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Nuclear DNA content estimations in wild olive (Olea europaea L. ssp. europaea var. sylvestris Brot.) and Portuguese cultivars of O. europaea using flow cytometry
Abstract
Olive tree (Olea europaea L.) is an economically important woody fruit crop widely distributed in the Mediterranean regions. In this work the genome size of six Portuguese cultivars of olive (O. europaea ssp. europaea var. europaea) and wild olive (O. europaea spp. europaea var. sylvestris) was estimated for the first time. The nuclear DNA content of O. europaea cultivars ranged between 2.90 ± 0.020 pg/2C and 3.07 ± 0.018 pg/2C and the genome size of wild olive was estimated as 3.19 ± 0.047 pg/2C DNA. These results suggest a low intraspecific variation at least among the studied cultivars and between them and wild olive. This is not in accordance with previous results in some Italian cultivars where high genome size heterogeneity was found. The methodology presented here seems appropriate for genome size estimations within this genus and opens good perspectives for a large screening of estimation of nuclear DNA content among O. europaea cultivars and Olea species that could clarify this issue.
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In this study, DNA amounts were similar to previously measured olive DNA amounts (2.90-3.07 pg).
“In this study, DNA amounts were similar to olives measured previously (2.90–3.07 pg) [7].”
For wild olive, 2C DNA content was 3.19 ± 0.047 pg/2C.
“Loureiro et al. [7] reported that 2C DNA content of O. europaea ssp. europaea var. europaea ranged between 2.90 ± 0.020 pg/2C and 3.07 ± 0.018 pg/2C and for wild olive, it was 3.19 ± 0.047 pg/2C.”
Interfering phenolic compounds are genotype-dependent and normally micropropagated plants accumulate higher amounts of cytosolic compounds which do not allow proper PI staining resulting in lower DNA amount.
“These interfering phenolic compounds are genotype-dependent and normally micropropagated plants accumulate higher amounts of cytosolic compounds which do not allow proper PI staining resulting in lower DNA amount [7].”
2C DNA content of O. europaea ssp. europaea var. europaea ranged between 2.90 ± 0.020 pg/2C and 3.07 ± 0.018 pg/2C.
“Loureiro et al. [7] reported that 2C DNA content of O. europaea ssp. europaea var. europaea ranged between 2.90 ± 0.020 pg/2C and 3.07 ± 0.018 pg/2C and for wild olive, it was 3.19 ± 0.047 pg/2C.”
DNA amount fluctuation might be attributed to cytosolic compounds like phenols causing tannic acid effect as a result of interference with DNA-PI staining.
“DNA amount fluctuation might be attributed to the cytosolic compounds like phenols causing tannic acid effect as a result of interference with DNA-PI staining since there is an abundant amount of such materials in woody plants [7].”
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Research works printed but not linked: 19
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Metadata
- Authors
- Loureiro, J., Rodriguez, E., Costa, A., & Santos, C.
- Affiliations
- University of Aveiro (PT)
- Corresponding author
- Loureiro, João
- Venue
- Genetic Resources and Crop Evolution
- Published
- 2006-11-29
- Volume
- 54
- Issue
- 1
- Pages
- 21-25
- ISSN
- 0925-9864 (ISSN-L)
- 1573-5109
- OpenAlex
- W2035007987
- Cited by
- 67
- References
- 0
- Citations by year
- 2026: 1 · 2025: 2 · 2024: 1 · 2023: 2 · 2022: 1 · 2021: 3 · 2020: 3 · 2019: 3 · 2018: 3 · 2017: 5 · 2016: 10 · 2015: 3 · 2014: 6 · 2013: 5 · 2012: 2
- FWCI
- 2.86
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Genetic Resources and Crop Evolution (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Chromosomal and Genetic Variations
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetic Mapping and Diversity in Plants and Animals
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetic diversity and population structure
- Keywords
- olive, Olea europaea, wild olive, Olea europaea var. sylvestris, genome size, nuclear DNA content, intraspecific variation, flow cytometry
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