Student or educator? Sign up with your school email and get 6 months of Pro free.
CoChat
Sign Up
Cited by 23

The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.

Telomerase activity in HeLa cervical carcinoma cell line proliferation

Ivanković, Milena, Ćukušić, Andrea, Gotić, Ivana, Škrobot, Nikolina, Matijašić, Mario, Polančec, Denis, Rubelj, Ivica·2007·Biogerontology
DOI 10.1007/s10522-006-9043-9
Open this paper in CoChat — free account

Abstract

Normal human somatic cells in culture have a limited dividing potential. This is due to DNA end replication problem, whereby telomeres shorten with each subsequent cell division. When a critical telomere length is reached cells enter senescence. To overcome this problem, immortal HeLa cell line express telomerase, an enzyme that prevents telomere shortening. Although immortal, the existence of non-dividing cells that do not incorporate (3)H-thymidine over 24 h of growth has been well documented in this cell line. Using DiI labeling and high-speed cell sorting, we have separated and analyzed fractions of HeLa cells that divided vigorously as well as those that cease divisions over several days in culture. We also analyzed telomerase activity in separated fractions and surprisingly, found that the fraction of cells that divided 0-1 time over 6 days in culture have several times higher endogenous telomerase activity than the fastest dividing fraction. Additionally, the non-growing fraction regains an overall high labeling index and low SA-beta-Gal activity when subcultured again. This phenomenon should be considered if telomerase inhibition is to be used as an approach to cancer therapy. In this paper we also discuss possible molecular mechanisms that underlie the observed results.

