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Cited by 22Antifungal resistance and susceptibilityYeasts and Rust Fungi StudiesFungal Infections and Studies

Occurrence and Characterization of Candida nivariensis from a Culture Collection of Candida glabrata Clinical Isolates in Malaysia

Tay, Sun Tee, Lotfalikhani, Azadeh, Sabet, Negar Shafiei, Ponnampalavanar, Sasheela, Sulaiman, Sofiah, Na, Shiang Ling, Ng, Kee Peng·2014·Mycopathologia
DOI 10.1007/s11046-014-9778-9
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Abstract

Background Candida nivariensis and C. bracarensis have been recently identified as emerging yeast pathogens which are phenotypically indistinguishable from C. glabrata. However, there is little data on the prevalence and antifungal susceptibilities of these species.Objective This study investigated the occurrence of C. nivariensis and C. bracarensis in a culture collection of 185 C. glabrata isolates at a Malaysian teaching hospital.Methods C. nivariensis was discriminated from C. glabrata using a PCR assay as described by Enache-Angoulvant et al. (J Clin Microbiol 49:3375-9, 2011). The identity of the isolates was confirmed by sequence analysis of the D1D2 domain and internal transcribed spacer region of the yeasts. The isolates were cultured on Chromogenic CHROMagar Candida (®) agar (Difco, USA), and their biochemical and enzymic profiles were determined. Antifungal susceptibilities of the isolates against amphotericin B, fluconazole, voriconazole and caspofungin were determined using E tests. Clotrimazole MICs were determined using a microbroth dilution method.Results There was a low prevalence (1.1 %) of C. nivariensis in our culture collection of C. glabrata. C. nivariensis was isolated from a blood culture and vaginal swab of two patients. C. nivariensis grew as white colonies on Chromogenic agar and demonstrated few positive reactions using biochemical tests. Enzymatic profiles of the C. nivariensis isolates were similar to that of C. glabrata. The isolates were susceptible to amphotericin B, fluconazole, voriconazole and caspofungin. Clotrimazole resistance is suspected in one isolate.Conclusion This study reports for the first time the emergence of C. nivariensis in our clinical setting.

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  • Tay et al. reported that C. glabrata isolates demonstrated similar MIC50 (0.25 μg/mL) against amphotericin B for biofilm and planktonic cells.

    “Tay et al. [110] reported that C. glabrata isolates demonstrated similar MIC50 (0.25 μg/mL) against AmB for biofilm and planktonic cells.”

    From Candida glabrata: pathogenicity and resistance mechanisms for adaptation and survival (2021) · cites this paper as [110]

  • The findings attributed lower resistance of C. glabrata with biofilms against amphotericin and not about the low biofilm content of the isolates tested.

    “The findings attributed lower resistance of C. glabrata with biofilms against amphotericin and not about the low biofilm content of the isolates tested.”

    From Candida glabrata: pathogenicity and resistance mechanisms for adaptation and survival (2021) · cites this paper as [110]

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Authors
Tay, S. T., Lotfalikhani, A., Sabet, N. S., Ponnampalavanar, S., Sulaiman, S., Na, S. L., & Ng, K. P.
Affiliations
University of Malaya (MY)
Corresponding author
Tay, Sun Tee
ORCID
Venue
Mycopathologia
Publisher
Springer Science and Business Media LLC
Published
2014-07-15
Volume
178
Issue
3-4
Pages
307-314
ISSN
  • 0301-486X (ISSN-L)
  • 1573-0832
DOI
10.1007/s11046-014-9778-9
OpenAlex
W1987360980
Cited by
22
References
31
Citations by year
2024: 2 · 2022: 2 · 2021: 2 · 2020: 4 · 2019: 4 · 2018: 3 · 2016: 2 · 2015: 3
FWCI
1.17
Type
journal article
Language
en
Open access
closed
Licence
www.springer.com/tdm (tdm)
Locations
  • Mycopathologia (publishedVersion)
  • PubMed (publishedVersion)
Funders
Universiti Malaya
Topics
  • Health Sciences › Medicine › Infectious Diseases › Antifungal resistance and susceptibility
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Yeasts and Rust Fungi Studies
  • Health Sciences › Medicine › Epidemiology › Fungal Infections and Studies
Keywords
Candida nivariensis, Candida bracarensis, Candida glabrata, antifungal susceptibility, clotrimazole resistance, ITS region, clinical isolates, Malaysia
MeSH terms
Adult, Aged, Antifungal Agents, Candida, Candidiasis, Culture Media, DNA, Fungal, Hospitals, Teaching, Humans, Malaysia, Microbial Sensitivity Tests, Microbiological Techniques, Molecular Sequence Data, Phylogeny, Cluster Analysis, Polymerase Chain Reaction, Mycological Typing Techniques, Sequence Analysis, DNA, DNA, Ribosomal Spacer

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