Cited by 76Environmental Toxicology and EcotoxicologyAquaculture disease management and microbiotaAquaculture Nutrition and Growth

Alterations in serum electrolytes, antioxidative enzymes and haematological parameters of Labeo rohita on short-term exposure to sublethal dose of nitrite

Ciji, Alexander, Sahu, N. P., Pal, A. K., Dasgupta, S., Akhtar, M. S.·2012·Fish Physiology and Biochemistry

Abstract

An experiment was conducted to study the effects of short-term exposure to sublethal levels of nitrite on electrolyte regulation, antioxidative enzymes and haematological parameters in Labeo rohita juveniles. The fishes were exposed to graded levels of nitrite (0-15 mg l(-1)) for different duration (0, 12, 24, 48 and 96 h). The 96-h LC(50) value for L. rohita (avg. wt, 66.5 ± 0.5 g) was found to be 11.28 mg l(-1). Activities of antioxidative enzymes (catalase and superoxide dismutase), acetylcholine esterase (AChE) and methaemoglobin reductase, serum electrolytes (sodium, potassium and chloride), haematological parameters and blood glucose level significantly varied (P < 0.05) in a dose-dependent manner. With increasing nitrite concentration and exposure period, a progressive reduction in the total erythrocyte count and haemoglobin were observed. With increase in nitrite concentration, a significant (P < 0.05) increase in activities was evidenced in catalase and superoxide dismutase in liver as well as gill, methaemoglobin reductase in blood, while progressive decline in AChE activity in brain was recorded. The serum sodium and chloride content showed a progressive decline, while potassium showed an increasing trend upon increase in nitrite concentration. The serum K(+) and Cl(-) after 96-h exposure demonstrated a linear relationship (Y = 0.221x + 2.542, R (2) = 0.938, P < 0.01 and Y = -5.760x + 129.5, R (2) = 0.952, P < 0.01, respectively) with nitrite concentrations. This study revealed that nitrite exposure causes alteration in all measured tissue enzymes, serum electrolytes and haematological parameters.

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Crossref · 10.1007/s10695-012-9623-3

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Authors
Ciji, A., Sahu, N. P., Pal, A. K., Dasgupta, S., & Akhtar, M. S.
Affiliations
  • Central Institute of Fisheries Education (IN)
  • Directorate of Coldwater Fisheries Research (IN)
Corresponding author
Ciji, Alexander
Venue
Fish Physiology and Biochemistry
Published
2012-03-03
Volume
38
Issue
5
Pages
1355-1365
ISSN
  • 0920-1742 (ISSN-L)
  • 1573-5168
OpenAlex
W1964987340
Cited by
75
References
56
Citations by year
2026: 1 · 2025: 7 · 2024: 10 · 2023: 1 · 2022: 4 · 2021: 8 · 2020: 7 · 2019: 9 · 2018: 7 · 2017: 6 · 2016: 2 · 2015: 2 · 2014: 7 · 2013: 3 · 2012: 1
FWCI
2.88
Type
journal article
Language
en
Open access
closed
Locations
  • Fish Physiology and Biochemistry (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Physical Sciences › Environmental Science › Health, Toxicology and Mutagenesis › Environmental Toxicology and Ecotoxicology
  • Life Sciences › Immunology and Microbiology › Immunology › Aquaculture disease management and microbiota
  • Life Sciences › Agricultural and Biological Sciences › Aquatic Science › Aquaculture Nutrition and Growth
Keywords
nitrite toxicity, Labeo rohita, sublethal exposure, serum electrolytes, antioxidant enzymes, catalase, superoxide dismutase, acetylcholinesterase, methemoglobin reductase, hematological parameters, blood glucose, LC50, sodium, potassium, chloride
MeSH terms
Acetylcholinesterase, Animals, Antioxidants, Blood Glucose, Catalase, Cyprinidae, Dose-Response Relationship, Drug, Drug Administration Schedule, Electrolytes, Hydrocortisone, Lethal Dose 50, Nitrites, Superoxide Dismutase, Gene Expression Regulation, Enzymologic, Cytochrome-B(5) Reductase

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