Overview

Integrity signals

Registry

Crossref · 10.1007/s10522-006-9043-9

Cites

2 of 2 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Verification

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Citations

Cites

Research works printed but not linked: 32

  1. Bryan TM, Englezou A, Gupta J, Bacchetti S, Reddel RR (1995) Telomere elongation in immortal human cells without detectable telomerase activity. EMBO J 14:4240–4248 · DOI · Paper page
  2. Bryan TM, Englezou A, Dunham M, Reddel RR (1998) Telomere length dynamics in telomerase-positive immortal human cell populations. Exp Cell Res 239:370–378 · DOI
  3. Campisi J (1992) Gene expression in quiescent and senescent fibroblasts. Ann NY Acad Sci 21:195–201 · DOI
  4. Campisi J (2000) Cancer, aging and cellular senescence. In Vivo 14:183–188
  5. Corey RD (2002) Telomerase inhibition, oligonucleotides, and clinical trials. Oncogene 21:631–637 · DOI
  6. Counter CM, Avilion AA, LeFeuvre CE, Stewart NG, Greider CW, Harley CB, Bacchetti S (1992) Telomere shortening associated with chromosome instability is arrested in immortal cell which express telomerase activity. EMBO J 11:1921–1929 · DOI · Paper page
  7. Cristofalo VJ, Pignolo RJ, Rotenberg MO (1992) Molecular changes with in vitro cellular senescence. Ann NY Acad Sci 663:187–194 · DOI
  8. Dimri GP, Lee X, Basile G, Acosta M, Scott G, Roskelley C, Medrano EE, Linskens M, Rubelj I, Pereira-Smith OM (1995) A biomarker that identifies senescent human cells in culture and aging skin in vivo. Proc Natl Acad Sci USA 92:9363–9367 · DOI · Paper page
  9. Feng J, Funk WD, Wang SS, Weinrich SL, Avilion AA, Chiu CP, Adams RR, Chang E, Allsopp RC, Yu J, Le S, West MD, Harley CB, Andrews WH, Greider CW, Villeponteau B (1995) The RNA component of human telomerase. Science 269:1236–1241 · DOI · Paper page
  10. Ferenac M, Polancec D, Huzak M, Pereira-Smith OM, Rubelj I (2005) Early-senescing human skin fibroblasts do not demonstrate accelerated telomere shortening. J Gerontol A Biol Sci Med Sci 60:820–829 · DOI
  11. Goodwin EC, DiMaio D (2001) Induced senescence in HeLa cervical carcinoma cells containing elevated telomerase activity and extended telomeres. Cell Growth Differ 11:525–534
  12. Gorbunova V, Seluanov A, Pereira-Smith OM (2003) Evidence that high telomerase activity may induce a senescent-like growth arrest in human fibroblasts. J Biol Chem 287:7692–7698 · DOI
  13. Harley CB, Futcher AB, Greider CW (1990) Telomeres shorten during ageing of human fibroblast. Nature 345:458–460 · DOI · Paper page
  14. Hayflick L (1965) The limited in vitro lifetime of human diploid cell strains. Exp Cell Res 37:614–636 · DOI · Paper page
  15. Hayflick L, Moorhead PS (1961) The limited in vitro lifetime of human diploid cell strains. Exp Cell Res 25:585–621 · DOI · Paper page
  16. Kim NW, Piatyszek MA, Prowse KR, Harley CB, West MD, Ho PL, Coviello GM, Wright WE, Weinrich SL, Shay JW (1994) Specific association of human telomerase activity in immortal cells and cancer. Science 266:2011–2015 · DOI · Paper page
  17. Ledley FD, Soriano HE, O’Malley BW Jr, Lewis D, Darlington GJ, Finegold M (1992) DiI as a marker for cellular transplantation into solid organs. Biotechniques 13:580–587
  18. Makarov VL, Hirose Y, Langmore JP (1997) Long G tails at both ends of human chromosomes suggest a C strand degradation mechanism for telomere shortening. Cell 88:657–666 · DOI
  19. Martinez AO, Norwood TH, Prothero JW, Martin GM (1978) Evidence for clonal attenuation of growth potential in HeLa cells. In Vitro 14:996–1002 · DOI
  20. Matsumura T, Pfendt EA, Hayflick L (1979) DNA synthesis in the human diploid cell strain WI-38 during in vitro aging: an autoradiography study. J Gerontol 34:323–327 · DOI
  21. Neumann AA, Reddel RR (2002) Telomere maintenance and cancer—look, no telomerase. Nat Rev Cancer 2:879–884 · DOI
  22. Olovnikov AM (1973) The incomplete copying of template margin in enzymatic synthesis of polynucleotides and biological significance of the phenomenon. J Theor Biol 41:181–190 · DOI
  23. Pereira-Smith OM, Smith JR (1981) Expression of SV40 T antigen in finite life-span hybrids of normal and SV40-transformed fibroblasts. Somatic Cell Genet 7:411–421 · DOI
  24. Roninson IB (2003) Tumor cell senescence in cancer treatment. Can Res 63:2705–2715
  25. Rubelj I, Venable SF, Lednicky J, Butel JS, Bilyeu T, Darlington G, Surmacz E, Campisi J, Pereira-Smith OM (1997) Loss of T-antigen sequences allows SV40-transformed human cells in crisis to acquire a senescent-like phenotype. J Gerontol A Biol Sci Med Sci 52:229–234 · DOI
  26. Rubelj I, Huzak M, Brdar B, Pereira-Smith OM (2002) A single stage mechanism controls replicative senescence through Sudden Senescence Syndrome. Biogerontology 3:213–222 · DOI
  27. Saretzki G, Von Zglinicki T (2002) Replicative aging, telomeres and oxidative stress. Ann NY Acad Sci 959:24–29 · DOI
  28. Shay JW, Bacchetti S (1997) A survey of telomerase activity in human cancer. Eur J Cancer 33:787–791 · DOI · Paper page
  29. Te Poele RH, Ohorkov AL, Jardine L, Cummings J, Joel SP (2002) DNA damage is able to induce senescence in tumor cells in vitro and in vivo. Cancer Res 62:1876–1883
  30. Touissaint O, Medrano EE, Von Zglinicki T (2000) Cellular and molecular mechanisms of stress-induced premature senescence (SIPS) of human diploid fibroblasts and melanocytes. Exp Gerontol 35:927–945 · DOI · Paper page
  31. Von Zglinicki T, Nilsson E, Docke WD, Brunk UT (1995) Lipofuscin accumulation and ageing of fibroblasts. Gerontology 41:95–108 · DOI
  32. Wright WE, Piatyszek MA, Rainey WE, Byrd W, Shay JW (1996) Telomerase activity in human germline and embryonic tissues and cells. Dev Genet 18:173–179 · DOI · Paper page

Cited by

See all 23

Metadata

Authors
Ivanković, M., Ćukušić, A., Gotić, I., Škrobot, N., Matijašić, M., Polančec, D., & Rubelj, I.
Venue
Biogerontology
Publisher
Springer Science and Business Media LLC
Published
2006-09-06
Volume
8
Issue
2
Pages
163-172
ISSN
  • 1389-5729
  • 1573-6768
DOI
10.1007/s10522-006-9043-9
Cited by
23
References
2
Language
en
Licence

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review