The DOI matches its Crossref record; 22 of the 22 references it cites were found in a registry or an index.
Evaluating low-cost substrates for Crypthecodinium cohnii lipids and DHA production, by flow cytometry
Open accessAbstract
Crypthecodinium cohnii growth was studied on pure carbon sources (glucose, acetate, glycerol) and low-cost complex carbon sources (sugarcane molasses, crude glycerol and vinegar effluent) for lipid and DHA production. Among the pure substrates, glucose induced the highest lipid content (14.75% w/w DCW) and DHA content (7.14 mg g −1 DCW). Among the low-cost substrates, the highest lipid and DHA content were observed for the crude glycerol assay (14.7% w/w DCW and 6.56 mg g −1 , respectively). Molasses induced the highest proportion of DHA of total fatty acids (49.58% w/w TFA) among all the substrates studied. Flow cytometric analysis revealed that the vinegar effluent induced the highest proportion of C. cohnii cells with injured membrane (92.8%). These results foresee the possibility of using these low-cost substrates at a larger scale for C. cohnii DHA and biodiesel production, aiming at zero wastes and process costs reduction.
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“Flow cytometry methods for monitoring lipid content, enzymatic activity, cell viability, and cell number in microbial biofuel production have been reviewed [238].”
From Multi-Sensing Monitoring of the Microalgae Biomass Cultivation Systems for Biofuels and Added Value Products Synthesis—Challenges and Opportunities (2025) · cites this paper as [238]
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Cited by
Dębowski, M., Kazimierowicz, J., & Zieliński, M. (2025) · Applied Sciences · doi:10.3390/app15137324 · Cited by 30
Metadata
- Authors
- Taborda, T., Moniz, P., Reis, A., & da Silva, T. L.
- Affiliations
- National Laboratory of Energy and Geology (PT)
- Venue
- Journal of Applied Phycology
- Published
- 2020-10-26
- Volume
- 33
- Issue
- 1
- Pages
- 263-274
- ISSN
- 0921-8971 (ISSN-L)
- 1573-5176
- OpenAlex
- W3096679145
- Cited by
- 19
- References
- 22
- Citations by year
- 2026: 2 · 2025: 5 · 2024: 3 · 2023: 2 · 2022: 4 · 2021: 3
- FWCI
- 0.44
- Type
- journal article
- Language
- en
- Open access
- green
- hdl.handle.net
- Licence
- cc-by
- www.springer.com/tdm (tdm)
- www.springer.com/tdm (vor)
- Locations
- Journal of Applied Phycology (publishedVersion)
- Portuguese National Funding Agency for Science, Research and Technology (RCAAP Project by FCT) (submittedVersion, cc-by)
- Funders
- Fundação para a Ciência e a Tecnologia (PTDC/EAM-AMB/30169/2017)
- Horizon 2020 Framework Programme ((BBRI)-LISBOA-01-0145-FEDER-022059)
- Topics
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
- Keywords
- Crypthecodinium cohnii, docosahexaenoic acid, low-cost substrates, crude glycerol, sugarcane molasses, glucose, acetate, glycerol, membrane damage, fatty acid composition, biodiesel feedstock, waste valorization
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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