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Ultra-low carbon dioxide partial pressure improves the galactosylation of a monoclonal antibody produced in Chinese hamster ovary cells in a bioreactor
Abstract
Objective To explore the influence of ultra-low carbon dioxide partial pressure (pCO 2 ) on the monoclonal antibody (mAb) N-glycosylation profile in Chinese hamster ovary (CHO) cell culture. Results In fed-batch bioreactor cultures, lowering the pCO 2 in the medium ( 2 was approximately 27.45 ± 2.13%, noticeably higher than that observed under normal pCO 2 (21.36 ± 1.66%) at harvest. However, all of the relevant intracellular nucleotide sugar concentrations were dramatically decreased to approximately 50% of the levels found under normal pCO 2 on day 7. Real-time PCR revealed that the upregulation of galactosylation-related glycosyltransferase genes and substrate transporter genes played a critical role in the improved galactosylation under the ultra-low pCO 2 condition. Conclusions In the bioreactor culture processes, ultra-low pCO 2 demonstrated a positive effect on mAb galactosylation.
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Lower pCO2 results in a shift away from G0F and G0 and toward G2F.
“lower pCO2 results in a shift away from G0F and G0 and toward G2F”
From Dynamic pH profiles drive higher cell‐specific and volumetric productivity (2026) · cites this paper as [5]
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Metadata
- Authors
- Wang, C., Wang, J., Chen, M., Fan, L., Zhao, L., & Tan, W.-S.
- Affiliations
- East China University of Science and Technology (CN)
- Corresponding author
- Zhao, Liang
- Venue
- Biotechnology Letters
- Published
- 2018-06-19
- Volume
- 40
- Issue
- 8
- Pages
- 1201-1208
- ISSN
- 0141-5492 (ISSN-L)
- 1573-6776
- OpenAlex
- W2809294243
- Cited by
- 11
- References
- 14
- Citations by year
- 2025: 3 · 2024: 3 · 2022: 1 · 2021: 1 · 2020: 3
- FWCI
- 0.36
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Biotechnology Letters (publishedVersion)
- PubMed (publishedVersion)
- Funders
- The National Natural Science Foundation of China (21106045)
- National Natural Science Foundation of China (21406066)
- the National High Technology Research and Development Program of China (2012AA02A303)
- the National Science and Technology Major Project (2013ZX10004003-003-003)
- the Development Funds of Shanghai Zhangjiang National Independent Innovation Demonstration Zone (ZJ2015-ZD-002)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Viral Infectious Diseases and Gene Expression in Insects
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Glycosylation and Glycoproteins Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein purification and stability
- Keywords
- monoclonal antibodies, N-glycosylation, galactosylation, CHO cell culture, fed-batch bioreactor, nucleotide sugar metabolism, recombinant antibody production
- MeSH terms
- Animals, Antibodies, Monoclonal, Biotechnology, Carbon Dioxide, Cricetulus, Culture Media, Female, Galactose, Glycosylation, Immunologic Factors, Partial Pressure, CHO Cells, Bioreactors
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