A comparison between osteogenic differentiation of human unrestricted somatic stem cells and mesenchymal stem cells from bone marrow and adipose tissue
Abstract
To evaluate the potential of three stem cells for cell therapy and tissue engineering applications, the biological behavior and osteogenic capacity of the newly introduced cord-blood-derived, unrestricted somatic stem cells (USSC) were compared with those of mesenchymal stem cells isolated from bone marrow (BM-MSC) and adipose tissue (AT-MSC). There was no significant difference between the rates of proliferation of the three stem cells. During osteogenic differentiation, alkaline phosphatase (ALP) activity peaked on day 7 in USSC compared to BM-MSC which showed the maximum value of ALP activity on day 14. However, BM-MSC had the highest ALP activity and mineralization during osteogenic induction. In addition, AT-MSC showed the lowest capacity for mineralization during differentiation and had the lowest ALP activity on days 7 and 14. Although AT-MSC expressed higher levels of collagen type I, osteonectin and BMP-2 in undifferentiated state, but these genes were expressed higher in BM-MSC during differentiation. BM-MSC also expressed higher levels of ALP, osteocalcin and Runx2 during induction. Taking together, BM-MSC showed the highest capacity for osteogenic differentiation and hold promising potential for bone tissue engineering and cell therapy applications.
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Some studies suggest that BM-MSCs exhibit greater osteogenic capacity than ASCs.
“Some suggest that BM-MSCs exhibit greater osteogenic capacity [58,59,60,61,62], while others propose that ASCs have equal or superior potential [58,63,64,65,66].”
From Mesenchymal Stem/Stromal Cells: Immunomodulatory and Bone Regeneration Potential after Tumor Excision in Osteosarcoma Patients (2023) · cites this paper as [59]
Studies comparing the osteogenic potential of human BM-MSCs and ASCs have yielded mixed results.
“Regarding bone tissue engineering, studies comparing the osteogenic potential of human BM-MSCs and ASCs have yielded mixed results.”
From Mesenchymal Stem/Stromal Cells: Immunomodulatory and Bone Regeneration Potential after Tumor Excision in Osteosarcoma Patients (2023) · cites this paper as [59]
Cites
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Adipose-derived and bone marrow mesenchymal stem cells: A donor-matched comparison
Mohamed-Ahmed, S., Fristad, I., Lie, S. A., Suliman, S., Mustafa, K., Vindenes, H., & Idris, S. B. (2018) · Stem Cell Research & Therapy · doi:10.1186/s13287-018-0914-1 · Cited by 515 · see citing works
The Bone Marrow-Derived Stromal Cells: Commitment and Regulation of Adipogenesis
Tencerova, M., & Kassem, M. (2016) · Frontiers in Endocrinology · doi:10.3389/fendo.2016.00127 · Cited by 149 · see citing works
Ragni, E., Montemurro, T., Montelatici, E., Lavazza, C., Viganò, M., Rebulla, P., Giordano, R., & Lazzari, L. (2013) · Experimental Cell Research · doi:10.1016/j.yexcr.2013.04.002 · Cited by 48 · see citing works
Baron, M., Drohat, P., Crawford, B., Hornicek, F. J., Best, T. M., & Kouroupis, D. (2023) · Bioengineering · doi:10.3390/bioengineering10101187 · Cited by 19 · see citing works
- Authors
- Shafiee, A., Seyedjafari, E., Soleimani, M., Ahmadbeigi, N., Dinarvand, P., & Ghaemi, N.
- Affiliations
- Stem Cell Technology Research Center (IR)
- University of Tehran (IR)
- Tarbiat Modares University (IR)
- Corresponding author
- Soleimani, Masoud
- Venue
- Biotechnology Letters
- Published
- 2011-02-02
- Volume
- 33
- Issue
- 6
- Pages
- 1257-1264
- ISSN
- 0141-5492 (ISSN-L)
- 1573-6776
- OpenAlex
- W1982923563
- Cited by
- 163
- References
- 1
- Citations by year
- 2026: 3 · 2025: 7 · 2024: 4 · 2023: 5 · 2022: 9 · 2021: 9 · 2020: 14 · 2019: 9 · 2018: 12 · 2017: 13 · 2016: 9 · 2015: 25 · 2014: 13 · 2013: 19 · 2012: 8
- FWCI
- 7.10
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Biotechnology Letters (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Genetics › Mesenchymal stem cell research
- Physical Sciences › Engineering › Biomedical Engineering › Bone Tissue Engineering Materials
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
- Keywords
- osteogenic differentiation, unrestricted somatic stem cells, mesenchymal stem cells, bone marrow-derived stem cells, adipose-derived stem cells, cord blood-derived stem cells, alkaline phosphatase activity, mineralization, osteocalcin, Runx2, BMP-2, SPARC, type I collagen, bone tissue engineering, cell therapy
- MeSH terms
- Adipose Tissue, Alkaline Phosphatase, Bone Marrow Cells, Calcium, Cell Differentiation, Cell Separation, Fetal Blood, Humans, Osteogenesis, RNA, Messenger, Stem Cells, Osteocalcin, Osteonectin, Gene Expression, Tissue Engineering, Collagen Type I, Cell Proliferation, Core Binding Factor Alpha 1 Subunit, Bone Morphogenetic Protein 2, Mesenchymal Stem Cells
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