interleukin-6, TNF-α, periodontal biomarkers
- MeSH terms
- Adolescent, Adult, Enzyme-Linked Immunosorbent Assay, Female, Gingival Crevicular Fluid, Humans, Male, Middle Aged, Tumor Necrosis Factor-alpha, Interleukin-6, Cytokines, Tissue Inhibitor of Metalloproteinase-1, Tissue Inhibitor of Metalloproteinase-2, Matrix Metalloproteinase 9, Matrix Metalloproteinases, Matrix Metalloproteinase 8, Chronic Periodontitis, Young Adult
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