Disruption of the GPI Protein-Encoding Gene IFF4 of Candida albicans Results in Decreased Adherence and Virulence
Abstract
Candida albicans is the most important cause of systemic fungal infection in immunocompromised humans. Candidiasis is often initiated by the adherence and the colonization of inert surfaces such as peripheral venous catheters, central catheters, prosthetic cardiac valves, and other prostheses. We have studied the early stage of adherence and have shown that the disruption of C. albicans IFF4 gene encoding a GPI-anchor protein, led to a decrease of adherence of the germ tubes to plastic. Here, we demonstrated the role of the IFF4 gene in adherence to silicone catheter, as well as in virulence using a murine model of disseminated candidiasis. The iff4 Delta null mutant showed both a decrease of adherence to silicone catheter and a reduction of virulence. This work presents evidence for the importance of the IFF4 gene in host-fungal interaction.
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Among the regulated cell wall genes were homologues of C. albicans glucanase genes (XOG1 and ENG1) as well as genes encoding putative adhesins (ALS4, IFF4, and PGA6).
“Among the regulated cell wall genes were homologues of C. albicans glucanase genes (XOG1 and ENG1) (46, 47) as well as genes encoding putative adhesins (ALS4, IFF4, and PGA6) (48–50).”
From Transcriptomics and Phenotyping Define Genetic Signatures Associated with Echinocandin Resistance in Candida auris (2022) · cites this paper as [48]
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Adhesins in human fungal pathogens: glue with plenty of stick.
de Groot, P. W. J., Bader, O., de Boer, A. D., Weig, M., & Chauhan, N. (2013) · Eukaryotic Cell · doi:10.1128/ec.00364-12 · Cited by 319 · see citing works
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- Authors
- Kempf, M., Cottin, J., Licznar, P., Lefrançois, C., Robert, R., & Apaire-Marchais, V.
- Affiliations
- Université d'Angers (FR)
- Centre Hospitalier Universitaire d'Angers (FR)
- Corresponding author
- Kempf, Marie
- ORCID
- Kempf, Marie
- Venue
- Mycopathologia
- Published
- 2009-04-05
- Volume
- 168
- Issue
- 2
- Pages
- 73-77
- ISSN
- 0301-486X (ISSN-L)
- 1573-0832
- OpenAlex
- W1989079653
- Cited by
- 33
- References
- 4
- Citations by year
- 2026: 2 · 2025: 1 · 2024: 2 · 2023: 2 · 2022: 1 · 2021: 4 · 2019: 5 · 2018: 2 · 2016: 2 · 2014: 4 · 2013: 1 · 2012: 3
- FWCI
- 12.83
- Type
- book chapter
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Mycopathologia (publishedVersion)
- PubMed (publishedVersion)
- HAL (submittedVersion)
- Topics
- Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
- Health Sciences › Medicine › Epidemiology › Fungal Infections and Studies
- Keywords
- Candida albicans, silicone catheters, systemic candidiasis, virulence, fungal-host interactions, gene disruption mutants
- MeSH terms
- Animals, Candida albicans, Candidiasis, Catheterization, Cell Adhesion, Equipment and Supplies, Female, Fungal Proteins, Genes, Fungal, Virulence, Colony Count, Microbial, Survival Analysis, Virulence Factors, Mice, Gene Knockout Techniques
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