Isolation of induced pluripotent cells from stromal liver cells of patients with alcoholic cirrhosis
Abstract
Stromal liver cells obtained from liver biopsy specimens of a patient with alcoholic cirrhosis can proliferate for a long time in culture passing more than 30 passages. In the course of culturing from early to late passages, acceleration of cell proliferation, decrease of the expression of some markers, and loss of hepatogenic differentiation potential were observed. On passage 30, induced pluripotent stem cells were obtained from these cells and comparative analysis of adipogenic and hepatic differentiation potencies of these cells and original liver stromal cells was performed. Induced pluripotent stem cells differentiated into both directions more efficiently and more rapidly than initial cells. Under conditions of hepatic differentiation, liver stromal cells started to express markers of definitive endoderm, but not markers of immature/mature hepatocytes, whereas induced pluripotent stem cells consistently expressed markers of definitive endoderm, immature/mature hepatocytes.
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The absence of the Fc domain and the small size of antibody fragments results in a shorter half-life compared to full-length antibodies.
“On the other hand, the absence of the Fc domain and the small size results in a shorter half-life compared to full-length antibodies [2].”
From Evolution of Escherichia coli Expression System in Producing Antibody Recombinant Fragments (2020) · cites this paper as [2]
Smaller antibody fragments have pharmacokinetic advantages over whole antibodies including better tissue penetration, faster clearance for imaging, and generally lower immunogenicity.
“Unlike conventional immunoglobulins, these smaller biomolecules take several pharmacokinetics advantages over whole antibodies including better penetration into tissues, faster clearance for imaging purposes and generally lower immunogenicity.”
From Evolution of Escherichia coli Expression System in Producing Antibody Recombinant Fragments (2020) · cites this paper as [2]
Cites
Human hepatocyte transplantation: current experience and future challenges
Dhawan, A., Puppi, J., Hughes, R. D., & Mitry, R. R. (2010) · Nature Reviews Gastroenterology & Hepatology · doi:10.1038/nrgastro.2010.44 · Cited by 362 · see citing works
A Comparative View on Human Somatic Cell Sources for iPSC Generation
Raab, S., Klingenstein, M., Liebau, S., & Linta, L. (2014) · Stem Cells International · doi:10.1155/2014/768391 · Cited by 263 · see citing works
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Evolution of Escherichia coli Expression System in Producing Antibody Recombinant Fragments
Sandomenico, A., Sivaccumar, J. P., & Ruvo, M. (2020) · International Journal of Molecular Sciences · doi:10.3390/ijms21176324 · Cited by 134 · see citing works
“On the other hand, the absence of the Fc domain and the small size results in a shorter half-life compared to full-length antibodies [2].”
Background
- Authors
- Kholodenko, I. V., Kholodenko, R. V., Manukyan, G. V., Lupatov, A. Y., & Yarygin, K. N.
- Affiliations
- Institute of Biomedical Chemistry (RU)
- Russian Academy of Sciences (RU)
- Russian Scientific Center of Surgery (RU)
- Corresponding author
- Kholodenko, I. V.
- Venue
- Bulletin of Experimental Biology and Medicine
- Published
- 2017-08
- Volume
- 163
- Issue
- 4
- Pages
- 535-541
- ISSN
- 0007-4888 (ISSN-L)
- 1573-8221
- OpenAlex
- W2753404988
- Cited by
- 5
- References
- 2
- Citations by year
- 2024: 1 · 2021: 1 · 2020: 2 · 2019: 1
- FWCI
- 0.34
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Bulletin of Experimental Biology and Medicine (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
- Health Sciences › Medicine › Genetics › Mesenchymal stem cell research
- Health Sciences › Medicine › Hepatology › Liver physiology and pathology
- Keywords
- induced pluripotent stem cells, alcoholic cirrhosis, hepatocyte differentiation, adipogenesis, definitive endoderm, hepatocyte markers, cell proliferation, liver biopsy
- MeSH terms
- Cell Differentiation, Humans, Liver, Liver Cirrhosis, Alcoholic, Stromal Cells, Hepatocytes, Cell Proliferation, Induced Pluripotent Stem Cells
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